Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.

Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell cul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S Lucas Black, Angela Dawson, F Bruce Ward, Rosalind J Allen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5400b8f184947b9a8b9abca7524c5d0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e5400b8f184947b9a8b9abca7524c5d0
record_format	dspace
spelling	oai:doaj.org-article:e5400b8f184947b9a8b9abca7524c5d02021-11-18T08:55:45ZGenes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.1932-620310.1371/journal.pone.0073995https://doaj.org/article/e5400b8f184947b9a8b9abca7524c5d02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24040140/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell culture method, to investigate the effects of hydrostatic pressure on microbial physiology in vivo. The Keio collection of single-gene deletion mutants in Escherichia coli K-12 was screened for growth at a range of pressures from 0.1 MPa to 60 MPa. This led to the identification of 6 genes, rodZ, holC, priA, dnaT, dedD and tatC, whose products were required for growth at 30 MPa and a further 3 genes, tolB, rffT and iscS, whose products were required for growth at 40 MPa. Our results support the view that the effects of pressure on cell physiology are pleiotropic, with DNA replication, cell division, the cytoskeleton and cell envelope physiology all being potential failure points for cell physiology during growth at elevated pressure.S Lucas BlackAngela DawsonF Bruce WardRosalind J AllenRosalind J AllenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73995 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S Lucas Black Angela Dawson F Bruce Ward Rosalind J Allen Rosalind J Allen Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
description	Despite the fact that much of the global microbial biosphere is believed to exist in high pressure environments, the effects of hydrostatic pressure on microbial physiology remain poorly understood. We use a genome-wide screening approach, combined with a novel high-throughput high-pressure cell culture method, to investigate the effects of hydrostatic pressure on microbial physiology in vivo. The Keio collection of single-gene deletion mutants in Escherichia coli K-12 was screened for growth at a range of pressures from 0.1 MPa to 60 MPa. This led to the identification of 6 genes, rodZ, holC, priA, dnaT, dedD and tatC, whose products were required for growth at 30 MPa and a further 3 genes, tolB, rffT and iscS, whose products were required for growth at 40 MPa. Our results support the view that the effects of pressure on cell physiology are pleiotropic, with DNA replication, cell division, the cytoskeleton and cell envelope physiology all being potential failure points for cell physiology during growth at elevated pressure.
format	article
author	S Lucas Black Angela Dawson F Bruce Ward Rosalind J Allen Rosalind J Allen
author_facet	S Lucas Black Angela Dawson F Bruce Ward Rosalind J Allen Rosalind J Allen
author_sort	S Lucas Black
title	Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
title_short	Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
title_full	Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
title_fullStr	Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
title_full_unstemmed	Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.
title_sort	genes required for growth at high hydrostatic pressure in escherichia coli k-12 identified by genome-wide screening.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e5400b8f184947b9a8b9abca7524c5d0
work_keys_str_mv	AT slucasblack genesrequiredforgrowthathighhydrostaticpressureinescherichiacolik12identifiedbygenomewidescreening AT angeladawson genesrequiredforgrowthathighhydrostaticpressureinescherichiacolik12identifiedbygenomewidescreening AT fbruceward genesrequiredforgrowthathighhydrostaticpressureinescherichiacolik12identifiedbygenomewidescreening AT rosalindjallen genesrequiredforgrowthathighhydrostaticpressureinescherichiacolik12identifiedbygenomewidescreening AT rosalindjallen genesrequiredforgrowthathighhydrostaticpressureinescherichiacolik12identifiedbygenomewidescreening
_version_	1718421173983772672

Genes required for growth at high hydrostatic pressure in Escherichia coli K-12 identified by genome-wide screening.

Ejemplares similares