Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii

Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad an...

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Autores principales:	Patrizia Lopalco, Julia Stahl, Cosimo Annese, Beate Averhoff, Angela Corcelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e558bcf545f6431e9423b20fce194b04
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spelling	oai:doaj.org-article:e558bcf545f6431e9423b20fce194b042021-12-02T11:41:00ZIdentification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii10.1038/s41598-017-03214-w2045-2322https://doaj.org/article/e558bcf545f6431e9423b20fce194b042017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03214-whttps://doaj.org/toc/2045-2322Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.Patrizia LopalcoJulia StahlCosimo AnneseBeate AverhoffAngela CorcelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
description	Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.
format	article
author	Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli
author_facet	Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli
author_sort	Patrizia Lopalco
title	Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
title_short	Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
title_full	Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
title_fullStr	Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
title_full_unstemmed	Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
title_sort	identification of unique cardiolipin and monolysocardiolipin species in acinetobacter baumannii
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e558bcf545f6431e9423b20fce194b04
work_keys_str_mv	AT patrizialopalco identificationofuniquecardiolipinandmonolysocardiolipinspeciesinacinetobacterbaumannii AT juliastahl identificationofuniquecardiolipinandmonolysocardiolipinspeciesinacinetobacterbaumannii AT cosimoannese identificationofuniquecardiolipinandmonolysocardiolipinspeciesinacinetobacterbaumannii AT beateaverhoff identificationofuniquecardiolipinandmonolysocardiolipinspeciesinacinetobacterbaumannii AT angelacorcelli identificationofuniquecardiolipinandmonolysocardiolipinspeciesinacinetobacterbaumannii
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Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii

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