Differential micro RNA expression in PBMC from multiple sclerosis patients.

Differences in gene expression patterns have been documented not only in Multiple Sclerosis patients versus healthy controls but also in the relapse of the disease. Recently a new gene expression modulator has been identified: the microRNA or miRNA. The aim of this work is to analyze the possible ro...

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Autores principales:	David Otaegui, Sergio E Baranzini, Ruben Armañanzas, Borja Calvo, Maider Muñoz-Culla, Puya Khankhanian, Iñaki Inza, Jose A Lozano, Tamara Castillo-Triviño, Ana Asensio, Javier Olaskoaga, Adolfo López de Munain
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4cf60b20795407283e940b245025d94
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spelling	oai:doaj.org-article:e4cf60b20795407283e940b245025d942021-11-25T06:21:31ZDifferential micro RNA expression in PBMC from multiple sclerosis patients.1932-620310.1371/journal.pone.0006309https://doaj.org/article/e4cf60b20795407283e940b245025d942009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19617918/?tool=EBIhttps://doaj.org/toc/1932-6203Differences in gene expression patterns have been documented not only in Multiple Sclerosis patients versus healthy controls but also in the relapse of the disease. Recently a new gene expression modulator has been identified: the microRNA or miRNA. The aim of this work is to analyze the possible role of miRNAs in multiple sclerosis, focusing on the relapse stage. We have analyzed the expression patterns of 364 miRNAs in PBMC obtained from multiple sclerosis patients in relapse status, in remission status and healthy controls. The expression patterns of the miRNAs with significantly different expression were validated in an independent set of samples. In order to determine the effect of the miRNAs, the expression of some predicted target genes of these were studied by qPCR. Gene interaction networks were constructed in order to obtain a co-expression and multivariate view of the experimental data. The data analysis and later validation reveal that two miRNAs (hsa-miR-18b and hsa-miR-599) may be relevant at the time of relapse and that another miRNA (hsa-miR-96) may be involved in remission. The genes targeted by hsa-miR-96 are involved in immunological pathways as Interleukin signaling and in other pathways as wnt signaling. This work highlights the importance of miRNA expression in the molecular mechanisms implicated in the disease. Moreover, the proposed involvement of these small molecules in multiple sclerosis opens up a new therapeutic approach to explore and highlight some candidate biomarker targets in MS.David OtaeguiSergio E BaranziniRuben ArmañanzasBorja CalvoMaider Muñoz-CullaPuya KhankhanianIñaki InzaJose A LozanoTamara Castillo-TriviñoAna AsensioJavier OlaskoagaAdolfo López de MunainPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6309 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Otaegui Sergio E Baranzini Ruben Armañanzas Borja Calvo Maider Muñoz-Culla Puya Khankhanian Iñaki Inza Jose A Lozano Tamara Castillo-Triviño Ana Asensio Javier Olaskoaga Adolfo López de Munain Differential micro RNA expression in PBMC from multiple sclerosis patients.
description	Differences in gene expression patterns have been documented not only in Multiple Sclerosis patients versus healthy controls but also in the relapse of the disease. Recently a new gene expression modulator has been identified: the microRNA or miRNA. The aim of this work is to analyze the possible role of miRNAs in multiple sclerosis, focusing on the relapse stage. We have analyzed the expression patterns of 364 miRNAs in PBMC obtained from multiple sclerosis patients in relapse status, in remission status and healthy controls. The expression patterns of the miRNAs with significantly different expression were validated in an independent set of samples. In order to determine the effect of the miRNAs, the expression of some predicted target genes of these were studied by qPCR. Gene interaction networks were constructed in order to obtain a co-expression and multivariate view of the experimental data. The data analysis and later validation reveal that two miRNAs (hsa-miR-18b and hsa-miR-599) may be relevant at the time of relapse and that another miRNA (hsa-miR-96) may be involved in remission. The genes targeted by hsa-miR-96 are involved in immunological pathways as Interleukin signaling and in other pathways as wnt signaling. This work highlights the importance of miRNA expression in the molecular mechanisms implicated in the disease. Moreover, the proposed involvement of these small molecules in multiple sclerosis opens up a new therapeutic approach to explore and highlight some candidate biomarker targets in MS.
format	article
author	David Otaegui Sergio E Baranzini Ruben Armañanzas Borja Calvo Maider Muñoz-Culla Puya Khankhanian Iñaki Inza Jose A Lozano Tamara Castillo-Triviño Ana Asensio Javier Olaskoaga Adolfo López de Munain
author_facet	David Otaegui Sergio E Baranzini Ruben Armañanzas Borja Calvo Maider Muñoz-Culla Puya Khankhanian Iñaki Inza Jose A Lozano Tamara Castillo-Triviño Ana Asensio Javier Olaskoaga Adolfo López de Munain
author_sort	David Otaegui
title	Differential micro RNA expression in PBMC from multiple sclerosis patients.
title_short	Differential micro RNA expression in PBMC from multiple sclerosis patients.
title_full	Differential micro RNA expression in PBMC from multiple sclerosis patients.
title_fullStr	Differential micro RNA expression in PBMC from multiple sclerosis patients.
title_full_unstemmed	Differential micro RNA expression in PBMC from multiple sclerosis patients.
title_sort	differential micro rna expression in pbmc from multiple sclerosis patients.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/e4cf60b20795407283e940b245025d94
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_version_	1718413833534439424

Differential micro RNA expression in PBMC from multiple sclerosis patients.

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