Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

DNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II to shape mitotic chromosome organization. In vitro chromatin r...

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Autores principales:	Pavan Choppakatla, Bastiaan Dekker, Erin E Cutts, Alessandro Vannini, Job Dekker, Hironori Funabiki
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	chromosome compaction mitosis linker histone nucleosome Hi-C chromatin Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e53d35ae1e1748bb8cdf3a62b3e3e7a3
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spelling	oai:doaj.org-article:e53d35ae1e1748bb8cdf3a62b3e3e7a32021-11-26T07:06:35ZLinker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization10.7554/eLife.689182050-084Xe68918https://doaj.org/article/e53d35ae1e1748bb8cdf3a62b3e3e7a32021-08-01T00:00:00Zhttps://elifesciences.org/articles/68918https://doaj.org/toc/2050-084XDNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II to shape mitotic chromosome organization. In vitro chromatin reconstitution experiments demonstrate that H1.8 inhibits binding of condensins and topo II to nucleosome arrays. Accordingly, H1.8 depletion in Xenopus egg extracts increased condensins and topo II levels on mitotic chromatin. Chromosome morphology and Hi-C analyses suggest that H1.8 depletion makes chromosomes thinner and longer through shortening the average loop size and reducing the DNA amount in each layer of mitotic loops. Furthermore, excess loading of condensins and topo II to chromosomes by H1.8 depletion causes hyper-chromosome individualization and dispersion. We propose that condensins and topo II are essential for chromosome individualization, but their functions are tuned by the linker histone to keep chromosomes together until anaphase.Pavan ChoppakatlaBastiaan DekkerErin E CuttsAlessandro VanniniJob DekkerHironori FunabikieLife Sciences Publications Ltdarticlechromosome compactionmitosislinker histonenucleosomeHi-CchromatinMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromosome compaction mitosis linker histone nucleosome Hi-C chromatin Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	chromosome compaction mitosis linker histone nucleosome Hi-C chromatin Medicine R Science Q Biology (General) QH301-705.5 Pavan Choppakatla Bastiaan Dekker Erin E Cutts Alessandro Vannini Job Dekker Hironori Funabiki Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
description	DNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II to shape mitotic chromosome organization. In vitro chromatin reconstitution experiments demonstrate that H1.8 inhibits binding of condensins and topo II to nucleosome arrays. Accordingly, H1.8 depletion in Xenopus egg extracts increased condensins and topo II levels on mitotic chromatin. Chromosome morphology and Hi-C analyses suggest that H1.8 depletion makes chromosomes thinner and longer through shortening the average loop size and reducing the DNA amount in each layer of mitotic loops. Furthermore, excess loading of condensins and topo II to chromosomes by H1.8 depletion causes hyper-chromosome individualization and dispersion. We propose that condensins and topo II are essential for chromosome individualization, but their functions are tuned by the linker histone to keep chromosomes together until anaphase.
format	article
author	Pavan Choppakatla Bastiaan Dekker Erin E Cutts Alessandro Vannini Job Dekker Hironori Funabiki
author_facet	Pavan Choppakatla Bastiaan Dekker Erin E Cutts Alessandro Vannini Job Dekker Hironori Funabiki
author_sort	Pavan Choppakatla
title	Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
title_short	Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
title_full	Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
title_fullStr	Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
title_full_unstemmed	Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
title_sort	linker histone h1.8 inhibits chromatin binding of condensins and dna topoisomerase ii to tune chromosome length and individualization
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/e53d35ae1e1748bb8cdf3a62b3e3e7a3
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_version_	1718409750701408256

Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

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