Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.

<h4>Objective</h4>To investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls.<h4>Design</h4>The microbiota in biopsies was investigated utilizing a novel molecular method...

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Autores principales:	Tore Midtvedt, Eugene Zabarovsky, Elisabeth Norin, Johan Bark, Rinat Gizatullin, Vladimir Kashuba, Olle Ljungqvist, Veronika Zabarovska, Roland Möllby, Ingemar Ernberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5357963fea94290a24b33fcdf2f4a95
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spelling	oai:doaj.org-article:e5357963fea94290a24b33fcdf2f4a952021-11-18T07:40:51ZIncrease of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.1932-620310.1371/journal.pone.0066074https://doaj.org/article/e5357963fea94290a24b33fcdf2f4a952013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840402/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls.<h4>Design</h4>The microbiota in biopsies was investigated utilizing a novel molecular method and classical cultivation technology. Faecal samples were investigated by classical technology and four functional methods, reflecting alterations in short chain fatty acids pattern, conversion of cholesterol and bilirubin and inactivation of trypsin.<h4>Results</h4>By molecular methods we found more than 92% similarity in the microbiota on the biopsies from the two groups. However, 4.6% of microbes found in controls were lacking in CD patients. Furthermore, NotI representation libraries demonstrate two different clusters representing CD patients and controls, respectively. Utilizing conventional technology, Bacteroides (alt. Parabacteroides) was less frequently detected in the biopsies from CD patients than from controls. A similar reduction in the number of Bacteroides was found in faecal samples. Bacteroides is the only group of bacteria known to be able to inactivate pancreatic trypsin. Faecal tryptic activity was high in CD patients, and inversely correlated to the levels of Bacteroides.<h4>Conclusions</h4>CD patients have compositional and functional alterations in their intestinal microbiota, in line with the global description hypothesis rather than the candidate microorganism theory. The most striking functional difference was high amount of faecal tryptic activity in CD patients, inversely correlated to the levels of Bacteroides in faeces.Tore MidtvedtEugene ZabarovskyElisabeth NorinJohan BarkRinat GizatullinVladimir KashubaOlle LjungqvistVeronika ZabarovskaRoland MöllbyIngemar ErnbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66074 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tore Midtvedt Eugene Zabarovsky Elisabeth Norin Johan Bark Rinat Gizatullin Vladimir Kashuba Olle Ljungqvist Veronika Zabarovska Roland Möllby Ingemar Ernberg Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
description	<h4>Objective</h4>To investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls.<h4>Design</h4>The microbiota in biopsies was investigated utilizing a novel molecular method and classical cultivation technology. Faecal samples were investigated by classical technology and four functional methods, reflecting alterations in short chain fatty acids pattern, conversion of cholesterol and bilirubin and inactivation of trypsin.<h4>Results</h4>By molecular methods we found more than 92% similarity in the microbiota on the biopsies from the two groups. However, 4.6% of microbes found in controls were lacking in CD patients. Furthermore, NotI representation libraries demonstrate two different clusters representing CD patients and controls, respectively. Utilizing conventional technology, Bacteroides (alt. Parabacteroides) was less frequently detected in the biopsies from CD patients than from controls. A similar reduction in the number of Bacteroides was found in faecal samples. Bacteroides is the only group of bacteria known to be able to inactivate pancreatic trypsin. Faecal tryptic activity was high in CD patients, and inversely correlated to the levels of Bacteroides.<h4>Conclusions</h4>CD patients have compositional and functional alterations in their intestinal microbiota, in line with the global description hypothesis rather than the candidate microorganism theory. The most striking functional difference was high amount of faecal tryptic activity in CD patients, inversely correlated to the levels of Bacteroides in faeces.
format	article
author	Tore Midtvedt Eugene Zabarovsky Elisabeth Norin Johan Bark Rinat Gizatullin Vladimir Kashuba Olle Ljungqvist Veronika Zabarovska Roland Möllby Ingemar Ernberg
author_facet	Tore Midtvedt Eugene Zabarovsky Elisabeth Norin Johan Bark Rinat Gizatullin Vladimir Kashuba Olle Ljungqvist Veronika Zabarovska Roland Möllby Ingemar Ernberg
author_sort	Tore Midtvedt
title	Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
title_short	Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
title_full	Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
title_fullStr	Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
title_full_unstemmed	Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
title_sort	increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of crohn's disease with a multidisciplinary platform.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e5357963fea94290a24b33fcdf2f4a95
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Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.

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