Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract

Mohamad S AlSalhi,1,2 Sandhanasamy Devanesan,1,2 Muhammad Atif,1,2 Wedad S AlQahtani,3 Marcello Nicoletti,4 Paola Del Serrone5 1Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia; 2Physics...

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Autores principales:	AlSalhi MS, Devanesan S, Atif M, AlQahtani WS, Nicoletti M, Serrone PD
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	fennel seed extract derived zinc oxide nanoparticles green nanotechnology anti-tumor activity antimicrobial activity and therapeutic agents. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e4db6a8890eb488faf50280925e6db3d
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spelling	oai:doaj.org-article:e4db6a8890eb488faf50280925e6db3d2021-12-02T15:28:27ZTherapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract1178-2013https://doaj.org/article/e4db6a8890eb488faf50280925e6db3d2020-10-01T00:00:00Zhttps://www.dovepress.com/therapeutic-potential-assessment-of-green-synthesized-zinc-oxide-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mohamad S AlSalhi,1,2 Sandhanasamy Devanesan,1,2 Muhammad Atif,1,2 Wedad S AlQahtani,3 Marcello Nicoletti,4 Paola Del Serrone5 1Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia; 2Physics and Astronomy Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 3Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, 11451, Saudi Arabia; 4Department of Environmental Biology, Sapienza University of Rome, Rome 00185, Italy; 5Council for Agricultural Research and Economics, Research Center for Zootechnic and Aquaculture, Monterotondo, Rome, 00015, ItalyCorrespondence: Sandhanasamy Devanesan; Muhammad AtifResearch Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi ArabiaEmail sdnesan1981@gmail.comPurpose: To study the cytotoxic evaluation, antimicrobial and confocal analysis of zinc oxide nanoparticles (ZnO NPs) obtained from a novel plant product fennel (Foeniculum vulgare Mill.) seed extract (FSE).Methods: ZnO NPs were analyzed using UV–Vis spectroscopy, XRD, FTIR, TEM and EDX techniques. The MTT cell cytotoxicity assay measured the proliferation and survival of MCF-7 cells treated at different concentrations of FSE-derived ZnO NPs. The antimicrobial activity towards pathogenic bacteria and yeast strains was investigated.Results: The UV–Vis spectra showed two peaks at 438 nm and 446 nm, confirming nanoparticle formation. The SEM morphology results showed porous ranging from 23– 51 nm. The antitumor activity value (IC50) was at 50 μg/mL and 100 μg/mL. Besides, morphological changes of MCF-7, cells treated at different concentrations of FSE of ZnO NPs were observed in cell cultures transfected with a transient pCMV6-XL4-GFP-expressing vector containing C-terminal domain GFP-tagged proteins, which resulted in an apoptotic effect. Antimicrobial IZ ranged up No Inhibition to 18.00 ± 0.4. The IZ revealed at the highest concentration was E. faecium VRE and yeast Cryptococcus sp. (18.00 ± 0.4. mm), followed by S. aureus (17.00 ± 0.2 mm) and P. aeruginosa and the yeast C. parapsilosis (16 ± 0.4 mm). The IZ was equal to that caused by the nystatin to Cryptococcus sp., which was significantly highest than ampicillin treatments of S. aureus, P. aeruginosa, C. albicans, and C. parapsilosis. The MIC value of the FSE-derived ZnO NPs tested against E.faecium and C.albicans was 6.00 μg/mL (E. faecium and C. albicans). It was 32.00 μg/mL (S. aureus, S. typhimurium and Cryptococcus sp.), 64.00 μg/mL (P. aeruginosa), and 128 μg/mL (C. parapsilosis).Conclusion: As far as it is to our knowledge, this study established, for the first time, the biological activities of biosynthesized ZnO NPs from FSE and their synergistic therapeutic potential.Keywords: fennel seed extract-derived zinc oxide nanoparticles, green nanotechnology, anti-tumor activity, antimicrobial activity and therapeutic agentsAlSalhi MSDevanesan SAtif MAlQahtani WSNicoletti MSerrone PDDove Medical Pressarticlefennel seed extract derived zinc oxide nanoparticlesgreen nanotechnologyanti-tumor activityantimicrobial activity and therapeutic agents.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8045-8057 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fennel seed extract derived zinc oxide nanoparticles green nanotechnology anti-tumor activity antimicrobial activity and therapeutic agents. Medicine (General) R5-920
