Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants

Melon fly (<i>Bactrocera cucurbitae</i>) is the most common pest of cucurbits, and it directly causes damage to cucurbit fruits in the early developmental stage. The infection of fruit tissues induces oxidative damage through increased generation of cellular reactive oxygen species. The...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Madhusudana Somegowda, S. Raghavendra, Shankarappa Sridhara, Achur. N. Rajeshwara, Siddanakoppalu. N. Pramod, S. Shivashankar, Feng Lin, Tarek K Zin El-Abedin, Shabir Hussain Wani, Hosam O. Elansary
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	melon fly ROS antioxidant defensive enzymes phenols and flavonoids superoxide dismutase Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/e53adee615a74ee39f71cb58b19af56b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e53adee615a74ee39f71cb58b19af56b
record_format	dspace
spelling	oai:doaj.org-article:e53adee615a74ee39f71cb58b19af56b2021-11-11T18:22:40ZDefensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants10.3390/molecules262163451420-3049https://doaj.org/article/e53adee615a74ee39f71cb58b19af56b2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6345https://doaj.org/toc/1420-3049Melon fly (<i>Bactrocera cucurbitae</i>) is the most common pest of cucurbits, and it directly causes damage to cucurbit fruits in the early developmental stage. The infection of fruit tissues induces oxidative damage through increased generation of cellular reactive oxygen species. The effects of melon fly infestation on the production of defensive enzymes and antioxidant capabilities in five cucurbit species, namely, bottle gourd, chayote, cucumber, snake gourd, and bitter gourd, were investigated in this study. The total phenolic and flavonoid content was considerably higher in melon fly infestation tissues compared to healthy and apparently healthy tissues. The chayote and bottle gourd tissues expressed almost 1.5- to 2-fold higher phenolic and flavonoid contents compared to the tissues of bitter gourd, snake gourd, and cucumber upon infestation. Defensive enzymes, such as peroxidase (POD), superoxide dismutase (SOD), polyphenol oxidase (PPO), and catalase (CAT), were high in healthy and infected tissues of chayote and bottle gourd compared to bitter gourd, snake gourd, and cucumber. The activity of POD (60–80%), SOD (30–35%), PPO (70–75%), and CAT (40–50%) were high in infected chayote and bottle gourd tissue, representing resistance against infestation, while bitter gourd, snake gourd, and cucumber exhibited comparatively lower activity suggesting susceptibility to melon fly infection. The antioxidant properties were also high in the resistant cucurbits compared to the susceptible cucurbits. The current research has enlightened the importance of redox-regulatory pathways involving ROS neutralization through infection-induced antioxidative enzymes in host cucurbit resistance. The melon fly infestation depicts the possible induction of pathways that upregulate the production of defensive enzymes and antioxidants as a defensive strategy against melon fly infestation in resistant cucurbits.Madhusudana SomegowdaS. RaghavendraShankarappa SridharaAchur. N. RajeshwaraSiddanakoppalu. N. PramodS. ShivashankarFeng LinTarek K Zin El-AbedinShabir Hussain WaniHosam O. ElansaryMDPI AGarticlemelon flyROSantioxidantdefensive enzymesphenols and flavonoidssuperoxide dismutaseOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6345, p 6345 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melon fly ROS antioxidant defensive enzymes phenols and flavonoids superoxide dismutase Organic chemistry QD241-441
spellingShingle	melon fly ROS antioxidant defensive enzymes phenols and flavonoids superoxide dismutase Organic chemistry QD241-441 Madhusudana Somegowda S. Raghavendra Shankarappa Sridhara Achur. N. Rajeshwara Siddanakoppalu. N. Pramod S. Shivashankar Feng Lin Tarek K Zin El-Abedin Shabir Hussain Wani Hosam O. Elansary Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
description	Melon fly (<i>Bactrocera cucurbitae</i>) is the most common pest of cucurbits, and it directly causes damage to cucurbit fruits in the early developmental stage. The infection of fruit tissues induces oxidative damage through increased generation of cellular reactive oxygen species. The effects of melon fly infestation on the production of defensive enzymes and antioxidant capabilities in five cucurbit species, namely, bottle gourd, chayote, cucumber, snake gourd, and bitter gourd, were investigated in this study. The total phenolic and flavonoid content was considerably higher in melon fly infestation tissues compared to healthy and apparently healthy tissues. The chayote and bottle gourd tissues expressed almost 1.5- to 2-fold higher phenolic and flavonoid contents compared to the tissues of bitter gourd, snake gourd, and cucumber upon infestation. Defensive enzymes, such as peroxidase (POD), superoxide dismutase (SOD), polyphenol oxidase (PPO), and catalase (CAT), were high in healthy and infected tissues of chayote and bottle gourd compared to bitter gourd, snake gourd, and cucumber. The activity of POD (60–80%), SOD (30–35%), PPO (70–75%), and CAT (40–50%) were high in infected chayote and bottle gourd tissue, representing resistance against infestation, while bitter gourd, snake gourd, and cucumber exhibited comparatively lower activity suggesting susceptibility to melon fly infection. The antioxidant properties were also high in the resistant cucurbits compared to the susceptible cucurbits. The current research has enlightened the importance of redox-regulatory pathways involving ROS neutralization through infection-induced antioxidative enzymes in host cucurbit resistance. The melon fly infestation depicts the possible induction of pathways that upregulate the production of defensive enzymes and antioxidants as a defensive strategy against melon fly infestation in resistant cucurbits.
format	article
author	Madhusudana Somegowda S. Raghavendra Shankarappa Sridhara Achur. N. Rajeshwara Siddanakoppalu. N. Pramod S. Shivashankar Feng Lin Tarek K Zin El-Abedin Shabir Hussain Wani Hosam O. Elansary
author_facet	Madhusudana Somegowda S. Raghavendra Shankarappa Sridhara Achur. N. Rajeshwara Siddanakoppalu. N. Pramod S. Shivashankar Feng Lin Tarek K Zin El-Abedin Shabir Hussain Wani Hosam O. Elansary
author_sort	Madhusudana Somegowda
title	Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
title_short	Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
title_full	Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
title_fullStr	Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
title_full_unstemmed	Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants
title_sort	defensive mechanisms in cucurbits against melon fly (<i>bactrocera cucurbitae</i>) infestation through excessive production of defensive enzymes and antioxidants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e53adee615a74ee39f71cb58b19af56b
work_keys_str_mv	AT madhusudanasomegowda defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT sraghavendra defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT shankarappasridhara defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT achurnrajeshwara defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT siddanakoppalunpramod defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT sshivashankar defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT fenglin defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT tarekkzinelabedin defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT shabirhussainwani defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants AT hosamoelansary defensivemechanismsincucurbitsagainstmelonflyibactroceracucurbitaeiinfestationthroughexcessiveproductionofdefensiveenzymesandantioxidants
_version_	1718431856209166336

Defensive Mechanisms in Cucurbits against Melon Fly (<i>Bactrocera cucurbitae</i>) Infestation through Excessive Production of Defensive Enzymes and Antioxidants

Ejemplares similares