Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells

We previously reported an anti-fibrogenic effects of fucoxanthin in hepatic stellate cells (HSCs). However, it is unknown whether the primary metabolites of fucoxanthin, such as fucoxanthinol (FCN) and amarouciaxanthin A (ACXA), exert similar functions in fibrogenesis pathways. In this study, we inv...

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Autores principales:	Yang Li, Mi-Bo Kim, Young-Ki Park, Ji-Young Lee
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Fucoxanthin Fucoxanthinol Amarouciaxanthin A Anti-fibrogenic Antioxidant Hepatic stellate cell Agriculture (General) S1-972 Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/e4deeafcca9745c5aad33f2fb3765696
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spelling	oai:doaj.org-article:e4deeafcca9745c5aad33f2fb37656962021-12-02T05:03:42ZFucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells2666-154310.1016/j.jafr.2021.100245https://doaj.org/article/e4deeafcca9745c5aad33f2fb37656962021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666154321001472https://doaj.org/toc/2666-1543We previously reported an anti-fibrogenic effects of fucoxanthin in hepatic stellate cells (HSCs). However, it is unknown whether the primary metabolites of fucoxanthin, such as fucoxanthinol (FCN) and amarouciaxanthin A (ACXA), exert similar functions in fibrogenesis pathways. In this study, we investigated the role of FCN and ACXA in the regulation of transforming growth factor β1 (TGFβ1)-induced pro-fibrogenic gene expression and oxidative stress in LX-2 cells, a human HSC cell line. TGFβ1 increased mRNA expression of pro-fibrogenic genes, such as α smooth muscle actin, procollagen type I α1 (COL1A1), COL3A1, and COL6A1, which FCN and ACXA significantly suppressed. Also, an increase in the protein level of COL1A1 by TGFβ1 was markedly decreased by ACXA. Furthermore, FCN and ACXA significantly inhibited TGFβ1-induced reactive oxygen species (ROS) accumulation with concomitantly decreasing antioxidant gene expression. Mechanistically, FCN and ACXA markedly increased TGFβ1-induced nuclear translocation of nuclear E2-related factor 2 (NRF2); however, they significantly attenuated TGFβ1-induced NFE2L2 and its target gene heme oxygenase-1 expression. The results suggest that FCN and ACXA exert anti-fibrogenic and antioxidant properties by inhibiting TGFβ1-induced pro-fibrogenic gene expression and ROS accumulation in LX-2 cells.Yang LiMi-Bo KimYoung-Ki ParkJi-Young LeeElsevierarticleFucoxanthinFucoxanthinolAmarouciaxanthin AAnti-fibrogenicAntioxidantHepatic stellate cellAgriculture (General)S1-972Nutrition. Foods and food supplyTX341-641ENJournal of Agriculture and Food Research, Vol 6, Iss , Pp 100245- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fucoxanthin Fucoxanthinol Amarouciaxanthin A Anti-fibrogenic Antioxidant Hepatic stellate cell Agriculture (General) S1-972 Nutrition. Foods and food supply TX341-641
spellingShingle	Fucoxanthin Fucoxanthinol Amarouciaxanthin A Anti-fibrogenic Antioxidant Hepatic stellate cell Agriculture (General) S1-972 Nutrition. Foods and food supply TX341-641 Yang Li Mi-Bo Kim Young-Ki Park Ji-Young Lee Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
description	We previously reported an anti-fibrogenic effects of fucoxanthin in hepatic stellate cells (HSCs). However, it is unknown whether the primary metabolites of fucoxanthin, such as fucoxanthinol (FCN) and amarouciaxanthin A (ACXA), exert similar functions in fibrogenesis pathways. In this study, we investigated the role of FCN and ACXA in the regulation of transforming growth factor β1 (TGFβ1)-induced pro-fibrogenic gene expression and oxidative stress in LX-2 cells, a human HSC cell line. TGFβ1 increased mRNA expression of pro-fibrogenic genes, such as α smooth muscle actin, procollagen type I α1 (COL1A1), COL3A1, and COL6A1, which FCN and ACXA significantly suppressed. Also, an increase in the protein level of COL1A1 by TGFβ1 was markedly decreased by ACXA. Furthermore, FCN and ACXA significantly inhibited TGFβ1-induced reactive oxygen species (ROS) accumulation with concomitantly decreasing antioxidant gene expression. Mechanistically, FCN and ACXA markedly increased TGFβ1-induced nuclear translocation of nuclear E2-related factor 2 (NRF2); however, they significantly attenuated TGFβ1-induced NFE2L2 and its target gene heme oxygenase-1 expression. The results suggest that FCN and ACXA exert anti-fibrogenic and antioxidant properties by inhibiting TGFβ1-induced pro-fibrogenic gene expression and ROS accumulation in LX-2 cells.
format	article
author	Yang Li Mi-Bo Kim Young-Ki Park Ji-Young Lee
author_facet	Yang Li Mi-Bo Kim Young-Ki Park Ji-Young Lee
author_sort	Yang Li
title	Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
title_short	Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
title_full	Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
title_fullStr	Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
title_full_unstemmed	Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
title_sort	fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e4deeafcca9745c5aad33f2fb3765696
work_keys_str_mv	AT yangli fucoxanthinmetabolitesexertantifibrogenicandantioxidanteffectsinhepaticstellatecells AT mibokim fucoxanthinmetabolitesexertantifibrogenicandantioxidanteffectsinhepaticstellatecells AT youngkipark fucoxanthinmetabolitesexertantifibrogenicandantioxidanteffectsinhepaticstellatecells AT jiyounglee fucoxanthinmetabolitesexertantifibrogenicandantioxidanteffectsinhepaticstellatecells
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Fucoxanthin metabolites exert anti-fibrogenic and antioxidant effects in hepatic stellate cells

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