Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA

Abstract Recognizing new anticancer compounds to improve Breast cancer treatment seems crucial. Essential oil of Zataria Multiflora (ZEO) is a secondary metabolite with some biological properties, yet underlying cellular and molecular anticancer properties of ZEO is unclear. GC/MS analysis revealed...

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Autores principales:	Fahimeh Salehi, Hossein Behboudi, Gholamreza Kavoosi, Sussan K. Ardestani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e56038bc970c4b50a12745a557059105
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spelling	oai:doaj.org-article:e56038bc970c4b50a12745a5570591052021-12-02T12:32:30ZMonitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA10.1038/s41598-017-02633-z2045-2322https://doaj.org/article/e56038bc970c4b50a12745a5570591052017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02633-zhttps://doaj.org/toc/2045-2322Abstract Recognizing new anticancer compounds to improve Breast cancer treatment seems crucial. Essential oil of Zataria Multiflora (ZEO) is a secondary metabolite with some biological properties, yet underlying cellular and molecular anticancer properties of ZEO is unclear. GC/MS analysis revealed that carvacrol is the major ingredient of the essential oil. ZEO increasingly suppressed viability in MDA-MB-231, MCF-7 and T47D Breast cancer cells while nontoxic to L929 normal cells in monolayer cell cultures (2D), whereas MDA-MB-231 multicellular spheroids (3D) were more resistant to inhibition. ZEO significantly induced cell apoptosis confirmed by fluorescent staining, flow cytometry analysis and DNA fragmentation in MDA-MB-231 2D and 3D cell cultures. ZEO increased ROS generation and subsequent loss of ΔΨm, caspase 3 activation and DNA damage which consequently caused G1 and G2/M cell cycle arrest in a dose- and time-dependent manner in 2D. S phase arrest occurred in cell spheroids therefore ZEO possible DNA interaction with gDNA was investigated and revealed ZEO binds DNA via intercalation. Altogether, these data corroborate anticancer properties of ZEO and suggest that cell culture format (2D monolayer vs. 3D spheroid) plays a critical role in drug response and provides new insights into the mechanisms underlying ZEO cytotoxicity effect on Breast cancer cells.Fahimeh SalehiHossein BehboudiGholamreza KavoosiSussan K. ArdestaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fahimeh Salehi Hossein Behboudi Gholamreza Kavoosi Sussan K. Ardestani Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
description	Abstract Recognizing new anticancer compounds to improve Breast cancer treatment seems crucial. Essential oil of Zataria Multiflora (ZEO) is a secondary metabolite with some biological properties, yet underlying cellular and molecular anticancer properties of ZEO is unclear. GC/MS analysis revealed that carvacrol is the major ingredient of the essential oil. ZEO increasingly suppressed viability in MDA-MB-231, MCF-7 and T47D Breast cancer cells while nontoxic to L929 normal cells in monolayer cell cultures (2D), whereas MDA-MB-231 multicellular spheroids (3D) were more resistant to inhibition. ZEO significantly induced cell apoptosis confirmed by fluorescent staining, flow cytometry analysis and DNA fragmentation in MDA-MB-231 2D and 3D cell cultures. ZEO increased ROS generation and subsequent loss of ΔΨm, caspase 3 activation and DNA damage which consequently caused G1 and G2/M cell cycle arrest in a dose- and time-dependent manner in 2D. S phase arrest occurred in cell spheroids therefore ZEO possible DNA interaction with gDNA was investigated and revealed ZEO binds DNA via intercalation. Altogether, these data corroborate anticancer properties of ZEO and suggest that cell culture format (2D monolayer vs. 3D spheroid) plays a critical role in drug response and provides new insights into the mechanisms underlying ZEO cytotoxicity effect on Breast cancer cells.
format	article
author	Fahimeh Salehi Hossein Behboudi Gholamreza Kavoosi Sussan K. Ardestani
author_facet	Fahimeh Salehi Hossein Behboudi Gholamreza Kavoosi Sussan K. Ardestani
author_sort	Fahimeh Salehi
title	Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
title_short	Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
title_full	Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
title_fullStr	Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
title_full_unstemmed	Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA
title_sort	monitoring zeo apoptotic potential in 2d and 3d cell cultures and associated spectroscopic evidence on mode of interaction with dna
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e56038bc970c4b50a12745a557059105
work_keys_str_mv	AT fahimehsalehi monitoringzeoapoptoticpotentialin2dand3dcellculturesandassociatedspectroscopicevidenceonmodeofinteractionwithdna AT hosseinbehboudi monitoringzeoapoptoticpotentialin2dand3dcellculturesandassociatedspectroscopicevidenceonmodeofinteractionwithdna AT gholamrezakavoosi monitoringzeoapoptoticpotentialin2dand3dcellculturesandassociatedspectroscopicevidenceonmodeofinteractionwithdna AT sussankardestani monitoringzeoapoptoticpotentialin2dand3dcellculturesandassociatedspectroscopicevidenceonmodeofinteractionwithdna
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Monitoring ZEO apoptotic potential in 2D and 3D cell cultures and associated spectroscopic evidence on mode of interaction with DNA

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