CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogen...

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Autores principales:	Jun Wu, Marcela Vilarino, Keiichiro Suzuki, Daiji Okamura, Yanina Soledad Bogliotti, Insung Park, Joan Rowe, Bret McNabb, Pablo Juan Ross, Juan Carlos Izpisua Belmonte
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5179872561d480fb6dfc184638202f4
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spelling	oai:doaj.org-article:e5179872561d480fb6dfc184638202f42021-12-02T15:06:15ZCRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs10.1038/s41598-017-08596-52045-2322https://doaj.org/article/e5179872561d480fb6dfc184638202f42017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08596-5https://doaj.org/toc/2045-2322Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.Jun WuMarcela VilarinoKeiichiro SuzukiDaiji OkamuraYanina Soledad BogliottiInsung ParkJoan RoweBret McNabbPablo Juan RossJuan Carlos Izpisua BelmonteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
description	Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.
format	article
author	Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte
author_facet	Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte
author_sort	Jun Wu
title	CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_short	CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_full	CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_fullStr	CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_full_unstemmed	CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_sort	crispr-cas9 mediated one-step disabling of pancreatogenesis in pigs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e5179872561d480fb6dfc184638202f4
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_version_	1718388550932627456

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

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