Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand
Abstract Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Ca...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e561be68a4d74b6890b162a3ab5db4ce |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e561be68a4d74b6890b162a3ab5db4ce |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e561be68a4d74b6890b162a3ab5db4ce2021-12-02T18:18:44ZPlasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand10.1038/s41598-021-92735-62045-2322https://doaj.org/article/e561be68a4d74b6890b162a3ab5db4ce2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92735-6https://doaj.org/toc/2045-2322Abstract Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.Nonlawat BoonyalaiChatchadaporn ThamnurakPiyaporn Sai-ngamWinita Ta-aksornMontri ArsanokNichapat UthaimongkolSiratchana SundrakesSorayut ChattrakarnChaiyaporn ChaisatitChantida PraditpolWatcharintorn FagnarkKirakarn KirativanichSuwanna ChaorattanakaweePattaraporn VanachayangkulPaphavee LertsethtakarnPanita GosiDarunee UtainnamWuttikon RodkvamtookWorachet KuntawunginnBrian A. VeselyMichele D. SpringMark M. FukudaCharlotte LanteriDouglas WalshDavid L. SaundersPhilip L. SmithMariusz WojnarskiNarongrid SirisopanaNorman C. WatersKrisada JongsakulJariyanart GayweeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Nonlawat Boonyalai Chatchadaporn Thamnurak Piyaporn Sai-ngam Winita Ta-aksorn Montri Arsanok Nichapat Uthaimongkol Siratchana Sundrakes Sorayut Chattrakarn Chaiyaporn Chaisatit Chantida Praditpol Watcharintorn Fagnark Kirakarn Kirativanich Suwanna Chaorattanakawee Pattaraporn Vanachayangkul Paphavee Lertsethtakarn Panita Gosi Darunee Utainnam Wuttikon Rodkvamtook Worachet Kuntawunginn Brian A. Vesely Michele D. Spring Mark M. Fukuda Charlotte Lanteri Douglas Walsh David L. Saunders Philip L. Smith Mariusz Wojnarski Narongrid Sirisopana Norman C. Waters Krisada Jongsakul Jariyanart Gaywee Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| description |
Abstract Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions. |
| format |
article |
| author |
Nonlawat Boonyalai Chatchadaporn Thamnurak Piyaporn Sai-ngam Winita Ta-aksorn Montri Arsanok Nichapat Uthaimongkol Siratchana Sundrakes Sorayut Chattrakarn Chaiyaporn Chaisatit Chantida Praditpol Watcharintorn Fagnark Kirakarn Kirativanich Suwanna Chaorattanakawee Pattaraporn Vanachayangkul Paphavee Lertsethtakarn Panita Gosi Darunee Utainnam Wuttikon Rodkvamtook Worachet Kuntawunginn Brian A. Vesely Michele D. Spring Mark M. Fukuda Charlotte Lanteri Douglas Walsh David L. Saunders Philip L. Smith Mariusz Wojnarski Narongrid Sirisopana Norman C. Waters Krisada Jongsakul Jariyanart Gaywee |
| author_facet |
Nonlawat Boonyalai Chatchadaporn Thamnurak Piyaporn Sai-ngam Winita Ta-aksorn Montri Arsanok Nichapat Uthaimongkol Siratchana Sundrakes Sorayut Chattrakarn Chaiyaporn Chaisatit Chantida Praditpol Watcharintorn Fagnark Kirakarn Kirativanich Suwanna Chaorattanakawee Pattaraporn Vanachayangkul Paphavee Lertsethtakarn Panita Gosi Darunee Utainnam Wuttikon Rodkvamtook Worachet Kuntawunginn Brian A. Vesely Michele D. Spring Mark M. Fukuda Charlotte Lanteri Douglas Walsh David L. Saunders Philip L. Smith Mariusz Wojnarski Narongrid Sirisopana Norman C. Waters Krisada Jongsakul Jariyanart Gaywee |
| author_sort |
Nonlawat Boonyalai |
| title |
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| title_short |
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| title_full |
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| title_fullStr |
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| title_full_unstemmed |
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand |
| title_sort |
plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of piperaquine resistance in northeastern thailand |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e561be68a4d74b6890b162a3ab5db4ce |
| work_keys_str_mv |
AT nonlawatboonyalai plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT chatchadapornthamnurak plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT piyapornsaingam plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT winitataaksorn plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT montriarsanok plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT nichapatuthaimongkol plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT siratchanasundrakes plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT sorayutchattrakarn plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT chaiyapornchaisatit plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT chantidapraditpol plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT watcharintornfagnark plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT kirakarnkirativanich plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT suwannachaorattanakawee plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT pattarapornvanachayangkul plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT paphaveelertsethtakarn plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT panitagosi plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT daruneeutainnam plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT wuttikonrodkvamtook plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT worachetkuntawunginn plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT brianavesely plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT micheledspring plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT markmfukuda plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT charlottelanteri plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT douglaswalsh plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT davidlsaunders plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT philiplsmith plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT mariuszwojnarski plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT narongridsirisopana plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT normancwaters plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT krisadajongsakul plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand AT jariyanartgaywee plasmodiumfalciparumphenotypicandgenotypicresistanceprofileduringtheemergenceofpiperaquineresistanceinnortheasternthailand |
| _version_ |
1718378248710127616 |