Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth facto...

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Autores principales:	Félix-Antoine Pellerin, Christophe Caneparo, Ève Pellerin, Stéphane Chabaud, Martin Pelletier, Stéphane Bolduc
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	serum heat-inactivation 2D cell culture metabolism tissue engineering mechanical properties Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e4e19b56fbb745729f5457ef43acb8ba
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spelling	oai:doaj.org-article:e4e19b56fbb745729f5457ef43acb8ba2021-11-25T16:46:40ZHeat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts10.3390/bioengineering81101842306-5354https://doaj.org/article/e4e19b56fbb745729f5457ef43acb8ba2021-11-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/184https://doaj.org/toc/2306-5354Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth factors, that are very useful for cell culture? This is even truer in the case of tissue engineering, the processes of which is often very demanding. This balance is examined here, from nine populations of fibroblasts originating from three different organs, by comparing the capacity of adhesion and proliferation of cells, their metabolism, and the capacity to produce the stroma; their histological appearance, thickness, and mechanical properties were also evaluated. Overall, serum inactivation does not appear to provide a significant benefit.Félix-Antoine PellerinChristophe CaneparoÈve PellerinStéphane ChabaudMartin PelletierStéphane BolducMDPI AGarticleserumheat-inactivation2D cell culturemetabolismtissue engineeringmechanical propertiesTechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 184, p 184 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	serum heat-inactivation 2D cell culture metabolism tissue engineering mechanical properties Technology T Biology (General) QH301-705.5
spellingShingle	serum heat-inactivation 2D cell culture metabolism tissue engineering mechanical properties Technology T Biology (General) QH301-705.5 Félix-Antoine Pellerin Christophe Caneparo Ève Pellerin Stéphane Chabaud Martin Pelletier Stéphane Bolduc Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
description	Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth factors, that are very useful for cell culture? This is even truer in the case of tissue engineering, the processes of which is often very demanding. This balance is examined here, from nine populations of fibroblasts originating from three different organs, by comparing the capacity of adhesion and proliferation of cells, their metabolism, and the capacity to produce the stroma; their histological appearance, thickness, and mechanical properties were also evaluated. Overall, serum inactivation does not appear to provide a significant benefit.
format	article
author	Félix-Antoine Pellerin Christophe Caneparo Ève Pellerin Stéphane Chabaud Martin Pelletier Stéphane Bolduc
author_facet	Félix-Antoine Pellerin Christophe Caneparo Ève Pellerin Stéphane Chabaud Martin Pelletier Stéphane Bolduc
author_sort	Félix-Antoine Pellerin
title	Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
title_short	Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
title_full	Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
title_fullStr	Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
title_full_unstemmed	Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts
title_sort	heat-inactivation of fetal and newborn sera did not impair the expansion and scaffold engineering potentials of fibroblasts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e4e19b56fbb745729f5457ef43acb8ba
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Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

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