spellingShingle	fennel seed extract derived zinc oxide nanoparticles green nanotechnology anti-tumor activity antimicrobial activity and therapeutic agents. Medicine (General) R5-920 AlSalhi MS Devanesan S Atif M AlQahtani WS Nicoletti M Serrone PD Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
description	Mohamad S AlSalhi,1,2 Sandhanasamy Devanesan,1,2 Muhammad Atif,1,2 Wedad S AlQahtani,3 Marcello Nicoletti,4 Paola Del Serrone5 1Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia; 2Physics and Astronomy Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 3Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, 11451, Saudi Arabia; 4Department of Environmental Biology, Sapienza University of Rome, Rome 00185, Italy; 5Council for Agricultural Research and Economics, Research Center for Zootechnic and Aquaculture, Monterotondo, Rome, 00015, ItalyCorrespondence: Sandhanasamy Devanesan; Muhammad AtifResearch Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi ArabiaEmail sdnesan1981@gmail.comPurpose: To study the cytotoxic evaluation, antimicrobial and confocal analysis of zinc oxide nanoparticles (ZnO NPs) obtained from a novel plant product fennel (Foeniculum vulgare Mill.) seed extract (FSE).Methods: ZnO NPs were analyzed using UV–Vis spectroscopy, XRD, FTIR, TEM and EDX techniques. The MTT cell cytotoxicity assay measured the proliferation and survival of MCF-7 cells treated at different concentrations of FSE-derived ZnO NPs. The antimicrobial activity towards pathogenic bacteria and yeast strains was investigated.Results: The UV–Vis spectra showed two peaks at 438 nm and 446 nm, confirming nanoparticle formation. The SEM morphology results showed porous ranging from 23– 51 nm. The antitumor activity value (IC50) was at 50 μg/mL and 100 μg/mL. Besides, morphological changes of MCF-7, cells treated at different concentrations of FSE of ZnO NPs were observed in cell cultures transfected with a transient pCMV6-XL4-GFP-expressing vector containing C-terminal domain GFP-tagged proteins, which resulted in an apoptotic effect. Antimicrobial IZ ranged up No Inhibition to 18.00 ± 0.4. The IZ revealed at the highest concentration was E. faecium VRE and yeast Cryptococcus sp. (18.00 ± 0.4. mm), followed by S. aureus (17.00 ± 0.2 mm) and P. aeruginosa and the yeast C. parapsilosis (16 ± 0.4 mm). The IZ was equal to that caused by the nystatin to Cryptococcus sp., which was significantly highest than ampicillin treatments of S. aureus, P. aeruginosa, C. albicans, and C. parapsilosis. The MIC value of the FSE-derived ZnO NPs tested against E.faecium and C.albicans was 6.00 μg/mL (E. faecium and C. albicans). It was 32.00 μg/mL (S. aureus, S. typhimurium and Cryptococcus sp.), 64.00 μg/mL (P. aeruginosa), and 128 μg/mL (C. parapsilosis).Conclusion: As far as it is to our knowledge, this study established, for the first time, the biological activities of biosynthesized ZnO NPs from FSE and their synergistic therapeutic potential.Keywords: fennel seed extract-derived zinc oxide nanoparticles, green nanotechnology, anti-tumor activity, antimicrobial activity and therapeutic agents
format	article
author	AlSalhi MS Devanesan S Atif M AlQahtani WS Nicoletti M Serrone PD
author_facet	AlSalhi MS Devanesan S Atif M AlQahtani WS Nicoletti M Serrone PD
author_sort	AlSalhi MS
title	Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
title_short	Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
title_full	Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
title_fullStr	Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
title_full_unstemmed	Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract
title_sort	therapeutic potential assessment of green synthesized zinc oxide nanoparticles derived from fennel seeds extract
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/e4db6a8890eb488faf50280925e6db3d
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Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract

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