Activated neutrophil fluorescent imaging technique for human lungs

Abstract Neutrophil activation is an integral process to acute inflammation and is associated with adverse clinical sequelae. Identification of neutrophil activation in real time in the lungs of patients may permit biological stratification of patients in otherwise heterogenous cohorts typically def...

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Autores principales:	Thomas H. Craven, Tashfeen Walton, Ahsan R. Akram, Emma Scholefield, Neil McDonald, Adam D.L. Marshall, Duncan C. Humphries, Bethany Mills, Thane A. Campbell, Annya Bruce, Joanne Mair, James W. Dear, David E. Newby, Adam T. Hill, Timothy S. Walsh, Chris Haslett, Kevin Dhaliwal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e52c1d397194451fb7aab2ac97bdda7d
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spelling	oai:doaj.org-article:e52c1d397194451fb7aab2ac97bdda7d2021-12-02T14:12:42ZActivated neutrophil fluorescent imaging technique for human lungs10.1038/s41598-020-80083-w2045-2322https://doaj.org/article/e52c1d397194451fb7aab2ac97bdda7d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80083-whttps://doaj.org/toc/2045-2322Abstract Neutrophil activation is an integral process to acute inflammation and is associated with adverse clinical sequelae. Identification of neutrophil activation in real time in the lungs of patients may permit biological stratification of patients in otherwise heterogenous cohorts typically defined by clinical criteria. No methods for identifying neutrophil activation in real time in the lungs of patients currently exist. We developed a bespoke molecular imaging probe targeting three characteristic signatures of neutrophil activation: pinocytosis, phagosomal alkalinisation, and human neutrophil elastase (HNE) activity. The probe functioned as designed in vitro and ex vivo. We evaluated optical endomicroscopy imaging of neutrophil activity using the probe in real-time at the bedside of healthy volunteers, patients with bronchiectasis, and critically unwell mechanically ventilated patients. We detected a range of imaging responses in vivo reflecting heterogeneity of condition and severity. We corroborated optical signal was due to probe function and neutrophil activation.Thomas H. CravenTashfeen WaltonAhsan R. AkramEmma ScholefieldNeil McDonaldAdam D.L. MarshallDuncan C. HumphriesBethany MillsThane A. CampbellAnnya BruceJoanne MairJames W. DearDavid E. NewbyAdam T. HillTimothy S. WalshChris HaslettKevin DhaliwalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas H. Craven Tashfeen Walton Ahsan R. Akram Emma Scholefield Neil McDonald Adam D.L. Marshall Duncan C. Humphries Bethany Mills Thane A. Campbell Annya Bruce Joanne Mair James W. Dear David E. Newby Adam T. Hill Timothy S. Walsh Chris Haslett Kevin Dhaliwal Activated neutrophil fluorescent imaging technique for human lungs
description	Abstract Neutrophil activation is an integral process to acute inflammation and is associated with adverse clinical sequelae. Identification of neutrophil activation in real time in the lungs of patients may permit biological stratification of patients in otherwise heterogenous cohorts typically defined by clinical criteria. No methods for identifying neutrophil activation in real time in the lungs of patients currently exist. We developed a bespoke molecular imaging probe targeting three characteristic signatures of neutrophil activation: pinocytosis, phagosomal alkalinisation, and human neutrophil elastase (HNE) activity. The probe functioned as designed in vitro and ex vivo. We evaluated optical endomicroscopy imaging of neutrophil activity using the probe in real-time at the bedside of healthy volunteers, patients with bronchiectasis, and critically unwell mechanically ventilated patients. We detected a range of imaging responses in vivo reflecting heterogeneity of condition and severity. We corroborated optical signal was due to probe function and neutrophil activation.
format	article
author	Thomas H. Craven Tashfeen Walton Ahsan R. Akram Emma Scholefield Neil McDonald Adam D.L. Marshall Duncan C. Humphries Bethany Mills Thane A. Campbell Annya Bruce Joanne Mair James W. Dear David E. Newby Adam T. Hill Timothy S. Walsh Chris Haslett Kevin Dhaliwal
author_facet	Thomas H. Craven Tashfeen Walton Ahsan R. Akram Emma Scholefield Neil McDonald Adam D.L. Marshall Duncan C. Humphries Bethany Mills Thane A. Campbell Annya Bruce Joanne Mair James W. Dear David E. Newby Adam T. Hill Timothy S. Walsh Chris Haslett Kevin Dhaliwal
author_sort	Thomas H. Craven
title	Activated neutrophil fluorescent imaging technique for human lungs
title_short	Activated neutrophil fluorescent imaging technique for human lungs
title_full	Activated neutrophil fluorescent imaging technique for human lungs
title_fullStr	Activated neutrophil fluorescent imaging technique for human lungs
title_full_unstemmed	Activated neutrophil fluorescent imaging technique for human lungs
title_sort	activated neutrophil fluorescent imaging technique for human lungs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e52c1d397194451fb7aab2ac97bdda7d
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Activated neutrophil fluorescent imaging technique for human lungs

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