Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines

Masahiko Ayaki1, Atsuo Iwasawa21Department of Ophthalmology, Saitama National Hospital, Wako, 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, JapanPurpose: This study evaluated the cytotoxicity of five prostaglandin analog ophthalmic &shy;solutions on four o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Masahiko Ayaki, Atsuo Iwasawa
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/e53a3af8864649a19a5eff63e4b06761
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e53a3af8864649a19a5eff63e4b06761
record_format	dspace
spelling	oai:doaj.org-article:e53a3af8864649a19a5eff63e4b067612021-12-02T05:31:11ZCytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines1177-54671177-5483https://doaj.org/article/e53a3af8864649a19a5eff63e4b067612010-08-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-prostaglandin-analog-eye-drops-preserved-with-benzalko-a5061https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Masahiko Ayaki1, Atsuo Iwasawa21Department of Ophthalmology, Saitama National Hospital, Wako, 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, JapanPurpose: This study evaluated the cytotoxicity of five prostaglandin analog ophthalmic &shy;solutions on four ocular surface cell lines, ie, Chang (human conjunctiva), SIRC (rabbit cornea), RC-1 (rabbit cornea), and BCE C/D-1b (bovine cornea).Methods: Cell viability was measured by neutral red and MTT assays in cells treated for 10, 30, or 60 minutes with various doses of prostaglandins (undiluted, and 2- and 10-fold dilutions). The number of cell lines with viability &ge;50% in the presence of selected dilution of the drug (CVS50) was used for comparison. In addition, 24 cell viability comparisons (four cell lines, two assays, and three exposure times) were made between latanoprost (Xalatan&reg;) and each other solution at each dose. A comparison between the newly introduced tafluprost (Tapros&reg;) with 0.01% benzalkonium chloride was also made.Results: The order of cell viability determined by CVS50 was Travatan Z&reg; (travoprost with the SofZia system)&gt; Tapros &ge; Travatan&reg; (travoprost) = Xalatan &gt; Rescula&reg; (unoproston). This was consistent with the results of direct comparisons between Xalatan and the other drugs. There was no clear difference in cell viability between Tapros and benzalkonium chloride.Conclusions: Use of two assays, multiple cell lines, and various dilutions and exposure times provided a unique evaluation of cytotoxicity among ophthalmic solutions. CVS50 was useful for comparison of the cell viability of the solutions.Keywords: glaucoma, cornea, cell viability score Masahiko AyakiAtsuo IwasawaDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 919-924 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Masahiko Ayaki Atsuo Iwasawa Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
description	Masahiko Ayaki1, Atsuo Iwasawa21Department of Ophthalmology, Saitama National Hospital, Wako, 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, JapanPurpose: This study evaluated the cytotoxicity of five prostaglandin analog ophthalmic &shy;solutions on four ocular surface cell lines, ie, Chang (human conjunctiva), SIRC (rabbit cornea), RC-1 (rabbit cornea), and BCE C/D-1b (bovine cornea).Methods: Cell viability was measured by neutral red and MTT assays in cells treated for 10, 30, or 60 minutes with various doses of prostaglandins (undiluted, and 2- and 10-fold dilutions). The number of cell lines with viability &ge;50% in the presence of selected dilution of the drug (CVS50) was used for comparison. In addition, 24 cell viability comparisons (four cell lines, two assays, and three exposure times) were made between latanoprost (Xalatan&reg;) and each other solution at each dose. A comparison between the newly introduced tafluprost (Tapros&reg;) with 0.01% benzalkonium chloride was also made.Results: The order of cell viability determined by CVS50 was Travatan Z&reg; (travoprost with the SofZia system)&gt; Tapros &ge; Travatan&reg; (travoprost) = Xalatan &gt; Rescula&reg; (unoproston). This was consistent with the results of direct comparisons between Xalatan and the other drugs. There was no clear difference in cell viability between Tapros and benzalkonium chloride.Conclusions: Use of two assays, multiple cell lines, and various dilutions and exposure times provided a unique evaluation of cytotoxicity among ophthalmic solutions. CVS50 was useful for comparison of the cell viability of the solutions.Keywords: glaucoma, cornea, cell viability score
format	article
author	Masahiko Ayaki Atsuo Iwasawa
author_facet	Masahiko Ayaki Atsuo Iwasawa
author_sort	Masahiko Ayaki
title	Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
title_short	Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
title_full	Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
title_fullStr	Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
title_full_unstemmed	Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
title_sort	cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/e53a3af8864649a19a5eff63e4b06761
work_keys_str_mv	AT masahikoayaki cytotoxicityofprostaglandinanalogeyedropspreservedwithbenzalkoniumchlorideinmultiplecorneoconjunctivalcelllines AT atsuoiwasawa cytotoxicityofprostaglandinanalogeyedropspreservedwithbenzalkoniumchlorideinmultiplecorneoconjunctivalcelllines
_version_	1718400383558090752

Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines

Ejemplares similares