Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England

Abstract Between April 2016 and September 2017, four cases of group B meningococcal disease were reported among sixth-form college students in Bristol, UK. Culture and non-culture whole genome sequencing was utilised and demonstrated that the four genomes of the responsible ST-41 strains clustered c...

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Autores principales:	Stephen A. Clark, Jay Lucidarme, Georgina Angel, Aiswarya Lekshmi, Begonia Morales-Aza, Laura Willerton, Helen Campbell, Steve J. Gray, Shamez N. Ladhani, Mike Wade, Mary Ramsay, Julie Yates, Adam Finn, Ray Borrow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e55372c68578427aa3b6547459be6eda
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spelling	oai:doaj.org-article:e55372c68578427aa3b6547459be6eda2021-12-02T15:08:08ZOutbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England10.1038/s41598-019-46483-32045-2322https://doaj.org/article/e55372c68578427aa3b6547459be6eda2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46483-3https://doaj.org/toc/2045-2322Abstract Between April 2016 and September 2017, four cases of group B meningococcal disease were reported among sixth-form college students in Bristol, UK. Culture and non-culture whole genome sequencing was utilised and demonstrated that the four genomes of the responsible ST-41 strains clustered closely on a sub-lineage of ST-41/44 clonal complex. The outbreak resulted in two fatalities. A distinct social group associated with one of the cases was selected for vaccination with 4CMenB and pharyngeal swabbing. In vitro culturing, multiple real-time PCR assays (sodC, ctrA and siaD B ) and a PorA PCR-sequencing assay were used to detect meningococcal colonisation and a carriage rate of 32.6% was observed. Furthermore, a high proportion of the pharyngeal swabs (78.3%) yielded a Factor H-Binding Protein (fHbp) nucleotide allele suggesting that the antigenic gene is prevalent among non-meningococcal flora, most likely Neisseria commensals. This may have implications for fHbp as a vaccine antigen should it be shown to influence bacterial colonisation.Stephen A. ClarkJay LucidarmeGeorgina AngelAiswarya LekshmiBegonia Morales-AzaLaura WillertonHelen CampbellSteve J. GrayShamez N. LadhaniMike WadeMary RamsayJulie YatesAdam FinnRay BorrowNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen A. Clark Jay Lucidarme Georgina Angel Aiswarya Lekshmi Begonia Morales-Aza Laura Willerton Helen Campbell Steve J. Gray Shamez N. Ladhani Mike Wade Mary Ramsay Julie Yates Adam Finn Ray Borrow Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
description	Abstract Between April 2016 and September 2017, four cases of group B meningococcal disease were reported among sixth-form college students in Bristol, UK. Culture and non-culture whole genome sequencing was utilised and demonstrated that the four genomes of the responsible ST-41 strains clustered closely on a sub-lineage of ST-41/44 clonal complex. The outbreak resulted in two fatalities. A distinct social group associated with one of the cases was selected for vaccination with 4CMenB and pharyngeal swabbing. In vitro culturing, multiple real-time PCR assays (sodC, ctrA and siaD B ) and a PorA PCR-sequencing assay were used to detect meningococcal colonisation and a carriage rate of 32.6% was observed. Furthermore, a high proportion of the pharyngeal swabs (78.3%) yielded a Factor H-Binding Protein (fHbp) nucleotide allele suggesting that the antigenic gene is prevalent among non-meningococcal flora, most likely Neisseria commensals. This may have implications for fHbp as a vaccine antigen should it be shown to influence bacterial colonisation.
format	article
author	Stephen A. Clark Jay Lucidarme Georgina Angel Aiswarya Lekshmi Begonia Morales-Aza Laura Willerton Helen Campbell Steve J. Gray Shamez N. Ladhani Mike Wade Mary Ramsay Julie Yates Adam Finn Ray Borrow
author_facet	Stephen A. Clark Jay Lucidarme Georgina Angel Aiswarya Lekshmi Begonia Morales-Aza Laura Willerton Helen Campbell Steve J. Gray Shamez N. Ladhani Mike Wade Mary Ramsay Julie Yates Adam Finn Ray Borrow
author_sort	Stephen A. Clark
title	Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
title_short	Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
title_full	Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
title_fullStr	Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
title_full_unstemmed	Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England
title_sort	outbreak strain characterisation and pharyngeal carriage detection following a protracted group b meningococcal outbreak in adolescents in south-west england
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e55372c68578427aa3b6547459be6eda
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Outbreak strain characterisation and pharyngeal carriage detection following a protracted group B meningococcal outbreak in adolescents in South-West England

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