xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.

Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Endrick Guy, Martine Lautier, Matthieu Chabannes, Brice Roux, Emmanuelle Lauber, Matthieu Arlat, Laurent D Noël
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5237b948efc4135954c9bca9955a587
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e5237b948efc4135954c9bca9955a587
record_format	dspace
spelling	oai:doaj.org-article:e5237b948efc4135954c9bca9955a5872021-11-18T09:00:22ZxopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.1932-620310.1371/journal.pone.0073469https://doaj.org/article/e5237b948efc4135954c9bca9955a5872013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23951354/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc.Endrick GuyMartine LautierMatthieu ChabannesBrice RouxEmmanuelle LauberMatthieu ArlatLaurent D NoëlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73469 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Endrick Guy Martine Lautier Matthieu Chabannes Brice Roux Emmanuelle Lauber Matthieu Arlat Laurent D Noël xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
description	Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc.
format	article
author	Endrick Guy Martine Lautier Matthieu Chabannes Brice Roux Emmanuelle Lauber Matthieu Arlat Laurent D Noël
author_facet	Endrick Guy Martine Lautier Matthieu Chabannes Brice Roux Emmanuelle Lauber Matthieu Arlat Laurent D Noël
author_sort	Endrick Guy
title	xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
title_short	xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
title_full	xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
title_fullStr	xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
title_full_unstemmed	xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.
title_sort	xopac-triggered immunity against xanthomonas depends on arabidopsis receptor-like cytoplasmic kinase genes pbl2 and ripk.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e5237b948efc4135954c9bca9955a587
work_keys_str_mv	AT endrickguy xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT martinelautier xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT matthieuchabannes xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT briceroux xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT emmanuellelauber xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT matthieuarlat xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk AT laurentdnoel xopactriggeredimmunityagainstxanthomonasdependsonarabidopsisreceptorlikecytoplasmickinasegenespbl2andripk
_version_	1718421056370245632

xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK.

Ejemplares similares