Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect

Hot-band absorption (HBA)-induced anti-Stokes fluorescence (ASF) with longer-wavelength excitation is one effective pathway to deep penetration and low autofluorescence in intravital fluorescence imaging, raising demands for fluorophores with broad spectra, high absorption, and strong emission. Howe...

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Autores principales:	Yuhuang Zhang, Jing Zhou, Shiyi Peng, Wenbin Yu, Xiaoxiao Fan, Wen Liu, Zikang Ye, Ji Qi, Zhe Feng, Jun Qian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anti-Stokes fluorescence aggregation-induced emission hot-band absorption in vivo confocal imaging multi-photon microscopy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e4f186765a734fb0952f3c243a9b3d45
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spelling	oai:doaj.org-article:e4f186765a734fb0952f3c243a9b3d452021-11-25T16:55:53ZHot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect10.3390/bios111104682079-6374https://doaj.org/article/e4f186765a734fb0952f3c243a9b3d452021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/468https://doaj.org/toc/2079-6374Hot-band absorption (HBA)-induced anti-Stokes fluorescence (ASF) with longer-wavelength excitation is one effective pathway to deep penetration and low autofluorescence in intravital fluorescence imaging, raising demands for fluorophores with broad spectra, high absorption, and strong emission. However, typical fluorescent dyes display some emission quenching when their concentration is increased in order to obtain brighter fluorescence. In this work, the HBA-induced ASF of aggregation-induced emission (AIE) dots is reported. BPN-BBTD dots were synthesized and confirmed with a fluorescence enhancement and a considerable ASF intensity. In addition, the mechanism of ASF and the HBA process of BPN-BBTD dots were carefully validated and discussed. To obtain the full advantages of the long-wavelength excitation and the short fluorescence lifetime in deep-tissue bioimaging, a large-depth ASF confocal microscopic imaging of in vivo cerebral vasculature was conducted under the excitation of a 980 nm continuous wave laser after intravenous injection of BPN-BBTD dots. Meanwhile, the 3D structure of the cerebrovascular network was successfully reconstructed.Yuhuang ZhangJing ZhouShiyi PengWenbin YuXiaoxiao FanWen LiuZikang YeJi QiZhe FengJun QianMDPI AGarticleanti-Stokes fluorescenceaggregation-induced emissionhot-band absorptionin vivo confocal imagingmulti-photon microscopyBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 468, p 468 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-Stokes fluorescence aggregation-induced emission hot-band absorption in vivo confocal imaging multi-photon microscopy Biotechnology TP248.13-248.65
spellingShingle	anti-Stokes fluorescence aggregation-induced emission hot-band absorption in vivo confocal imaging multi-photon microscopy Biotechnology TP248.13-248.65 Yuhuang Zhang Jing Zhou Shiyi Peng Wenbin Yu Xiaoxiao Fan Wen Liu Zikang Ye Ji Qi Zhe Feng Jun Qian Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
description	Hot-band absorption (HBA)-induced anti-Stokes fluorescence (ASF) with longer-wavelength excitation is one effective pathway to deep penetration and low autofluorescence in intravital fluorescence imaging, raising demands for fluorophores with broad spectra, high absorption, and strong emission. However, typical fluorescent dyes display some emission quenching when their concentration is increased in order to obtain brighter fluorescence. In this work, the HBA-induced ASF of aggregation-induced emission (AIE) dots is reported. BPN-BBTD dots were synthesized and confirmed with a fluorescence enhancement and a considerable ASF intensity. In addition, the mechanism of ASF and the HBA process of BPN-BBTD dots were carefully validated and discussed. To obtain the full advantages of the long-wavelength excitation and the short fluorescence lifetime in deep-tissue bioimaging, a large-depth ASF confocal microscopic imaging of in vivo cerebral vasculature was conducted under the excitation of a 980 nm continuous wave laser after intravenous injection of BPN-BBTD dots. Meanwhile, the 3D structure of the cerebrovascular network was successfully reconstructed.
format	article
author	Yuhuang Zhang Jing Zhou Shiyi Peng Wenbin Yu Xiaoxiao Fan Wen Liu Zikang Ye Ji Qi Zhe Feng Jun Qian
author_facet	Yuhuang Zhang Jing Zhou Shiyi Peng Wenbin Yu Xiaoxiao Fan Wen Liu Zikang Ye Ji Qi Zhe Feng Jun Qian
author_sort	Yuhuang Zhang
title	Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
title_short	Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
title_full	Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
title_fullStr	Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
title_full_unstemmed	Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
title_sort	hot-band-absorption-induced anti-stokes fluorescence of aggregation-induced emission dots and the influence on the nonlinear optical effect
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e4f186765a734fb0952f3c243a9b3d45
work_keys_str_mv	AT yuhuangzhang hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT jingzhou hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT shiyipeng hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT wenbinyu hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT xiaoxiaofan hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT wenliu hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT zikangye hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT jiqi hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT zhefeng hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect AT junqian hotbandabsorptioninducedantistokesfluorescenceofaggregationinducedemissiondotsandtheinfluenceonthenonlinearopticaleffect
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Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect

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