The integration of Gβ and MAPK signaling cascade in zygote development

Abstract Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is in...

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Autores principales:	Guo-Liang Yuan, Hong-Ju Li, Wei-Cai Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e51bc325405347b586ad9dc9d0b043c3
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spelling	oai:doaj.org-article:e51bc325405347b586ad9dc9d0b043c32021-12-02T15:05:23ZThe integration of Gβ and MAPK signaling cascade in zygote development10.1038/s41598-017-08230-42045-2322https://doaj.org/article/e51bc325405347b586ad9dc9d0b043c32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08230-4https://doaj.org/toc/2045-2322Abstract Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is integrated with G proteins by scaffold protein during plant immunity. However, the molecular relationship between G proteins and MAPK modules in plant development is still unclear. In this study, we demonstrate that Arabidopsis Gβ protein AGB1 interacts with MPK3 and 6, MKK4 and 5, as well as the regulatory domains of YODA (YDA), the upstream MEKK of MKK4/5. Remarkably, YDA interacts with the plasma membrane associated SHORT SUSPENSOR (SSP) through its N- and C-terminal region in vitro and in vivo. Additionally, genetic analysis shows that AGB1 functions together with MPK3/6 signaling cascade during the asymmetric division of the zygote. These data indicate that Gβ may function likely as a scaffold, through direct physical interaction with the components of the MPK signaling module in plant development. Our results provide new insights into the molecular functions of G protein and will advance the understanding of the complex mechanism of kinase signaling cascades.Guo-Liang YuanHong-Ju LiWei-Cai YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guo-Liang Yuan Hong-Ju Li Wei-Cai Yang The integration of Gβ and MAPK signaling cascade in zygote development
description	Abstract Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is integrated with G proteins by scaffold protein during plant immunity. However, the molecular relationship between G proteins and MAPK modules in plant development is still unclear. In this study, we demonstrate that Arabidopsis Gβ protein AGB1 interacts with MPK3 and 6, MKK4 and 5, as well as the regulatory domains of YODA (YDA), the upstream MEKK of MKK4/5. Remarkably, YDA interacts with the plasma membrane associated SHORT SUSPENSOR (SSP) through its N- and C-terminal region in vitro and in vivo. Additionally, genetic analysis shows that AGB1 functions together with MPK3/6 signaling cascade during the asymmetric division of the zygote. These data indicate that Gβ may function likely as a scaffold, through direct physical interaction with the components of the MPK signaling module in plant development. Our results provide new insights into the molecular functions of G protein and will advance the understanding of the complex mechanism of kinase signaling cascades.
format	article
author	Guo-Liang Yuan Hong-Ju Li Wei-Cai Yang
author_facet	Guo-Liang Yuan Hong-Ju Li Wei-Cai Yang
author_sort	Guo-Liang Yuan
title	The integration of Gβ and MAPK signaling cascade in zygote development
title_short	The integration of Gβ and MAPK signaling cascade in zygote development
title_full	The integration of Gβ and MAPK signaling cascade in zygote development
title_fullStr	The integration of Gβ and MAPK signaling cascade in zygote development
title_full_unstemmed	The integration of Gβ and MAPK signaling cascade in zygote development
title_sort	integration of gβ and mapk signaling cascade in zygote development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e51bc325405347b586ad9dc9d0b043c3
work_keys_str_mv	AT guoliangyuan theintegrationofgbandmapksignalingcascadeinzygotedevelopment AT hongjuli theintegrationofgbandmapksignalingcascadeinzygotedevelopment AT weicaiyang theintegrationofgbandmapksignalingcascadeinzygotedevelopment AT guoliangyuan integrationofgbandmapksignalingcascadeinzygotedevelopment AT hongjuli integrationofgbandmapksignalingcascadeinzygotedevelopment AT weicaiyang integrationofgbandmapksignalingcascadeinzygotedevelopment
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The integration of Gβ and MAPK signaling cascade in zygote development

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