Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

Abstract The preservation of nucleic acids from clinical samples is critical to facilitate accurate molecular diagnosis. The use of a paper matrix, Flinders Technology Associates (FTA) Elute cards, to archive DNA and viral RNA is well-documented. However, the feasibility of FTA Elute cards for archi...

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Autores principales:	Shu Hui Neo, Ka Yan Chung, Jia Min Quek, Heng-Phon Too
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e55f35a87faf420496f03718b7a725fa
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spelling	oai:doaj.org-article:e55f35a87faf420496f03718b7a725fa2021-12-02T15:05:35ZTrehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix10.1038/s41598-017-16960-82045-2322https://doaj.org/article/e55f35a87faf420496f03718b7a725fa2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16960-8https://doaj.org/toc/2045-2322Abstract The preservation of nucleic acids from clinical samples is critical to facilitate accurate molecular diagnosis. The use of a paper matrix, Flinders Technology Associates (FTA) Elute cards, to archive DNA and viral RNA is well-documented. However, the feasibility of FTA Elute cards for archiving serum and serum exosomal microRNAs (miRNAs) remains unclear. Here, we performed a comprehensive evaluation of FTA Elute cards for miRNA storage and recovery in different pre-analytical conditions. The recovery of serum miRNA dry-spotted on FTA Elute cards by direct elution with water at high temperature was poor. However, serum miRNAs dry-spotted on the cards were isolated with about 40% yield when using QIAzol lysis reagent and recovery was improved remarkably (>80%) upon extraction from cards pre-treated with trehalose. miRNAs stored on the cards remained stable at room temperature and can be kept for prolonged periods. Furthermore, miRNAs could be similarly recovered from serum exosomes dry-spotted on the cards. Importantly, when using sera from gastric cancer (GC) patients, the miRNAs were efficiently recovered from trehalose pre-treated cards without affecting their representation. Collectively, we have demonstrated the potential of FTA Elute cards to archive serum and serum exosomal miRNAs, making it useful for biomarker discovery and diagnostics.Shu Hui NeoKa Yan ChungJia Min QuekHeng-Phon TooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu Hui Neo Ka Yan Chung Jia Min Quek Heng-Phon Too Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
description	Abstract The preservation of nucleic acids from clinical samples is critical to facilitate accurate molecular diagnosis. The use of a paper matrix, Flinders Technology Associates (FTA) Elute cards, to archive DNA and viral RNA is well-documented. However, the feasibility of FTA Elute cards for archiving serum and serum exosomal microRNAs (miRNAs) remains unclear. Here, we performed a comprehensive evaluation of FTA Elute cards for miRNA storage and recovery in different pre-analytical conditions. The recovery of serum miRNA dry-spotted on FTA Elute cards by direct elution with water at high temperature was poor. However, serum miRNAs dry-spotted on the cards were isolated with about 40% yield when using QIAzol lysis reagent and recovery was improved remarkably (>80%) upon extraction from cards pre-treated with trehalose. miRNAs stored on the cards remained stable at room temperature and can be kept for prolonged periods. Furthermore, miRNAs could be similarly recovered from serum exosomes dry-spotted on the cards. Importantly, when using sera from gastric cancer (GC) patients, the miRNAs were efficiently recovered from trehalose pre-treated cards without affecting their representation. Collectively, we have demonstrated the potential of FTA Elute cards to archive serum and serum exosomal miRNAs, making it useful for biomarker discovery and diagnostics.
format	article
author	Shu Hui Neo Ka Yan Chung Jia Min Quek Heng-Phon Too
author_facet	Shu Hui Neo Ka Yan Chung Jia Min Quek Heng-Phon Too
author_sort	Shu Hui Neo
title	Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
title_short	Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
title_full	Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
title_fullStr	Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
title_full_unstemmed	Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix
title_sort	trehalose significantly enhances the recovery of serum and serum exosomal mirna from a paper-based matrix
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e55f35a87faf420496f03718b7a725fa
work_keys_str_mv	AT shuhuineo trehalosesignificantlyenhancestherecoveryofserumandserumexosomalmirnafromapaperbasedmatrix AT kayanchung trehalosesignificantlyenhancestherecoveryofserumandserumexosomalmirnafromapaperbasedmatrix AT jiaminquek trehalosesignificantlyenhancestherecoveryofserumandserumexosomalmirnafromapaperbasedmatrix AT hengphontoo trehalosesignificantlyenhancestherecoveryofserumandserumexosomalmirnafromapaperbasedmatrix
_version_	1718388800312311808

Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

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