DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia

Abstract The morphogenic factor Sonic hedgehog (Shh) signals through the primary cilium, which relies on intraflagellar transport to maintain its structural integrity and function. However, the process by which protein and lipid cargos are delivered to the primary cilium from their sites of synthesi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jie Ding, Lei Shao, Yixing Yao, Xin Tong, Huaize Liu, Shen Yue, Lu Xie, Steven Y. Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5210e7b77f24980b5cd040dcfcd662c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e5210e7b77f24980b5cd040dcfcd662c
record_format	dspace
spelling	oai:doaj.org-article:e5210e7b77f24980b5cd040dcfcd662c2021-12-02T11:40:20ZDGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia10.1038/s41598-017-05680-82045-2322https://doaj.org/article/e5210e7b77f24980b5cd040dcfcd662c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05680-8https://doaj.org/toc/2045-2322Abstract The morphogenic factor Sonic hedgehog (Shh) signals through the primary cilium, which relies on intraflagellar transport to maintain its structural integrity and function. However, the process by which protein and lipid cargos are delivered to the primary cilium from their sites of synthesis still remains poorly characterized. Here, we report that diacylglycerol kinase δ (DGKδ), a residential lipid kinase in the endoplasmic reticulum, triggers the release of IFT88-containing vesicles from the ER exit sites (ERES), thereby setting forth their movement to the primary cilium. Encoded by the gene whose mutations originally implicated the primary cilium as the venue of Shh signaling, IFT88 is known to be part of the complex B that drives the anterograde transport within cilia. We show that IFT88 interacts with DGKδ, and is associated with COPII-coated vesicles at the ERES. Using a combination of RNAi silencing and gene knockout strategies, we further show that DGKδ is required for supporting Shh signaling both in vitro and in vivo, demonstrating the physiological significance of this regulation.Jie DingLei ShaoYixing YaoXin TongHuaize LiuShen YueLu XieSteven Y. ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie Ding Lei Shao Yixing Yao Xin Tong Huaize Liu Shen Yue Lu Xie Steven Y. Cheng DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
description	Abstract The morphogenic factor Sonic hedgehog (Shh) signals through the primary cilium, which relies on intraflagellar transport to maintain its structural integrity and function. However, the process by which protein and lipid cargos are delivered to the primary cilium from their sites of synthesis still remains poorly characterized. Here, we report that diacylglycerol kinase δ (DGKδ), a residential lipid kinase in the endoplasmic reticulum, triggers the release of IFT88-containing vesicles from the ER exit sites (ERES), thereby setting forth their movement to the primary cilium. Encoded by the gene whose mutations originally implicated the primary cilium as the venue of Shh signaling, IFT88 is known to be part of the complex B that drives the anterograde transport within cilia. We show that IFT88 interacts with DGKδ, and is associated with COPII-coated vesicles at the ERES. Using a combination of RNAi silencing and gene knockout strategies, we further show that DGKδ is required for supporting Shh signaling both in vitro and in vivo, demonstrating the physiological significance of this regulation.
format	article
author	Jie Ding Lei Shao Yixing Yao Xin Tong Huaize Liu Shen Yue Lu Xie Steven Y. Cheng
author_facet	Jie Ding Lei Shao Yixing Yao Xin Tong Huaize Liu Shen Yue Lu Xie Steven Y. Cheng
author_sort	Jie Ding
title	DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
title_short	DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
title_full	DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
title_fullStr	DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
title_full_unstemmed	DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia
title_sort	dgkδ triggers endoplasmic reticulum release of ift88-containing vesicles destined for the assembly of primary cilia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e5210e7b77f24980b5cd040dcfcd662c
work_keys_str_mv	AT jieding dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT leishao dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT yixingyao dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT xintong dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT huaizeliu dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT shenyue dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT luxie dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia AT stevenycheng dgkdtriggersendoplasmicreticulumreleaseofift88containingvesiclesdestinedfortheassemblyofprimarycilia
_version_	1718395676792979456

DGKδ triggers endoplasmic reticulum release of IFT88-containing vesicles destined for the assembly of primary cilia

Ejemplares similares