From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.

The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles' bites were mainly related to coagulation, while those caused by adults' bites...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniela Miki Hatakeyama, Lídia Jorge Tasima, Nathália da Costa Galizio, Caroline Serino-Silva, Caroline Fabri Bittencourt Rodrigues, Daniel Rodrigues Stuginski, Sávio Stefanini Sant'Anna, Kathleen Fernandes Grego, Alexandre Keiji Tashima, Erika Sayuri Nishiduka, Karen de Morais-Zani, Anita Mitico Tanaka-Azevedo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e521c8b27dbc4911b60e084c340fadb9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e521c8b27dbc4911b60e084c340fadb9
record_format	dspace
spelling	oai:doaj.org-article:e521c8b27dbc4911b60e084c340fadb92021-12-02T20:10:47ZFrom birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.1932-620310.1371/journal.pone.0253050https://doaj.org/article/e521c8b27dbc4911b60e084c340fadb92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253050https://doaj.org/toc/1932-6203The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles' bites were mainly related to coagulation, while those caused by adults' bites had a more prominent local damage. In this work, we analyzed the venoms of B. moojeni at different life stages to better understand the ontogeny shift in this species. Snakes were grouped by age and sex, and venom pools were formed accordingly. Compositional analyses by one-dimensional electrophoresis (1-DE), chromatography, and mass spectrometry revealed that ontogenetic changes might be mostly related to phospholipase A2 (PLA2) and metalloproteases. Regarding the venoms functional aspect, proteolytic, L-amino acid oxidase, PLA2, and coagulant in vitro activities were assayed, but only the first and the last ones showed age-related changes, with the venom of snakes up to 1 year-old displaying lower proteolytic and higher coagulant activities, while those from 2 years-old onward presented the opposite relation. The venoms of 3 years-old snakes were exceptions to the compositional and functional pattern of adults as both venoms presented profiles similar to neonates. Sex-related differences were observed in specific groups and were not age-related. In vivo experiments (median lethal dose and hemorrhagic activity) were statistically similar between neonates and adults, however we verified that the adult venom killed mice faster comparing to the neonates. All venoms were mostly recognized by the antibothropic serum and displayed similar profiles to 1-DE in western blotting. In conclusion, the Brazilian lancehead venom showed ontogenetic shift in its composition and activities. Furthermore, this change occurred in snakes from 1 to 2 years-old, and interestingly the venom pools from 3 years-old snakes had particular characteristics, which highlights the importance of comprehensive studies to better understand venom variability.Daniela Miki HatakeyamaLídia Jorge TasimaNathália da Costa GalizioCaroline Serino-SilvaCaroline Fabri Bittencourt RodriguesDaniel Rodrigues StuginskiSávio Stefanini Sant'AnnaKathleen Fernandes GregoAlexandre Keiji TashimaErika Sayuri NishidukaKaren de Morais-ZaniAnita Mitico Tanaka-AzevedoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253050 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Miki Hatakeyama Lídia Jorge Tasima Nathália da Costa Galizio Caroline Serino-Silva Caroline Fabri Bittencourt Rodrigues Daniel Rodrigues Stuginski Sávio Stefanini Sant'Anna Kathleen Fernandes Grego Alexandre Keiji Tashima Erika Sayuri Nishiduka Karen de Morais-Zani Anita Mitico Tanaka-Azevedo From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
description	The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles' bites were mainly related to coagulation, while those caused by adults' bites had a more prominent local damage. In this work, we analyzed the venoms of B. moojeni at different life stages to better understand the ontogeny shift in this species. Snakes were grouped by age and sex, and venom pools were formed accordingly. Compositional analyses by one-dimensional electrophoresis (1-DE), chromatography, and mass spectrometry revealed that ontogenetic changes might be mostly related to phospholipase A2 (PLA2) and metalloproteases. Regarding the venoms functional aspect, proteolytic, L-amino acid oxidase, PLA2, and coagulant in vitro activities were assayed, but only the first and the last ones showed age-related changes, with the venom of snakes up to 1 year-old displaying lower proteolytic and higher coagulant activities, while those from 2 years-old onward presented the opposite relation. The venoms of 3 years-old snakes were exceptions to the compositional and functional pattern of adults as both venoms presented profiles similar to neonates. Sex-related differences were observed in specific groups and were not age-related. In vivo experiments (median lethal dose and hemorrhagic activity) were statistically similar between neonates and adults, however we verified that the adult venom killed mice faster comparing to the neonates. All venoms were mostly recognized by the antibothropic serum and displayed similar profiles to 1-DE in western blotting. In conclusion, the Brazilian lancehead venom showed ontogenetic shift in its composition and activities. Furthermore, this change occurred in snakes from 1 to 2 years-old, and interestingly the venom pools from 3 years-old snakes had particular characteristics, which highlights the importance of comprehensive studies to better understand venom variability.
format	article
author	Daniela Miki Hatakeyama Lídia Jorge Tasima Nathália da Costa Galizio Caroline Serino-Silva Caroline Fabri Bittencourt Rodrigues Daniel Rodrigues Stuginski Sávio Stefanini Sant'Anna Kathleen Fernandes Grego Alexandre Keiji Tashima Erika Sayuri Nishiduka Karen de Morais-Zani Anita Mitico Tanaka-Azevedo
author_facet	Daniela Miki Hatakeyama Lídia Jorge Tasima Nathália da Costa Galizio Caroline Serino-Silva Caroline Fabri Bittencourt Rodrigues Daniel Rodrigues Stuginski Sávio Stefanini Sant'Anna Kathleen Fernandes Grego Alexandre Keiji Tashima Erika Sayuri Nishiduka Karen de Morais-Zani Anita Mitico Tanaka-Azevedo
author_sort	Daniela Miki Hatakeyama
title	From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
title_short	From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
title_full	From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
title_fullStr	From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
title_full_unstemmed	From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.
title_sort	from birth to adulthood: an analysis of the brazilian lancehead (bothrops moojeni) venom at different life stages.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e521c8b27dbc4911b60e084c340fadb9
work_keys_str_mv	AT danielamikihatakeyama frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT lidiajorgetasima frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT nathaliadacostagalizio frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT carolineserinosilva frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT carolinefabribittencourtrodrigues frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT danielrodriguesstuginski frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT saviostefaninisantanna frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT kathleenfernandesgrego frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT alexandrekeijitashima frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT erikasayurinishiduka frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT karendemoraiszani frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages AT anitamiticotanakaazevedo frombirthtoadulthoodananalysisofthebrazilianlanceheadbothropsmoojenivenomatdifferentlifestages
_version_	1718374977626963968

From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages.

Ejemplares similares