Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice

Abstract The Kif26a protein-coding gene has been identified as a negative regulator of the GDNF-Ret signaling pathway in enteric neurons. The aim of this study was to investigate the influence of genetic background on the phenotype of Kif26a-deficient (KO, −/−) mice. KO mice with both C57BL/6 and BA...

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Autores principales:	Yukiko Ohara, Lisa Fujimura, Akemi Sakamoto, Youichi Teratake, Shuichi Hiraoka, Haruhiko Koseki, Takeshi Saito, Keita Terui, Tetsuya Mitsunaga, Mitsuyuki Nakata, Hideo Yoshida, Masahiko Hatano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e54ea010d9e448fdb8e7a219345c7727
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spelling	oai:doaj.org-article:e54ea010d9e448fdb8e7a219345c77272021-12-02T14:06:49ZGenetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice10.1038/s41598-021-82785-12045-2322https://doaj.org/article/e54ea010d9e448fdb8e7a219345c77272021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82785-1https://doaj.org/toc/2045-2322Abstract The Kif26a protein-coding gene has been identified as a negative regulator of the GDNF-Ret signaling pathway in enteric neurons. The aim of this study was to investigate the influence of genetic background on the phenotype of Kif26a-deficient (KO, −/−) mice. KO mice with both C57BL/6 and BALB/c genetic backgrounds were established. Survival rates and megacolon development were compared between these two strains of KO mice. Functional bowel assessments and enteric neuron histopathology were performed in the deficient mice. KO mice with the BALB/c genetic background survived more than 400 days without evidence of megacolon, while all C57BL/6 KO mice developed megacolon and died within 30 days. Local enteric neuron hyperplasia in the colon and functional bowel abnormalities were observed in BALB/c KO mice. These results indicated that megacolon and enteric neuron hyperplasia in KO mice are influenced by the genetic background. BALB/c KO mice may represent a viable model for functional gastrointestinal diseases such as chronic constipation, facilitating studies on the underlying mechanisms and providing a foundation for the development of treatments.Yukiko OharaLisa FujimuraAkemi SakamotoYouichi TeratakeShuichi HiraokaHaruhiko KosekiTakeshi SaitoKeita TeruiTetsuya MitsunagaMitsuyuki NakataHideo YoshidaMasahiko HatanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yukiko Ohara Lisa Fujimura Akemi Sakamoto Youichi Teratake Shuichi Hiraoka Haruhiko Koseki Takeshi Saito Keita Terui Tetsuya Mitsunaga Mitsuyuki Nakata Hideo Yoshida Masahiko Hatano Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
description	Abstract The Kif26a protein-coding gene has been identified as a negative regulator of the GDNF-Ret signaling pathway in enteric neurons. The aim of this study was to investigate the influence of genetic background on the phenotype of Kif26a-deficient (KO, −/−) mice. KO mice with both C57BL/6 and BALB/c genetic backgrounds were established. Survival rates and megacolon development were compared between these two strains of KO mice. Functional bowel assessments and enteric neuron histopathology were performed in the deficient mice. KO mice with the BALB/c genetic background survived more than 400 days without evidence of megacolon, while all C57BL/6 KO mice developed megacolon and died within 30 days. Local enteric neuron hyperplasia in the colon and functional bowel abnormalities were observed in BALB/c KO mice. These results indicated that megacolon and enteric neuron hyperplasia in KO mice are influenced by the genetic background. BALB/c KO mice may represent a viable model for functional gastrointestinal diseases such as chronic constipation, facilitating studies on the underlying mechanisms and providing a foundation for the development of treatments.
format	article
author	Yukiko Ohara Lisa Fujimura Akemi Sakamoto Youichi Teratake Shuichi Hiraoka Haruhiko Koseki Takeshi Saito Keita Terui Tetsuya Mitsunaga Mitsuyuki Nakata Hideo Yoshida Masahiko Hatano
author_facet	Yukiko Ohara Lisa Fujimura Akemi Sakamoto Youichi Teratake Shuichi Hiraoka Haruhiko Koseki Takeshi Saito Keita Terui Tetsuya Mitsunaga Mitsuyuki Nakata Hideo Yoshida Masahiko Hatano
author_sort	Yukiko Ohara
title	Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
title_short	Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
title_full	Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
title_fullStr	Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
title_full_unstemmed	Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice
title_sort	genetic background-dependent abnormalities of the enteric nervous system and intestinal function in kif26a-deficient mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e54ea010d9e448fdb8e7a219345c7727
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Genetic background-dependent abnormalities of the enteric nervous system and intestinal function in Kif26a-deficient mice

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