Ecological quality in freshwater streams is reflected across all three domains of life

Assessment of ecological quality in streaming surface water is often based on different Biological Quality Elements (BQE) such as plants, fish or invertebrates. Conventional stream-water quality assessment based on invertebrates as BQE relies on taxonomic expertise, which is costly and time consumin...

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Autores principales:	Nadieh de Jonge, Franziska Kuntke, Martin Hesselsøe, Jeppe Lund Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Ecological quality Water stream quality Metabarcoding Bacteria Archaea Eukaryotes Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/e5182fb784a24ebbaaf38b3ff3791dda
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id	oai:doaj.org-article:e5182fb784a24ebbaaf38b3ff3791dda
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spelling	oai:doaj.org-article:e5182fb784a24ebbaaf38b3ff3791dda2021-12-01T04:58:38ZEcological quality in freshwater streams is reflected across all three domains of life1470-160X10.1016/j.ecolind.2021.108059https://doaj.org/article/e5182fb784a24ebbaaf38b3ff3791dda2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X2100724Xhttps://doaj.org/toc/1470-160XAssessment of ecological quality in streaming surface water is often based on different Biological Quality Elements (BQE) such as plants, fish or invertebrates. Conventional stream-water quality assessment based on invertebrates as BQE relies on taxonomic expertise, which is costly and time consuming. Next-generation sequencing approaches for high-throughput analyses of diverse ecosystems are increasingly used for environmental monitoring and holds a great potential for application in stream-water quality assessments. This approach is to some extent hampered by the currently available reference databases representing freshwater invertebrates. In the present study we apply metabarcoding simultaneously targeting the 16S (prokaryotes) and 18S (eukaryotes) rRNA genes to capture a snapshot of the ecosystem composition across the three domains of life. Results based on the analysis of 50 selected Danish streams showed that the combined, as well as the domain-specific profiles can separate the samples into their respective ecological quality categories as reflected by the parallel conventional assessment based on macroinvertebrates as BQE. Furthermore, it was possible to suggest potential indicator organisms, from all three domains, which correlated specifically to the conventional data e.g. organisms with a strong correlation to ecological status across all categories. The results clearly showed that community structure in all three domains of life reflect the ecological status of the sample location. Hence, when applying a molecular approach for water-quality assessment we are not limited to the composition of visible BQE, such as macroinvertebrates. The microbial community composition in the streams may often capture an even better and more comprehensive and sensitive snapshot of the ecological quality of stream waters.Nadieh de JongeFranziska KuntkeMartin HesselsøeJeppe Lund NielsenElsevierarticleEcological qualityWater stream qualityMetabarcodingBacteriaArchaeaEukaryotesEcologyQH540-549.5ENEcological Indicators, Vol 130, Iss , Pp 108059- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ecological quality Water stream quality Metabarcoding Bacteria Archaea Eukaryotes Ecology QH540-549.5
spellingShingle	Ecological quality Water stream quality Metabarcoding Bacteria Archaea Eukaryotes Ecology QH540-549.5 Nadieh de Jonge Franziska Kuntke Martin Hesselsøe Jeppe Lund Nielsen Ecological quality in freshwater streams is reflected across all three domains of life
description	Assessment of ecological quality in streaming surface water is often based on different Biological Quality Elements (BQE) such as plants, fish or invertebrates. Conventional stream-water quality assessment based on invertebrates as BQE relies on taxonomic expertise, which is costly and time consuming. Next-generation sequencing approaches for high-throughput analyses of diverse ecosystems are increasingly used for environmental monitoring and holds a great potential for application in stream-water quality assessments. This approach is to some extent hampered by the currently available reference databases representing freshwater invertebrates. In the present study we apply metabarcoding simultaneously targeting the 16S (prokaryotes) and 18S (eukaryotes) rRNA genes to capture a snapshot of the ecosystem composition across the three domains of life. Results based on the analysis of 50 selected Danish streams showed that the combined, as well as the domain-specific profiles can separate the samples into their respective ecological quality categories as reflected by the parallel conventional assessment based on macroinvertebrates as BQE. Furthermore, it was possible to suggest potential indicator organisms, from all three domains, which correlated specifically to the conventional data e.g. organisms with a strong correlation to ecological status across all categories. The results clearly showed that community structure in all three domains of life reflect the ecological status of the sample location. Hence, when applying a molecular approach for water-quality assessment we are not limited to the composition of visible BQE, such as macroinvertebrates. The microbial community composition in the streams may often capture an even better and more comprehensive and sensitive snapshot of the ecological quality of stream waters.
format	article
author	Nadieh de Jonge Franziska Kuntke Martin Hesselsøe Jeppe Lund Nielsen
author_facet	Nadieh de Jonge Franziska Kuntke Martin Hesselsøe Jeppe Lund Nielsen
author_sort	Nadieh de Jonge
title	Ecological quality in freshwater streams is reflected across all three domains of life
title_short	Ecological quality in freshwater streams is reflected across all three domains of life
title_full	Ecological quality in freshwater streams is reflected across all three domains of life
title_fullStr	Ecological quality in freshwater streams is reflected across all three domains of life
title_full_unstemmed	Ecological quality in freshwater streams is reflected across all three domains of life
title_sort	ecological quality in freshwater streams is reflected across all three domains of life
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e5182fb784a24ebbaaf38b3ff3791dda
work_keys_str_mv	AT nadiehdejonge ecologicalqualityinfreshwaterstreamsisreflectedacrossallthreedomainsoflife AT franziskakuntke ecologicalqualityinfreshwaterstreamsisreflectedacrossallthreedomainsoflife AT martinhesselsøe ecologicalqualityinfreshwaterstreamsisreflectedacrossallthreedomainsoflife AT jeppelundnielsen ecologicalqualityinfreshwaterstreamsisreflectedacrossallthreedomainsoflife
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Ecological quality in freshwater streams is reflected across all three domains of life

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