Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression
Abstract Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims to take advantage of plant functional plasticity towards stimulation of plant agricultural performance. In this study, the effects of pre-sowing seed treatment using 200 Pa vacuum (7 min), 5.28 MHz radio-freque...
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Nature Portfolio
2019
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oai:doaj.org-article:e4d2dc59dc2b469c96f2fa55efe4e0a42021-12-02T15:07:53ZTreatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression10.1038/s41598-019-42893-52045-2322https://doaj.org/article/e4d2dc59dc2b469c96f2fa55efe4e0a42019-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-42893-5https://doaj.org/toc/2045-2322Abstract Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims to take advantage of plant functional plasticity towards stimulation of plant agricultural performance. In this study, the effects of pre-sowing seed treatment using 200 Pa vacuum (7 min), 5.28 MHz radio-frequency cold plasma (CP −2, 5, and 7 min) and electromagnetic field (EMF −5, 10, 15 min) on seed germination kinetics, content of phytohormones, morphometric parameters of seedlings and leaf proteome were assessed. CP 7 min and EMF 15 min treatments caused 19–24% faster germination in vitro; germination in the substrate was accelerated by vacuum (9%) and EMF 15 min (17%). The stressors did not change the seed germination percentage, with exception of EMF 5 min treatment that caused a decrease by 7.5%. Meanwhile both CP 7 min and EMF 15 min treatments stimulated germination, but the EMF treatment resulted in higher weight of leaves. Stressor-specific changes in phytohormone balance were detected in seeds: vacuum treatment decreased zeatin amount by 39%; CP treatments substantially increased gibberellin content, but other effects strongly varied with the treatment duration; the abscisic acid content was reduced by 55–60% after the EMF treatment. Analysis of the proteome showed that short exposure of seeds to the EMF or CP induced a similar long-term effect on gene expression in leaves, mostly stimulating expression of proteins involved in photosynthetic processes and their regulation.Vida MildažienėVesta AleknavičiūtėRasa ŽūkienėGiedrė PaužaitėZita NaučienėIrina FilatovaVeronika LyushkevichPerttu HaimiInga TamošiūnėDanas BaniulisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
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Medicine R Science Q Vida Mildažienė Vesta Aleknavičiūtė Rasa Žūkienė Giedrė Paužaitė Zita Naučienė Irina Filatova Veronika Lyushkevich Perttu Haimi Inga Tamošiūnė Danas Baniulis Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| description |
Abstract Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims to take advantage of plant functional plasticity towards stimulation of plant agricultural performance. In this study, the effects of pre-sowing seed treatment using 200 Pa vacuum (7 min), 5.28 MHz radio-frequency cold plasma (CP −2, 5, and 7 min) and electromagnetic field (EMF −5, 10, 15 min) on seed germination kinetics, content of phytohormones, morphometric parameters of seedlings and leaf proteome were assessed. CP 7 min and EMF 15 min treatments caused 19–24% faster germination in vitro; germination in the substrate was accelerated by vacuum (9%) and EMF 15 min (17%). The stressors did not change the seed germination percentage, with exception of EMF 5 min treatment that caused a decrease by 7.5%. Meanwhile both CP 7 min and EMF 15 min treatments stimulated germination, but the EMF treatment resulted in higher weight of leaves. Stressor-specific changes in phytohormone balance were detected in seeds: vacuum treatment decreased zeatin amount by 39%; CP treatments substantially increased gibberellin content, but other effects strongly varied with the treatment duration; the abscisic acid content was reduced by 55–60% after the EMF treatment. Analysis of the proteome showed that short exposure of seeds to the EMF or CP induced a similar long-term effect on gene expression in leaves, mostly stimulating expression of proteins involved in photosynthetic processes and their regulation. |
| format |
article |
| author |
Vida Mildažienė Vesta Aleknavičiūtė Rasa Žūkienė Giedrė Paužaitė Zita Naučienė Irina Filatova Veronika Lyushkevich Perttu Haimi Inga Tamošiūnė Danas Baniulis |
| author_facet |
Vida Mildažienė Vesta Aleknavičiūtė Rasa Žūkienė Giedrė Paužaitė Zita Naučienė Irina Filatova Veronika Lyushkevich Perttu Haimi Inga Tamošiūnė Danas Baniulis |
| author_sort |
Vida Mildažienė |
| title |
Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| title_short |
Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| title_full |
Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| title_fullStr |
Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| title_full_unstemmed |
Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression |
| title_sort |
treatment of common sunflower (helianthus annus l.) seeds with radio-frequency electromagnetic field and cold plasma induces changes in seed phytohormone balance, seedling development and leaf protein expression |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/e4d2dc59dc2b469c96f2fa55efe4e0a4 |
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