Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.

<h4>Background</h4>Interferon-induced 35-kDa protein (IFP35) plays important roles in antiviral defense and the progression of some skin cancer diseases. It can be induced by interferon-γ (IFN-γ) in multiple human cells. However, the mechanisms by which IFN-γ contributes to IFP35 inducti...

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Autores principales:	Wei Yang, Juan Tan, Ruikang Liu, Xiaoxu Cui, Qinglin Ma, Yunqi Geng, Wentao Qiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e50235949dbe49249ef9587263387088
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spelling	oai:doaj.org-article:e50235949dbe49249ef95872633870882021-11-18T08:06:25ZInterferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.1932-620310.1371/journal.pone.0050932https://doaj.org/article/e50235949dbe49249ef95872633870882012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226549/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Interferon-induced 35-kDa protein (IFP35) plays important roles in antiviral defense and the progression of some skin cancer diseases. It can be induced by interferon-γ (IFN-γ) in multiple human cells. However, the mechanisms by which IFN-γ contributes to IFP35 induction remain to be elucidated.<h4>Methods/principal findings</h4>We identified the transcription start sites of IFP35 by 5' rapid amplification of cDNA ends (RACE) and cloned the promoter of IFP35. Sequence analysis and luciferase assays revealed two GC boxes and an IFN-stimulated response element (ISRE) in the 5' upstream region of the transcription start sites, which were important for the basal transcription of IFP35 gene. Furthermore, we found that interferon regulatory factor 1 (IRF-1) and IRF-2 could bind to IFP35 promoter and upregulate endogenous IFP35 protein level. Depletion of endogenous IRF-1 by interfering RNA reduced the constitutive and IFN-γ-dependent expression of IFP35, whereas depletion of IRF-2 had little effect on IFN-γ-inducible IFP35 expression. Moreover, IRF-1 was recruited to the ISRE site in IFP35 promoter in IFN-γ treated HeLa cells, as demonstrated by electrophoretic mobility shift and chromatin immunoprecipitation assays.<h4>Conclusions/significance</h4>These findings provide the first evidence that IRF-1 and IRF-2 are involved in constitutive IFP35 expression in HeLa cells, while IRF-1 also activates IFP35 expression in an IFN-γ-inducible manner. Our data therefore identified a new IRF-1 and IRF-2 target gene, which may expand our current understanding of the versatile functions of IRF-1 and IRF-2.Wei YangJuan TanRuikang LiuXiaoxu CuiQinglin MaYunqi GengWentao QiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e50932 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Yang Juan Tan Ruikang Liu Xiaoxu Cui Qinglin Ma Yunqi Geng Wentao Qiao Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
description	<h4>Background</h4>Interferon-induced 35-kDa protein (IFP35) plays important roles in antiviral defense and the progression of some skin cancer diseases. It can be induced by interferon-γ (IFN-γ) in multiple human cells. However, the mechanisms by which IFN-γ contributes to IFP35 induction remain to be elucidated.<h4>Methods/principal findings</h4>We identified the transcription start sites of IFP35 by 5' rapid amplification of cDNA ends (RACE) and cloned the promoter of IFP35. Sequence analysis and luciferase assays revealed two GC boxes and an IFN-stimulated response element (ISRE) in the 5' upstream region of the transcription start sites, which were important for the basal transcription of IFP35 gene. Furthermore, we found that interferon regulatory factor 1 (IRF-1) and IRF-2 could bind to IFP35 promoter and upregulate endogenous IFP35 protein level. Depletion of endogenous IRF-1 by interfering RNA reduced the constitutive and IFN-γ-dependent expression of IFP35, whereas depletion of IRF-2 had little effect on IFN-γ-inducible IFP35 expression. Moreover, IRF-1 was recruited to the ISRE site in IFP35 promoter in IFN-γ treated HeLa cells, as demonstrated by electrophoretic mobility shift and chromatin immunoprecipitation assays.<h4>Conclusions/significance</h4>These findings provide the first evidence that IRF-1 and IRF-2 are involved in constitutive IFP35 expression in HeLa cells, while IRF-1 also activates IFP35 expression in an IFN-γ-inducible manner. Our data therefore identified a new IRF-1 and IRF-2 target gene, which may expand our current understanding of the versatile functions of IRF-1 and IRF-2.
format	article
author	Wei Yang Juan Tan Ruikang Liu Xiaoxu Cui Qinglin Ma Yunqi Geng Wentao Qiao
author_facet	Wei Yang Juan Tan Ruikang Liu Xiaoxu Cui Qinglin Ma Yunqi Geng Wentao Qiao
author_sort	Wei Yang
title	Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
title_short	Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
title_full	Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
title_fullStr	Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
title_full_unstemmed	Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.
title_sort	interferon-γ upregulates expression of ifp35 gene in hela cells via interferon regulatory factor-1.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e50235949dbe49249ef9587263387088
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Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.

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