Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat

The presence of awns on the ear is associated with a number of important plant properties, such as drought resistance, quality of the grain mass during processing, etc. The main manifestations of this trait are controlled by the <i>B1</i> gene, which has recently been identified and enco...

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Autores principales:	Andrey B. Shcherban, Diana D. Kuvaeva, Olga P. Mitrofanova, Svetlana E. Khverenets, Alexander I. Pryanishnikov, Elena A. Salina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ear cultivar C2H2 zinc finger haplotype marker <i>B1</i> locus Botany QK1-989
Acceso en línea:	https://doaj.org/article/e57edd8f2f304d32a9e48da1151a5930
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spelling	oai:doaj.org-article:e57edd8f2f304d32a9e48da1151a59302021-11-25T18:44:47ZTargeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat10.3390/plants101122852223-7747https://doaj.org/article/e57edd8f2f304d32a9e48da1151a59302021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2285https://doaj.org/toc/2223-7747The presence of awns on the ear is associated with a number of important plant properties, such as drought resistance, quality of the grain mass during processing, etc. The main manifestations of this trait are controlled by the <i>B1</i> gene, which has recently been identified and encodes the C2H2 zinc finger transcription factor. Based on the previously identified SNPs in the promoter region of this gene, we constructed markers for dominant and recessive alleles which determine awnless and awned phenotypes, respectively. The markers were successful for use in targeting the respective alleles of the <i>B1</i> gene in 176 varieties of common wheat, accessions of <i>T. spelta</i> L., as well as on F2/F3 hybrids from crosses between awned and awnless forms of <i>T. aestivum</i>. We first identified a new allele, <i>b1mite</i>, which has both an insert of a miniature Stowaway-like transposon, 261 bp in length, and 33 novel SNPs in the promoter region. Despite these changes, this allele had no effect on the awned phenotype. The possible mechanisms of the influence of the analyzed gene on phenotype are discussed.Andrey B. ShcherbanDiana D. KuvaevaOlga P. MitrofanovaSvetlana E. KhverenetsAlexander I. PryanishnikovElena A. SalinaMDPI AGarticleearcultivarC2H2 zinc fingerhaplotypemarker<i>B1</i> locusBotanyQK1-989ENPlants, Vol 10, Iss 2285, p 2285 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ear cultivar C2H2 zinc finger haplotype marker <i>B1</i> locus Botany QK1-989
spellingShingle	ear cultivar C2H2 zinc finger haplotype marker <i>B1</i> locus Botany QK1-989 Andrey B. Shcherban Diana D. Kuvaeva Olga P. Mitrofanova Svetlana E. Khverenets Alexander I. Pryanishnikov Elena A. Salina Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
description	The presence of awns on the ear is associated with a number of important plant properties, such as drought resistance, quality of the grain mass during processing, etc. The main manifestations of this trait are controlled by the <i>B1</i> gene, which has recently been identified and encodes the C2H2 zinc finger transcription factor. Based on the previously identified SNPs in the promoter region of this gene, we constructed markers for dominant and recessive alleles which determine awnless and awned phenotypes, respectively. The markers were successful for use in targeting the respective alleles of the <i>B1</i> gene in 176 varieties of common wheat, accessions of <i>T. spelta</i> L., as well as on F2/F3 hybrids from crosses between awned and awnless forms of <i>T. aestivum</i>. We first identified a new allele, <i>b1mite</i>, which has both an insert of a miniature Stowaway-like transposon, 261 bp in length, and 33 novel SNPs in the promoter region. Despite these changes, this allele had no effect on the awned phenotype. The possible mechanisms of the influence of the analyzed gene on phenotype are discussed.
format	article
author	Andrey B. Shcherban Diana D. Kuvaeva Olga P. Mitrofanova Svetlana E. Khverenets Alexander I. Pryanishnikov Elena A. Salina
author_facet	Andrey B. Shcherban Diana D. Kuvaeva Olga P. Mitrofanova Svetlana E. Khverenets Alexander I. Pryanishnikov Elena A. Salina
author_sort	Andrey B. Shcherban
title	Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
title_short	Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
title_full	Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
title_fullStr	Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
title_full_unstemmed	Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat
title_sort	targeting the <i>b1</i> gene and analysis of its polymorphism associated with awned/awnless trait in russian germplasm collections of common wheat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e57edd8f2f304d32a9e48da1151a5930
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Targeting the <i>B1</i> Gene and Analysis of Its Polymorphism Associated with Awned/Awnless Trait in Russian Germplasm Collections of Common Wheat

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