DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.

DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of r...

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Autores principales:	Kosuke Nozawa, Jiani Chen, Jianjun Jiang, Sarah M Leichter, Masataka Yamada, Takamasa Suzuki, Fengquan Liu, Hidetaka Ito, Xuehua Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/e589987b8b8a418b8c5fb9c2002ee84a
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spelling	oai:doaj.org-article:e589987b8b8a418b8c5fb9c2002ee84a2021-12-02T20:03:23ZDNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.1553-73901553-740410.1371/journal.pgen.1009710https://doaj.org/article/e589987b8b8a418b8c5fb9c2002ee84a2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009710https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of retrotransposon ONSEN. We found that the full transcription of ONSEN under heat stress requires CMT3. Interestingly, loss-of-function CMT3 mutation led to increased CHH methylation at ONSEN. The CHH methylation is mediated by CMT2, as evidenced by greatly reduced CHH methylation in cmt2 and cmt2 cmt3 mutants coupled with increased ONSEN transcription. Furthermore, we found more CMT2 binding at ONSEN chromatin in cmt3 compared to wild-type accompanied with an ectopic accumulation of H3K9me2 under heat stress, suggesting a collaborative role of H3K9me2 and CHH methylation in preventing heat-induced ONSEN activation. In summary, this study identifies a non-canonical role of CMT3 in preventing transposon silencing and provides new insights into how DNA methyltransferases regulate transcription under stress conditions.Kosuke NozawaJiani ChenJianjun JiangSarah M LeichterMasataka YamadaTakamasa SuzukiFengquan LiuHidetaka ItoXuehua ZhongPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009710 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Kosuke Nozawa Jiani Chen Jianjun Jiang Sarah M Leichter Masataka Yamada Takamasa Suzuki Fengquan Liu Hidetaka Ito Xuehua Zhong DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
description	DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of retrotransposon ONSEN. We found that the full transcription of ONSEN under heat stress requires CMT3. Interestingly, loss-of-function CMT3 mutation led to increased CHH methylation at ONSEN. The CHH methylation is mediated by CMT2, as evidenced by greatly reduced CHH methylation in cmt2 and cmt2 cmt3 mutants coupled with increased ONSEN transcription. Furthermore, we found more CMT2 binding at ONSEN chromatin in cmt3 compared to wild-type accompanied with an ectopic accumulation of H3K9me2 under heat stress, suggesting a collaborative role of H3K9me2 and CHH methylation in preventing heat-induced ONSEN activation. In summary, this study identifies a non-canonical role of CMT3 in preventing transposon silencing and provides new insights into how DNA methyltransferases regulate transcription under stress conditions.
format	article
author	Kosuke Nozawa Jiani Chen Jianjun Jiang Sarah M Leichter Masataka Yamada Takamasa Suzuki Fengquan Liu Hidetaka Ito Xuehua Zhong
author_facet	Kosuke Nozawa Jiani Chen Jianjun Jiang Sarah M Leichter Masataka Yamada Takamasa Suzuki Fengquan Liu Hidetaka Ito Xuehua Zhong
author_sort	Kosuke Nozawa
title	DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
title_short	DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
title_full	DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
title_fullStr	DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
title_full_unstemmed	DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.
title_sort	dna methyltransferase chromomethylase3 prevents onsen transposon silencing under heat stress.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e589987b8b8a418b8c5fb9c2002ee84a
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DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress.

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