Single-Cell Transcriptomics Analysis of Human Small Antral Follicles

Human ovarian folliculogenesis is a highly regulated and complex process. Characterization of follicular cell signatures during this dynamic process is important to understand follicle fate (to grow, become dominant, or undergo atresia). The transcriptional signature of human oocytes and granulosa c...

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Autores principales:	Xueying Fan, Ioannis Moustakas, Monika Bialecka, Julieta S. del Valle, Arend W. Overeem, Leoni A. Louwe, Gonneke S. K. Pilgram, Lucette A. J. van der Westerlaken, Hailiang Mei, Susana M. Chuva de Sousa Lopes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	human adult ovary oocyte granulosa cell antral follicle single-cell transcriptomics signaling pathways Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e505a4facbd64435b1cd0d038ad3c603
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spelling	oai:doaj.org-article:e505a4facbd64435b1cd0d038ad3c6032021-11-11T17:22:36ZSingle-Cell Transcriptomics Analysis of Human Small Antral Follicles10.3390/ijms2221119551422-00671661-6596https://doaj.org/article/e505a4facbd64435b1cd0d038ad3c6032021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11955https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Human ovarian folliculogenesis is a highly regulated and complex process. Characterization of follicular cell signatures during this dynamic process is important to understand follicle fate (to grow, become dominant, or undergo atresia). The transcriptional signature of human oocytes and granulosa cells (GCs) in early-growing and ovulatory follicles have been previously described; however, that of oocytes with surrounding GCs in small antral follicles have not been studied yet. Here, we have generated a unique dataset of single-cell transcriptomics (SmartSeq2) consisting of the oocyte with surrounding GCs from several individual (non-dominant) small antral follicles isolated from adult human ovaries. We have identified two main types of (healthy) follicles, with a distinct oocyte and GC signature. Using the CellphoneDB algorithm, we then investigated the bi-directional ligand–receptor interactions regarding the transforming growth factor-β (TGFβ)/bone morphogenetic protein (BMP), wingless-type (MMTV)-integration site (WNT), NOTCH, and receptor tyrosine kinases (RTK) signaling pathways between oocyte and GCs within each antral follicle type. Our work not only revealed the diversity of small antral follicles, but also contributes to fill the gap in mapping the molecular landscape of human folliculogenesis and oogenesis.Xueying FanIoannis MoustakasMonika BialeckaJulieta S. del ValleArend W. OvereemLeoni A. LouweGonneke S. K. PilgramLucette A. J. van der WesterlakenHailiang MeiSusana M. Chuva de Sousa LopesMDPI AGarticlehuman adult ovaryoocytegranulosa cellantral folliclesingle-cell transcriptomicssignaling pathwaysBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11955, p 11955 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human adult ovary oocyte granulosa cell antral follicle single-cell transcriptomics signaling pathways Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	human adult ovary oocyte granulosa cell antral follicle single-cell transcriptomics signaling pathways Biology (General) QH301-705.5 Chemistry QD1-999 Xueying Fan Ioannis Moustakas Monika Bialecka Julieta S. del Valle Arend W. Overeem Leoni A. Louwe Gonneke S. K. Pilgram Lucette A. J. van der Westerlaken Hailiang Mei Susana M. Chuva de Sousa Lopes Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
description	Human ovarian folliculogenesis is a highly regulated and complex process. Characterization of follicular cell signatures during this dynamic process is important to understand follicle fate (to grow, become dominant, or undergo atresia). The transcriptional signature of human oocytes and granulosa cells (GCs) in early-growing and ovulatory follicles have been previously described; however, that of oocytes with surrounding GCs in small antral follicles have not been studied yet. Here, we have generated a unique dataset of single-cell transcriptomics (SmartSeq2) consisting of the oocyte with surrounding GCs from several individual (non-dominant) small antral follicles isolated from adult human ovaries. We have identified two main types of (healthy) follicles, with a distinct oocyte and GC signature. Using the CellphoneDB algorithm, we then investigated the bi-directional ligand–receptor interactions regarding the transforming growth factor-β (TGFβ)/bone morphogenetic protein (BMP), wingless-type (MMTV)-integration site (WNT), NOTCH, and receptor tyrosine kinases (RTK) signaling pathways between oocyte and GCs within each antral follicle type. Our work not only revealed the diversity of small antral follicles, but also contributes to fill the gap in mapping the molecular landscape of human folliculogenesis and oogenesis.
format	article
author	Xueying Fan Ioannis Moustakas Monika Bialecka Julieta S. del Valle Arend W. Overeem Leoni A. Louwe Gonneke S. K. Pilgram Lucette A. J. van der Westerlaken Hailiang Mei Susana M. Chuva de Sousa Lopes
author_facet	Xueying Fan Ioannis Moustakas Monika Bialecka Julieta S. del Valle Arend W. Overeem Leoni A. Louwe Gonneke S. K. Pilgram Lucette A. J. van der Westerlaken Hailiang Mei Susana M. Chuva de Sousa Lopes
author_sort	Xueying Fan
title	Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
title_short	Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
title_full	Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
title_fullStr	Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
title_full_unstemmed	Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
title_sort	single-cell transcriptomics analysis of human small antral follicles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e505a4facbd64435b1cd0d038ad3c603
work_keys_str_mv	AT xueyingfan singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT ioannismoustakas singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT monikabialecka singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT julietasdelvalle singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT arendwovereem singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT leonialouwe singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT gonnekeskpilgram singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT lucetteajvanderwesterlaken singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT hailiangmei singlecelltranscriptomicsanalysisofhumansmallantralfollicles AT susanamchuvadesousalopes singlecelltranscriptomicsanalysisofhumansmallantralfollicles
_version_	1718432151367581696

Single-Cell Transcriptomics Analysis of Human Small Antral Follicles

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