Active-site loop variations adjust activity and selectivity of the cumene dioxygenase

Active-site loops are important for catalytic properties of enzymes, but challenging to engineer due to their high flexibility and diversity. Here, the authors identify and engineer hot-spots in the loops of cumene dioxygenase, obtain variants with changed activity, regio- and enantioselectivity, an...

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Autores principales:	Peter M. Heinemann, Daniel Armbruster, Bernhard Hauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e4cdf7460e154bfdbc8afe31e3043963
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spelling	oai:doaj.org-article:e4cdf7460e154bfdbc8afe31e30439632021-12-02T14:21:30ZActive-site loop variations adjust activity and selectivity of the cumene dioxygenase10.1038/s41467-021-21328-82041-1723https://doaj.org/article/e4cdf7460e154bfdbc8afe31e30439632021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21328-8https://doaj.org/toc/2041-1723Active-site loops are important for catalytic properties of enzymes, but challenging to engineer due to their high flexibility and diversity. Here, the authors identify and engineer hot-spots in the loops of cumene dioxygenase, obtain variants with changed activity, regio- and enantioselectivity, and present a Linker In Loop Insertion approach for loop modification.Peter M. HeinemannDaniel ArmbrusterBernhard HauerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Peter M. Heinemann Daniel Armbruster Bernhard Hauer Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
description	Active-site loops are important for catalytic properties of enzymes, but challenging to engineer due to their high flexibility and diversity. Here, the authors identify and engineer hot-spots in the loops of cumene dioxygenase, obtain variants with changed activity, regio- and enantioselectivity, and present a Linker In Loop Insertion approach for loop modification.
format	article
author	Peter M. Heinemann Daniel Armbruster Bernhard Hauer
author_facet	Peter M. Heinemann Daniel Armbruster Bernhard Hauer
author_sort	Peter M. Heinemann
title	Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
title_short	Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
title_full	Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
title_fullStr	Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
title_full_unstemmed	Active-site loop variations adjust activity and selectivity of the cumene dioxygenase
title_sort	active-site loop variations adjust activity and selectivity of the cumene dioxygenase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4cdf7460e154bfdbc8afe31e3043963
work_keys_str_mv	AT petermheinemann activesiteloopvariationsadjustactivityandselectivityofthecumenedioxygenase AT danielarmbruster activesiteloopvariationsadjustactivityandselectivityofthecumenedioxygenase AT bernhardhauer activesiteloopvariationsadjustactivityandselectivityofthecumenedioxygenase
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Active-site loop variations adjust activity and selectivity of the cumene dioxygenase

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