Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing

Abstract The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present s...

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Autores principales:	Xiang-dong Zi, Jian-yuan Lu, Li Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e50ef764501046778603339a6eeb3de3
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spelling	oai:doaj.org-article:e50ef764501046778603339a6eeb3de32021-12-02T16:06:14ZIdentification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing10.1038/s41598-017-02225-x2045-2322https://doaj.org/article/e50ef764501046778603339a6eeb3de32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02225-xhttps://doaj.org/toc/2045-2322Abstract The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present study provides the first comparison of the ovarian miRNAs of prolific Jintang black goats (JTGs) and non-prolific Tibetan goats (TBGs) during the follicular phase using RNA-Seq technology. We generated 11.19 million (M) and 11.34 M clean reads from the TBG and JTG libraries, respectively, from which a total of 389 known miRNAs were identified and 142 novel miRNAs were predicted. A total of 191 miRNAs were differentially expressed between the two breeds. Among the 10 most abundant miRNAs, miR-21-5p was defined as differentially expressed miRNA with a higher level in the JTG library than in the TBG library, but the other miRNAs were not different between the breeds. The predicted miRNA-targeted genes were further analyzed by Gene Ontology and KEGG pathway analyses. The results revealed that miR-21, miR-99a, miRNA-143, let-7f, miR-493 and miR-200b may affect follicular development. These findings will increase the current understanding of the role of ovarian miRNAs in the regulation of ovulation rate in goats.Xiang-dong ZiJian-yuan LuLi MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiang-dong Zi Jian-yuan Lu Li Ma Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
description	Abstract The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present study provides the first comparison of the ovarian miRNAs of prolific Jintang black goats (JTGs) and non-prolific Tibetan goats (TBGs) during the follicular phase using RNA-Seq technology. We generated 11.19 million (M) and 11.34 M clean reads from the TBG and JTG libraries, respectively, from which a total of 389 known miRNAs were identified and 142 novel miRNAs were predicted. A total of 191 miRNAs were differentially expressed between the two breeds. Among the 10 most abundant miRNAs, miR-21-5p was defined as differentially expressed miRNA with a higher level in the JTG library than in the TBG library, but the other miRNAs were not different between the breeds. The predicted miRNA-targeted genes were further analyzed by Gene Ontology and KEGG pathway analyses. The results revealed that miR-21, miR-99a, miRNA-143, let-7f, miR-493 and miR-200b may affect follicular development. These findings will increase the current understanding of the role of ovarian miRNAs in the regulation of ovulation rate in goats.
format	article
author	Xiang-dong Zi Jian-yuan Lu Li Ma
author_facet	Xiang-dong Zi Jian-yuan Lu Li Ma
author_sort	Xiang-dong Zi
title	Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
title_short	Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
title_full	Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
title_fullStr	Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
title_full_unstemmed	Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
title_sort	identification and comparative analysis of the ovarian micrornas of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e50ef764501046778603339a6eeb3de3
work_keys_str_mv	AT xiangdongzi identificationandcomparativeanalysisoftheovarianmicrornasofprolificandnonprolificgoatsduringthefollicularphaseusinghighthroughputsequencing AT jianyuanlu identificationandcomparativeanalysisoftheovarianmicrornasofprolificandnonprolificgoatsduringthefollicularphaseusinghighthroughputsequencing AT lima identificationandcomparativeanalysisoftheovarianmicrornasofprolificandnonprolificgoatsduringthefollicularphaseusinghighthroughputsequencing
_version_	1718385062427230208

Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing

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