Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.

Wolbachia is a bacteria endosymbiont that rapidly infects insect populations through a mechanism known as cytoplasmic incompatibility (CI). In CI, crosses between Wolbachia-infected males and uninfected females produce severe cell cycle defects in the male pronucleus resulting in early embryonic let...

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Autores principales:	Frédéric Landmann, Guillermo A Orsi, Benjamin Loppin, William Sullivan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e5636bb2b5a8438f864bdb77529792ad
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spelling	oai:doaj.org-article:e5636bb2b5a8438f864bdb77529792ad2021-11-25T05:47:11ZWolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.1553-73661553-737410.1371/journal.ppat.1000343https://doaj.org/article/e5636bb2b5a8438f864bdb77529792ad2009-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19300496/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Wolbachia is a bacteria endosymbiont that rapidly infects insect populations through a mechanism known as cytoplasmic incompatibility (CI). In CI, crosses between Wolbachia-infected males and uninfected females produce severe cell cycle defects in the male pronucleus resulting in early embryonic lethality. In contrast, viable progeny are produced when both parents are infected (the Rescue cross). An important consequence of CI-Rescue is that infected females have a selective advantage over uninfected females facilitating the rapid spread of Wolbachia through insect populations. CI disrupts a number of prophase and metaphase events in the male pronucleus, including Cdk1 activation, chromosome condensation, and segregation. Here, we demonstrate that CI disrupts earlier interphase cell cycle events. Specifically, CI delays the H3.3 and H4 deposition that occurs immediately after protamine removal from the male pronucleus. In addition, we find prolonged retention of the replication factor PCNA in the male pronucleus into metaphase, indicating progression into mitosis with incompletely replicated DNA. We propose that these CI-induced interphase defects in de novo nucleosome assembly and replication are the cause of the observed mitotic condensation and segregation defects. In addition, these interphase chromosome defects likely activate S-phase checkpoints, accounting for the previously described delays in Cdk1 activation. These results have important implications for the mechanism of Rescue and other Wolbachia-induced phenotypes.Frédéric LandmannGuillermo A OrsiBenjamin LoppinWilliam SullivanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 3, p e1000343 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Frédéric Landmann Guillermo A Orsi Benjamin Loppin William Sullivan Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
description	Wolbachia is a bacteria endosymbiont that rapidly infects insect populations through a mechanism known as cytoplasmic incompatibility (CI). In CI, crosses between Wolbachia-infected males and uninfected females produce severe cell cycle defects in the male pronucleus resulting in early embryonic lethality. In contrast, viable progeny are produced when both parents are infected (the Rescue cross). An important consequence of CI-Rescue is that infected females have a selective advantage over uninfected females facilitating the rapid spread of Wolbachia through insect populations. CI disrupts a number of prophase and metaphase events in the male pronucleus, including Cdk1 activation, chromosome condensation, and segregation. Here, we demonstrate that CI disrupts earlier interphase cell cycle events. Specifically, CI delays the H3.3 and H4 deposition that occurs immediately after protamine removal from the male pronucleus. In addition, we find prolonged retention of the replication factor PCNA in the male pronucleus into metaphase, indicating progression into mitosis with incompletely replicated DNA. We propose that these CI-induced interphase defects in de novo nucleosome assembly and replication are the cause of the observed mitotic condensation and segregation defects. In addition, these interphase chromosome defects likely activate S-phase checkpoints, accounting for the previously described delays in Cdk1 activation. These results have important implications for the mechanism of Rescue and other Wolbachia-induced phenotypes.
format	article
author	Frédéric Landmann Guillermo A Orsi Benjamin Loppin William Sullivan
author_facet	Frédéric Landmann Guillermo A Orsi Benjamin Loppin William Sullivan
author_sort	Frédéric Landmann
title	Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
title_short	Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
title_full	Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
title_fullStr	Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
title_full_unstemmed	Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
title_sort	wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/e5636bb2b5a8438f864bdb77529792ad
work_keys_str_mv	AT fredericlandmann wolbachiamediatedcytoplasmicincompatibilityisassociatedwithimpairedhistonedepositioninthemalepronucleus AT guillermoaorsi wolbachiamediatedcytoplasmicincompatibilityisassociatedwithimpairedhistonedepositioninthemalepronucleus AT benjaminloppin wolbachiamediatedcytoplasmicincompatibilityisassociatedwithimpairedhistonedepositioninthemalepronucleus AT williamsullivan wolbachiamediatedcytoplasmicincompatibilityisassociatedwithimpairedhistonedepositioninthemalepronucleus
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Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus.

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