EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?

Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by infection with the larvae of Echinococcus granulosus sensu lato (s.l.) cluster. It is urgent to identify novel drug targets and develop new drug candidates against CE. Glucose transporter 1 (GLUT1) is mainly responsible for the tra...

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Autores principales:	Kuerbannisha Amahong, Mingzhi Yan, Jintian Li, Ning Yang, Hui Liu, Xiaojuan Bi, Dominique A. Vuitton, Renyong Lin, Guodong Lü
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Echinococcus granulosus sensu stricto glucose transporter 1 WZB117 cystic echinococcosis glucose uptake Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e535f1a31217449589f3fefa21410803
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spelling	oai:doaj.org-article:e535f1a31217449589f3fefa214108032021-11-11T10:13:11ZEgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?2235-298810.3389/fcimb.2021.747739https://doaj.org/article/e535f1a31217449589f3fefa214108032021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.747739/fullhttps://doaj.org/toc/2235-2988Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by infection with the larvae of Echinococcus granulosus sensu lato (s.l.) cluster. It is urgent to identify novel drug targets and develop new drug candidates against CE. Glucose transporter 1 (GLUT1) is mainly responsible for the transmembrane transport of glucose to maintain its constant cellular availability and is a recent research hotspot as a drug target in various diseases. However, the role of GLUT1 in E. granulosus s.l. (EgGLUT1) was unknown. In this study, we cloned a conserved GLUT1 homology gene (named EgGLUT1-ss) from E. granulosus sensu stricto (s.s.) and found EgGLUT1-ss was crucial for glucose uptake and viability by the protoscoleces of E. granulosus s.s. WZB117, a GLUT1 inhibitor, inhibited glucose uptake by E. granulosus s.s. and the viability of the metacestode in vitro. In addition, WZB117 showed significant therapeutic activity in E. granulosus s.s.-infected mice: a 10 mg/kg dose of WZB117 significantly reduced the number and weight of parasite cysts (P < 0.05) as efficiently as the reference drug, albendazole. Our results demonstrate that EgGLUT1-ss is crucial for glucose uptake by the protoscoleces of E. granulosus s.s., and its inhibitor WZB117 has a therapeutic effect on CE.Kuerbannisha AmahongKuerbannisha AmahongMingzhi YanMingzhi YanJintian LiJintian LiNing YangHui LiuXiaojuan BiDominique A. VuittonRenyong LinRenyong LinRenyong LinGuodong LüGuodong LüGuodong LüFrontiers Media S.A.articleEchinococcus granulosus sensu strictoglucose transporter 1WZB117cystic echinococcosisglucose uptakeMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Echinococcus granulosus sensu stricto glucose transporter 1 WZB117 cystic echinococcosis glucose uptake Microbiology QR1-502
spellingShingle	Echinococcus granulosus sensu stricto glucose transporter 1 WZB117 cystic echinococcosis glucose uptake Microbiology QR1-502 Kuerbannisha Amahong Kuerbannisha Amahong Mingzhi Yan Mingzhi Yan Jintian Li Jintian Li Ning Yang Hui Liu Xiaojuan Bi Dominique A. Vuitton Renyong Lin Renyong Lin Renyong Lin Guodong Lü Guodong Lü Guodong Lü EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
description	Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by infection with the larvae of Echinococcus granulosus sensu lato (s.l.) cluster. It is urgent to identify novel drug targets and develop new drug candidates against CE. Glucose transporter 1 (GLUT1) is mainly responsible for the transmembrane transport of glucose to maintain its constant cellular availability and is a recent research hotspot as a drug target in various diseases. However, the role of GLUT1 in E. granulosus s.l. (EgGLUT1) was unknown. In this study, we cloned a conserved GLUT1 homology gene (named EgGLUT1-ss) from E. granulosus sensu stricto (s.s.) and found EgGLUT1-ss was crucial for glucose uptake and viability by the protoscoleces of E. granulosus s.s. WZB117, a GLUT1 inhibitor, inhibited glucose uptake by E. granulosus s.s. and the viability of the metacestode in vitro. In addition, WZB117 showed significant therapeutic activity in E. granulosus s.s.-infected mice: a 10 mg/kg dose of WZB117 significantly reduced the number and weight of parasite cysts (P < 0.05) as efficiently as the reference drug, albendazole. Our results demonstrate that EgGLUT1-ss is crucial for glucose uptake by the protoscoleces of E. granulosus s.s., and its inhibitor WZB117 has a therapeutic effect on CE.
format	article
author	Kuerbannisha Amahong Kuerbannisha Amahong Mingzhi Yan Mingzhi Yan Jintian Li Jintian Li Ning Yang Hui Liu Xiaojuan Bi Dominique A. Vuitton Renyong Lin Renyong Lin Renyong Lin Guodong Lü Guodong Lü Guodong Lü
author_facet	Kuerbannisha Amahong Kuerbannisha Amahong Mingzhi Yan Mingzhi Yan Jintian Li Jintian Li Ning Yang Hui Liu Xiaojuan Bi Dominique A. Vuitton Renyong Lin Renyong Lin Renyong Lin Guodong Lü Guodong Lü Guodong Lü
author_sort	Kuerbannisha Amahong
title	EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
title_short	EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
title_full	EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
title_fullStr	EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
title_full_unstemmed	EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?
title_sort	egglut1 is crucial for the viability of echinococcus granulosus sensu stricto metacestode: a new therapeutic target?
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e535f1a31217449589f3fefa21410803
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EgGLUT1 Is Crucial for the Viability of Echinococcus granulosus sensu stricto Metacestode: A New Therapeutic Target?

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