Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii

Single-stranded oligodeoxynucleotides are often used as templates for DNA repair during genome editing. Here the authors show that, unlike in animals, single-strand templated DNA repair in Chlamydomonas reinhardtii relies on the alternative end-joining enzyme polymerase θ.

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Autores principales:	Aron Ferenczi, Yen Peng Chew, Erika Kroll, Charlotte von Koppenfels, Andrew Hudson, Attila Molnar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e51404c28f9b46dcacd8592ecab3a808
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spelling	oai:doaj.org-article:e51404c28f9b46dcacd8592ecab3a8082021-11-21T12:33:56ZMechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii10.1038/s41467-021-27004-12041-1723https://doaj.org/article/e51404c28f9b46dcacd8592ecab3a8082021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27004-1https://doaj.org/toc/2041-1723Single-stranded oligodeoxynucleotides are often used as templates for DNA repair during genome editing. Here the authors show that, unlike in animals, single-strand templated DNA repair in Chlamydomonas reinhardtii relies on the alternative end-joining enzyme polymerase θ.Aron FerencziYen Peng ChewErika KrollCharlotte von KoppenfelsAndrew HudsonAttila MolnarNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Aron Ferenczi Yen Peng Chew Erika Kroll Charlotte von Koppenfels Andrew Hudson Attila Molnar Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
description	Single-stranded oligodeoxynucleotides are often used as templates for DNA repair during genome editing. Here the authors show that, unlike in animals, single-strand templated DNA repair in Chlamydomonas reinhardtii relies on the alternative end-joining enzyme polymerase θ.
format	article
author	Aron Ferenczi Yen Peng Chew Erika Kroll Charlotte von Koppenfels Andrew Hudson Attila Molnar
author_facet	Aron Ferenczi Yen Peng Chew Erika Kroll Charlotte von Koppenfels Andrew Hudson Attila Molnar
author_sort	Aron Ferenczi
title	Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
title_short	Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
title_full	Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
title_fullStr	Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
title_full_unstemmed	Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
title_sort	mechanistic and genetic basis of single-strand templated repair at cas12a-induced dna breaks in chlamydomonas reinhardtii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e51404c28f9b46dcacd8592ecab3a808
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Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii

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