Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin

Abstract Background This study aimed to evaluate spexin as a novel blood marker and to describe the relationship of this peptide with selected biochemical metabolites measured during the transition period in dairy cows. Additionally, mRNA expression of the spexin gene as well as spexin receptors – g...

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Autores principales:	Robert Mikuła, Ewa Pruszyńska-Oszmałek, Marcin Pszczola, Justyna Rząsińska, Maciej Sassek, Krzysztof W. Nowak, Leszek Nogowski, Paweł A. Kołodziejski
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Dairy cow Transition period Energy balance Spexin Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/e4e76be0f8174b8b9366d1663ad86032
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spelling	oai:doaj.org-article:e4e76be0f8174b8b9366d1663ad860322021-11-21T12:25:52ZChanges in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin10.1186/s12917-021-03069-41746-6148https://doaj.org/article/e4e76be0f8174b8b9366d1663ad860322021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03069-4https://doaj.org/toc/1746-6148Abstract Background This study aimed to evaluate spexin as a novel blood marker and to describe the relationship of this peptide with selected biochemical metabolites measured during the transition period in dairy cows. Additionally, mRNA expression of the spexin gene as well as spexin receptors – galanin receptor type 2 and galanin receptor type 3, was investigated in several bovine tissues. Blood samples were collected at weekly intervals starting at 21 days before the estimated parturition day until 21 days in milk to determine concentrations of spexin, nonesterified fatty acids, β-hydroxybutyrate acid, total and active ghrelin, progesterone, glucose, insulin, IGF–I, triglycerides, cholesterol, leptin, corticosterone and 17-β-estradiol as well as the activity of aspartate transaminase, alkaline phosphatase and gamma-glutamyl transferase. Results Spexin concentration decreased from 21 d before parturition to calving day and next it rose during the first 14 d of lactation. The lowest concentration of spexin was recorded on the calving day and it differed from the mean level of this peptide before parturition as well as postpartum. Moreover, differences were observed between mean spexin concentrations before and after calving. Spexin levels were moderately negatively correlated with NEFA (r = − 0.39) and total ghrelin contents (r = − 0.41), weakly correlated with BHBA (r = − 0.35) while they showed a moderate positive relationship with progesterone concentrations (r = 0.42). Moreover, we detected that mRNA expression of GALR2, GALR3 and SPX is present in various bovine tissues (kidney, bowel, rumen, spinal cord, lung, skeletal muscle, liver, heart, fat and spleen). Conclusion A negative correlation between spexin concentration and NEFA, BHBA and total ghrelin contents as well as a positive relationship with levels of progesterone, metabolites and hormones, which are key players in the dairy cow transition period, may confirm an important function of this peptide in metabolism regulation. Thus measurement of spexin concentration could provide useful supplementary information for dairy cow herd health monitoring.Robert MikułaEwa Pruszyńska-OszmałekMarcin PszczolaJustyna RząsińskaMaciej SassekKrzysztof W. NowakLeszek NogowskiPaweł A. KołodziejskiBMCarticleDairy cowTransition periodEnergy balanceSpexinVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dairy cow Transition period Energy balance Spexin Veterinary medicine SF600-1100
spellingShingle	Dairy cow Transition period Energy balance Spexin Veterinary medicine SF600-1100 Robert Mikuła Ewa Pruszyńska-Oszmałek Marcin Pszczola Justyna Rząsińska Maciej Sassek Krzysztof W. Nowak Leszek Nogowski Paweł A. Kołodziejski Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
description	Abstract Background This study aimed to evaluate spexin as a novel blood marker and to describe the relationship of this peptide with selected biochemical metabolites measured during the transition period in dairy cows. Additionally, mRNA expression of the spexin gene as well as spexin receptors – galanin receptor type 2 and galanin receptor type 3, was investigated in several bovine tissues. Blood samples were collected at weekly intervals starting at 21 days before the estimated parturition day until 21 days in milk to determine concentrations of spexin, nonesterified fatty acids, β-hydroxybutyrate acid, total and active ghrelin, progesterone, glucose, insulin, IGF–I, triglycerides, cholesterol, leptin, corticosterone and 17-β-estradiol as well as the activity of aspartate transaminase, alkaline phosphatase and gamma-glutamyl transferase. Results Spexin concentration decreased from 21 d before parturition to calving day and next it rose during the first 14 d of lactation. The lowest concentration of spexin was recorded on the calving day and it differed from the mean level of this peptide before parturition as well as postpartum. Moreover, differences were observed between mean spexin concentrations before and after calving. Spexin levels were moderately negatively correlated with NEFA (r = − 0.39) and total ghrelin contents (r = − 0.41), weakly correlated with BHBA (r = − 0.35) while they showed a moderate positive relationship with progesterone concentrations (r = 0.42). Moreover, we detected that mRNA expression of GALR2, GALR3 and SPX is present in various bovine tissues (kidney, bowel, rumen, spinal cord, lung, skeletal muscle, liver, heart, fat and spleen). Conclusion A negative correlation between spexin concentration and NEFA, BHBA and total ghrelin contents as well as a positive relationship with levels of progesterone, metabolites and hormones, which are key players in the dairy cow transition period, may confirm an important function of this peptide in metabolism regulation. Thus measurement of spexin concentration could provide useful supplementary information for dairy cow herd health monitoring.
format	article
author	Robert Mikuła Ewa Pruszyńska-Oszmałek Marcin Pszczola Justyna Rząsińska Maciej Sassek Krzysztof W. Nowak Leszek Nogowski Paweł A. Kołodziejski
author_facet	Robert Mikuła Ewa Pruszyńska-Oszmałek Marcin Pszczola Justyna Rząsińska Maciej Sassek Krzysztof W. Nowak Leszek Nogowski Paweł A. Kołodziejski
author_sort	Robert Mikuła
title	Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
title_short	Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
title_full	Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
title_fullStr	Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
title_full_unstemmed	Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
title_sort	changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin
publisher	BMC
publishDate	2021
url	https://doaj.org/article/e4e76be0f8174b8b9366d1663ad86032
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Changes in metabolic and hormonal profiles during transition period in dairy cattle – the role of spexin

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