Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.

Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poor...

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Autores principales:	Cihan Erkut, Andrej Vasilj, Sebastian Boland, Bianca Habermann, Andrej Shevchenko, Teymuras V Kurzchalia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e559e9186bf64438aa5663f81a39fb59
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spelling	oai:doaj.org-article:e559e9186bf64438aa5663f81a39fb592021-11-18T08:43:27ZMolecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.1932-620310.1371/journal.pone.0082473https://doaj.org/article/e559e9186bf64438aa5663f81a39fb592013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324795/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poorly understood. We recently showed that Caenorhabditis elegans dauer larva, an arrested stage specialized for survival in adverse conditions, is resistant to severe desiccation. However, this requires a preconditioning step at a mild desiccative environment to prepare the organism for harsher desiccation conditions. A systems approach was used to identify factors that are activated during this preconditioning. Using microarray analysis, proteomics, and bioinformatics, genes, proteins, and biochemical pathways that are upregulated during this process were identified. These pathways were validated via reverse genetics by testing the desiccation tolerances of mutants. These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons. This leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway. Remarkably, this response is specific and involves a small number of functional pathways, which represent the generic toolkit for anhydrobiosis in plants and animals.Cihan ErkutAndrej VasiljSebastian BolandBianca HabermannAndrej ShevchenkoTeymuras V KurzchaliaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82473 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cihan Erkut Andrej Vasilj Sebastian Boland Bianca Habermann Andrej Shevchenko Teymuras V Kurzchalia Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
description	Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poorly understood. We recently showed that Caenorhabditis elegans dauer larva, an arrested stage specialized for survival in adverse conditions, is resistant to severe desiccation. However, this requires a preconditioning step at a mild desiccative environment to prepare the organism for harsher desiccation conditions. A systems approach was used to identify factors that are activated during this preconditioning. Using microarray analysis, proteomics, and bioinformatics, genes, proteins, and biochemical pathways that are upregulated during this process were identified. These pathways were validated via reverse genetics by testing the desiccation tolerances of mutants. These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons. This leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway. Remarkably, this response is specific and involves a small number of functional pathways, which represent the generic toolkit for anhydrobiosis in plants and animals.
format	article
author	Cihan Erkut Andrej Vasilj Sebastian Boland Bianca Habermann Andrej Shevchenko Teymuras V Kurzchalia
author_facet	Cihan Erkut Andrej Vasilj Sebastian Boland Bianca Habermann Andrej Shevchenko Teymuras V Kurzchalia
author_sort	Cihan Erkut
title	Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
title_short	Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
title_full	Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
title_fullStr	Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
title_full_unstemmed	Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.
title_sort	molecular strategies of the caenorhabditis elegans dauer larva to survive extreme desiccation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e559e9186bf64438aa5663f81a39fb59
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Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation.

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