Comparative Determination of Melittin by Capillary Electrophoretic Methods

Bee venom from honey bees (Apis Mellifera L.) is known to have many pharmacological and biological properties. Melittin, a peptide consisting of 26 amino acids, is known as the main component of bee venom. The study aims to develop a rapid capillary electrophoresis method for separating and quantify...

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Autores principales:	Melda AKAY, Zeynep KALAYCIOĞLU, Sevgi KOLAYLI, Bedia BERKER
Formato:	article
Lenguaje:	EN
Publicado:	Turkish Chemical Society 2021
Materias:	apitherapy apitoxin melittin capillary electrophoresis bee venom Chemistry QD1-999
Acceso en línea:	https://doi.org/10.18596/jotcsa.949188 https://doaj.org/article/e55dd0a900994f6985d1b132403dd0ba
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spelling	oai:doaj.org-article:e55dd0a900994f6985d1b132403dd0ba2021-11-20T09:59:22Z Comparative Determination of Melittin by Capillary Electrophoretic Methods https://doi.org/10.18596/jotcsa.9491882149-0120https://doaj.org/article/e55dd0a900994f6985d1b132403dd0ba2021-11-01T00:00:00Zhttps://dergipark.org.tr/tr/pub/jotcsa/issue/64621/949188https://doaj.org/toc/2149-0120Bee venom from honey bees (Apis Mellifera L.) is known to have many pharmacological and biological properties. Melittin, a peptide consisting of 26 amino acids, is known as the main component of bee venom. The study aims to develop a rapid capillary electrophoresis method for separating and quantifying melittin in honeybee venom. Since melittin is a basic peptide, it will adhere to the capillary wall during separation. Two different methods were developed in this study for the capillary electrophoretic separation of melittin. As a first approach, a low pH buffer system was used. For the second approach, the capillary column was coated with a positively charged polymer (PEI). With both methods developed, the migration of melittin in the capillary was achieved by preventing wall adsorption. Melittin migrated in 6 min when the low-pH buffer system was applied, whereas its migration time is longer than 10 min in the PEI-coated capillary column. Thus, a low-pH buffer system was preferred for the analysis of the actual bee-venom sample. 100 mmol L-1 phosphoric acid/sodium dihydrogen phosphate system at pH 1.55 was chosen as separation buffer. As a conclusion, a fast and reliable method was developed for the determination of melittin in honeybee venom. The method was applied to an Anatolian bee venom sample to highlight the melittin amount. The melittin amount was found as 24.5 ± 3.4 g 100 g-1 in the bee venom sample.Melda AKAY Zeynep KALAYCIOĞLUSevgi KOLAYLIBedia BERKERTurkish Chemical Societyarticleapitherapyapitoxinmelittincapillary electrophoresisbee venomChemistryQD1-999ENJournal of the Turkish Chemical Society, Section A: Chemistry, Vol 8, Iss 4, Pp 1211 -1216 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apitherapy apitoxin melittin capillary electrophoresis bee venom Chemistry QD1-999
spellingShingle	apitherapy apitoxin melittin capillary electrophoresis bee venom Chemistry QD1-999 Melda AKAY Zeynep KALAYCIOĞLU Sevgi KOLAYLI Bedia BERKER Comparative Determination of Melittin by Capillary Electrophoretic Methods
description	Bee venom from honey bees (Apis Mellifera L.) is known to have many pharmacological and biological properties. Melittin, a peptide consisting of 26 amino acids, is known as the main component of bee venom. The study aims to develop a rapid capillary electrophoresis method for separating and quantifying melittin in honeybee venom. Since melittin is a basic peptide, it will adhere to the capillary wall during separation. Two different methods were developed in this study for the capillary electrophoretic separation of melittin. As a first approach, a low pH buffer system was used. For the second approach, the capillary column was coated with a positively charged polymer (PEI). With both methods developed, the migration of melittin in the capillary was achieved by preventing wall adsorption. Melittin migrated in 6 min when the low-pH buffer system was applied, whereas its migration time is longer than 10 min in the PEI-coated capillary column. Thus, a low-pH buffer system was preferred for the analysis of the actual bee-venom sample. 100 mmol L-1 phosphoric acid/sodium dihydrogen phosphate system at pH 1.55 was chosen as separation buffer. As a conclusion, a fast and reliable method was developed for the determination of melittin in honeybee venom. The method was applied to an Anatolian bee venom sample to highlight the melittin amount. The melittin amount was found as 24.5 ± 3.4 g 100 g-1 in the bee venom sample.
format	article
author	Melda AKAY Zeynep KALAYCIOĞLU Sevgi KOLAYLI Bedia BERKER
author_facet	Melda AKAY Zeynep KALAYCIOĞLU Sevgi KOLAYLI Bedia BERKER
author_sort	Melda AKAY
title	Comparative Determination of Melittin by Capillary Electrophoretic Methods
title_short	Comparative Determination of Melittin by Capillary Electrophoretic Methods
title_full	Comparative Determination of Melittin by Capillary Electrophoretic Methods
title_fullStr	Comparative Determination of Melittin by Capillary Electrophoretic Methods
title_full_unstemmed	Comparative Determination of Melittin by Capillary Electrophoretic Methods
title_sort	comparative determination of melittin by capillary electrophoretic methods
publisher	Turkish Chemical Society
publishDate	2021
url	https://doi.org/10.18596/jotcsa.949188 https://doaj.org/article/e55dd0a900994f6985d1b132403dd0ba
work_keys_str_mv	AT meldaakay comparativedeterminationofmelittinbycapillaryelectrophoreticmethods AT zeynepkalaycioglu comparativedeterminationofmelittinbycapillaryelectrophoreticmethods AT sevgikolayli comparativedeterminationofmelittinbycapillaryelectrophoreticmethods AT bediaberker comparativedeterminationofmelittinbycapillaryelectrophoreticmethods
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Comparative Determination of Melittin by Capillary Electrophoretic Methods

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