Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study

Abstract Peripheral blood mononuclear cells (PBMC) are widely used as a biomarker source in nutrition/obesity studies because they reflect gene expression profiles of internal tissues. In this pilot proof-of-concept study we analysed in humans if, as we previously suggested in rodents, PBMC could be...

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Autores principales:	Andrea Costa, Bàrbara Reynés, Jadwiga Konieczna, Marian Martín, Miquel Fiol, Andreu Palou, Dora Romaguera, Paula Oliver
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4d4d4bd678e4b1484866e2ab7284619
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spelling	oai:doaj.org-article:e4d4d4bd678e4b1484866e2ab72846192021-12-02T18:50:52ZUse of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study10.1038/s41598-021-96981-62045-2322https://doaj.org/article/e4d4d4bd678e4b1484866e2ab72846192021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96981-6https://doaj.org/toc/2045-2322Abstract Peripheral blood mononuclear cells (PBMC) are widely used as a biomarker source in nutrition/obesity studies because they reflect gene expression profiles of internal tissues. In this pilot proof-of-concept study we analysed in humans if, as we previously suggested in rodents, PBMC could be a surrogate tissue to study overweight/obesity impact on lipid metabolism. Pre-selected key lipid metabolism genes based in our previous preclinical studies were analysed in PBMC of normoglycemic normal-weight (NW), and overweight-obese (OW-OB) subjects before and after a 6-month weight-loss plan. PBMC mRNA levels of CPT1A, FASN and SREBP-1c increased in the OW-OB group, according with what described in liver and adipose tissue of humans with obesity. This altered expression pattern was related to increased adiposity and early signs of metabolic impairment. Greater weight loss and/or metabolic improvement as result of the intervention was related to lower CPT1A, FASN and SREBP-1c gene expression in an adjusted linear mixed-effects regression analysis, although no gene expression recovery was observed when considering mean comparisons. Thus, human PBMC reflect lipid metabolism expression profile of energy homeostatic tissues, and early obesity-related alterations in metabolic at-risk subjects. Further studies are needed to understand PBMC usefulness for analysis of metabolic recovery in weigh management programs.Andrea CostaBàrbara ReynésJadwiga KoniecznaMarian MartínMiquel FiolAndreu PalouDora RomagueraPaula OliverNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Costa Bàrbara Reynés Jadwiga Konieczna Marian Martín Miquel Fiol Andreu Palou Dora Romaguera Paula Oliver Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
description	Abstract Peripheral blood mononuclear cells (PBMC) are widely used as a biomarker source in nutrition/obesity studies because they reflect gene expression profiles of internal tissues. In this pilot proof-of-concept study we analysed in humans if, as we previously suggested in rodents, PBMC could be a surrogate tissue to study overweight/obesity impact on lipid metabolism. Pre-selected key lipid metabolism genes based in our previous preclinical studies were analysed in PBMC of normoglycemic normal-weight (NW), and overweight-obese (OW-OB) subjects before and after a 6-month weight-loss plan. PBMC mRNA levels of CPT1A, FASN and SREBP-1c increased in the OW-OB group, according with what described in liver and adipose tissue of humans with obesity. This altered expression pattern was related to increased adiposity and early signs of metabolic impairment. Greater weight loss and/or metabolic improvement as result of the intervention was related to lower CPT1A, FASN and SREBP-1c gene expression in an adjusted linear mixed-effects regression analysis, although no gene expression recovery was observed when considering mean comparisons. Thus, human PBMC reflect lipid metabolism expression profile of energy homeostatic tissues, and early obesity-related alterations in metabolic at-risk subjects. Further studies are needed to understand PBMC usefulness for analysis of metabolic recovery in weigh management programs.
format	article
author	Andrea Costa Bàrbara Reynés Jadwiga Konieczna Marian Martín Miquel Fiol Andreu Palou Dora Romaguera Paula Oliver
author_facet	Andrea Costa Bàrbara Reynés Jadwiga Konieczna Marian Martín Miquel Fiol Andreu Palou Dora Romaguera Paula Oliver
author_sort	Andrea Costa
title	Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
title_short	Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
title_full	Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
title_fullStr	Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
title_full_unstemmed	Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
title_sort	use of human pbmc to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4d4d4bd678e4b1484866e2ab7284619
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Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study

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