Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.

Fatty acid (FA) release from white adipose tissue (WAT) is the result of the balance between triglyceride breakdown and FA re-esterification. The latter relies on the induction of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), the key enzyme for glyceroneogenesis. We previously demonstrated...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anne-Marie Jaubert, Graziella Penot, Fatoumata Niang, Sylvie Durant, Claude Forest
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4e4bfd7ff0942d094cb6ea9af21a516
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e4e4bfd7ff0942d094cb6ea9af21a516
record_format	dspace
spelling	oai:doaj.org-article:e4e4bfd7ff0942d094cb6ea9af21a5162021-11-18T07:12:44ZRapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.1932-620310.1371/journal.pone.0040650https://doaj.org/article/e4e4bfd7ff0942d094cb6ea9af21a5162012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808220/?tool=EBIhttps://doaj.org/toc/1932-6203Fatty acid (FA) release from white adipose tissue (WAT) is the result of the balance between triglyceride breakdown and FA re-esterification. The latter relies on the induction of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), the key enzyme for glyceroneogenesis. We previously demonstrated that long-term (18 h) leptin treatment of rat epididymal WAT explants reduced glyceroneogenesis through nitric oxide (NO)-induced decrease in PEPCK-C expression. We investigated the effect of a short-term leptin treatment (2 h) on PEPCK-C expression and glyceroneogenesis in relation to NO production. We demonstrate that in WAT explants, leptin-induced NO synthase III (NOS III) phosphorylation was associated with reduced PEPCK-C level and glyceroneogenesis, leading to FA release, while PEPCK-C gene expression remained unaffected. These effects were absent in WAT explants from leptin receptor-deficient Zucker rat. Immunoprecipitation and western blot experiments showed that the leptin-induced decrease in PEPCK-C level was correlated with an increase in PEPCK-C nitration. All these effects were abolished by the NOS inhibitor Nω-nitro-L-arginine methyl ester and mimicked by the NO donor S-nitroso-N-acetyl-DL penicillamine. We propose a mechanism in which leptin activates NOS III and induces NO that nitrates PEPCK-C to reduce its level and glyceroneogenesis, therefore limiting FA re-esterification in WAT.Anne-Marie JaubertGraziella PenotFatoumata NiangSylvie DurantClaude ForestPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40650 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne-Marie Jaubert Graziella Penot Fatoumata Niang Sylvie Durant Claude Forest Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
description	Fatty acid (FA) release from white adipose tissue (WAT) is the result of the balance between triglyceride breakdown and FA re-esterification. The latter relies on the induction of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), the key enzyme for glyceroneogenesis. We previously demonstrated that long-term (18 h) leptin treatment of rat epididymal WAT explants reduced glyceroneogenesis through nitric oxide (NO)-induced decrease in PEPCK-C expression. We investigated the effect of a short-term leptin treatment (2 h) on PEPCK-C expression and glyceroneogenesis in relation to NO production. We demonstrate that in WAT explants, leptin-induced NO synthase III (NOS III) phosphorylation was associated with reduced PEPCK-C level and glyceroneogenesis, leading to FA release, while PEPCK-C gene expression remained unaffected. These effects were absent in WAT explants from leptin receptor-deficient Zucker rat. Immunoprecipitation and western blot experiments showed that the leptin-induced decrease in PEPCK-C level was correlated with an increase in PEPCK-C nitration. All these effects were abolished by the NOS inhibitor Nω-nitro-L-arginine methyl ester and mimicked by the NO donor S-nitroso-N-acetyl-DL penicillamine. We propose a mechanism in which leptin activates NOS III and induces NO that nitrates PEPCK-C to reduce its level and glyceroneogenesis, therefore limiting FA re-esterification in WAT.
format	article
author	Anne-Marie Jaubert Graziella Penot Fatoumata Niang Sylvie Durant Claude Forest
author_facet	Anne-Marie Jaubert Graziella Penot Fatoumata Niang Sylvie Durant Claude Forest
author_sort	Anne-Marie Jaubert
title	Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
title_short	Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
title_full	Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
title_fullStr	Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
title_full_unstemmed	Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
title_sort	rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e4e4bfd7ff0942d094cb6ea9af21a516
work_keys_str_mv	AT annemariejaubert rapidnitrationofadipocytephosphoenolpyruvatecarboxykinasebyleptinreducesglyceroneogenesisandinducesfattyacidrelease AT graziellapenot rapidnitrationofadipocytephosphoenolpyruvatecarboxykinasebyleptinreducesglyceroneogenesisandinducesfattyacidrelease AT fatoumataniang rapidnitrationofadipocytephosphoenolpyruvatecarboxykinasebyleptinreducesglyceroneogenesisandinducesfattyacidrelease AT sylviedurant rapidnitrationofadipocytephosphoenolpyruvatecarboxykinasebyleptinreducesglyceroneogenesisandinducesfattyacidrelease AT claudeforest rapidnitrationofadipocytephosphoenolpyruvatecarboxykinasebyleptinreducesglyceroneogenesisandinducesfattyacidrelease
_version_	1718423824735666176

Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.

Ejemplares similares