High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity

Recent advances in high throughput sequencing (HTS) of T cell receptors (TCRs) and in transcriptomic analysis, particularly at the single cell level, have opened the door to a new level of understanding of human immunology and immune-related diseases. In this article, we discuss the use of HTS of TC...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jianing Fu, Mohsen Khosravi-Maharlooei, Megan Sykes
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	human alloresponse organ transplantation high throughput TCR sequencing human immune system mouse models thymic selection immune tolerance Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/e4c7a75d1eba4470b5d1bbf494a599a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e4c7a75d1eba4470b5d1bbf494a599a7
record_format	dspace
spelling	oai:doaj.org-article:e4c7a75d1eba4470b5d1bbf494a599a72021-11-05T12:01:51ZHigh Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity1664-322410.3389/fimmu.2021.777756https://doaj.org/article/e4c7a75d1eba4470b5d1bbf494a599a72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.777756/fullhttps://doaj.org/toc/1664-3224Recent advances in high throughput sequencing (HTS) of T cell receptors (TCRs) and in transcriptomic analysis, particularly at the single cell level, have opened the door to a new level of understanding of human immunology and immune-related diseases. In this article, we discuss the use of HTS of TCRs to discern the factors controlling human T cell repertoire development and how this approach can be used in combination with human immune system (HIS) mouse models to understand human repertoire selection in an unprecedented manner. An exceptionally high proportion of human T cells has alloreactive potential, which can best be understood as a consequence of the processes governing thymic selection. High throughput TCR sequencing has allowed assessment of the development, magnitude and nature of the human alloresponse at a new level and has provided a tool for tracking the fate of pre-transplant-defined donor- and host-reactive TCRs following transplantation. New insights into human allograft rejection and tolerance obtained with this method in combination with single cell transcriptional analyses are reviewed here.Jianing FuMohsen Khosravi-MaharlooeiMegan SykesMegan SykesMegan SykesFrontiers Media S.A.articlehuman alloresponseorgan transplantationhigh throughput TCR sequencinghuman immune system mouse modelsthymic selectionimmune toleranceImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human alloresponse organ transplantation high throughput TCR sequencing human immune system mouse models thymic selection immune tolerance Immunologic diseases. Allergy RC581-607
spellingShingle	human alloresponse organ transplantation high throughput TCR sequencing human immune system mouse models thymic selection immune tolerance Immunologic diseases. Allergy RC581-607 Jianing Fu Mohsen Khosravi-Maharlooei Megan Sykes Megan Sykes Megan Sykes High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
description	Recent advances in high throughput sequencing (HTS) of T cell receptors (TCRs) and in transcriptomic analysis, particularly at the single cell level, have opened the door to a new level of understanding of human immunology and immune-related diseases. In this article, we discuss the use of HTS of TCRs to discern the factors controlling human T cell repertoire development and how this approach can be used in combination with human immune system (HIS) mouse models to understand human repertoire selection in an unprecedented manner. An exceptionally high proportion of human T cells has alloreactive potential, which can best be understood as a consequence of the processes governing thymic selection. High throughput TCR sequencing has allowed assessment of the development, magnitude and nature of the human alloresponse at a new level and has provided a tool for tracking the fate of pre-transplant-defined donor- and host-reactive TCRs following transplantation. New insights into human allograft rejection and tolerance obtained with this method in combination with single cell transcriptional analyses are reviewed here.
format	article
author	Jianing Fu Mohsen Khosravi-Maharlooei Megan Sykes Megan Sykes Megan Sykes
author_facet	Jianing Fu Mohsen Khosravi-Maharlooei Megan Sykes Megan Sykes Megan Sykes
author_sort	Jianing Fu
title	High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
title_short	High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
title_full	High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
title_fullStr	High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
title_full_unstemmed	High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity
title_sort	high throughput human t cell receptor sequencing: a new window into repertoire establishment and alloreactivity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e4c7a75d1eba4470b5d1bbf494a599a7
work_keys_str_mv	AT jianingfu highthroughputhumantcellreceptorsequencinganewwindowintorepertoireestablishmentandalloreactivity AT mohsenkhosravimaharlooei highthroughputhumantcellreceptorsequencinganewwindowintorepertoireestablishmentandalloreactivity AT megansykes highthroughputhumantcellreceptorsequencinganewwindowintorepertoireestablishmentandalloreactivity AT megansykes highthroughputhumantcellreceptorsequencinganewwindowintorepertoireestablishmentandalloreactivity AT megansykes highthroughputhumantcellreceptorsequencinganewwindowintorepertoireestablishmentandalloreactivity
_version_	1718444321451016192

High Throughput Human T Cell Receptor Sequencing: A New Window Into Repertoire Establishment and Alloreactivity

Ejemplares similares