Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines

Perillaldehyde (PAH), one of the active ingredients of the traditional Chinese medicine (TCM) plant Perilla frutescens, is widely used and exerts crucial anti-cancer activities. The aim of current study is to illustrate the potential mechanisms of PAH-mediated regulation of bone metastasis and osteo...

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Autores principales:	Zhuoyuan Lin, Sheng Huang, Xitao LingHu, Yixiao Wang, Bin Wang, Shaowen Zhong, Shangyan Xie, Xiaohong Xu, Aorigele yu, Atsushi Nagai, Yuta Kobayashi, Qingde Wa, Shuai Huang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e590dde4ebed440da1c7ae962890e549
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spelling	oai:doaj.org-article:e590dde4ebed440da1c7ae962890e5492021-11-11T14:23:43ZPerillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines2165-59792165-598710.1080/21655979.2021.2001237https://doaj.org/article/e590dde4ebed440da1c7ae962890e5492021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001237https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Perillaldehyde (PAH), one of the active ingredients of the traditional Chinese medicine (TCM) plant Perilla frutescens, is widely used and exerts crucial anti-cancer activities. The aim of current study is to illustrate the potential mechanisms of PAH-mediated regulation of bone metastasis and osteoclastogenesis in prostate cancer (PCa) cell lines. Effects of PAH on proliferation, invasion and migration of PC-3 cells were assessed with the Cell Counting Kit-8 (CCK-8) assay and Transwell assays, respectively. Effects of PAH on stem cell characteristics of PC-3 cells were evaluated by cell-matrix adhesion assay, colony formation assay, spheroid formation assay, as well as western blot . The anti-metastasis and anti-osteoclastogenesis activity of PAH in RAW264.7 cells was examined by osteoclast differentiation assay and western blot. The protein levels of CD133 and CD44 in PC-3 cells and the activity of nuclear factor kappa B (NF-κB) signaling pathway in RAW264.7 cells were measured by western blot. PAH suppressed proliferation, invasion and migration of PC-3 cells, prevented stem cell characteristics including cell-matrix adhesion, colony formation, spheroid formation as well as CD133 and CD44 expression. PAH inhibited bone metastasis and osteoclastogenesis via repressing the activation of NF-κB pathway as well as (RANKL)- and cancer cell- induced osteoclastogenesis in PCa cells. These findings suggested the potential therapeutic effects of PAH on the metastasis of patients with PCa.Zhuoyuan LinSheng HuangXitao LingHuYixiao WangBin WangShaowen ZhongShangyan XieXiaohong XuAorigele yuAtsushi NagaiYuta KobayashiQingde WaShuai HuangTaylor & Francis GrouparticleBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Zhuoyuan Lin Sheng Huang Xitao LingHu Yixiao Wang Bin Wang Shaowen Zhong Shangyan Xie Xiaohong Xu Aorigele yu Atsushi Nagai Yuta Kobayashi Qingde Wa Shuai Huang Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
description	Perillaldehyde (PAH), one of the active ingredients of the traditional Chinese medicine (TCM) plant Perilla frutescens, is widely used and exerts crucial anti-cancer activities. The aim of current study is to illustrate the potential mechanisms of PAH-mediated regulation of bone metastasis and osteoclastogenesis in prostate cancer (PCa) cell lines. Effects of PAH on proliferation, invasion and migration of PC-3 cells were assessed with the Cell Counting Kit-8 (CCK-8) assay and Transwell assays, respectively. Effects of PAH on stem cell characteristics of PC-3 cells were evaluated by cell-matrix adhesion assay, colony formation assay, spheroid formation assay, as well as western blot . The anti-metastasis and anti-osteoclastogenesis activity of PAH in RAW264.7 cells was examined by osteoclast differentiation assay and western blot. The protein levels of CD133 and CD44 in PC-3 cells and the activity of nuclear factor kappa B (NF-κB) signaling pathway in RAW264.7 cells were measured by western blot. PAH suppressed proliferation, invasion and migration of PC-3 cells, prevented stem cell characteristics including cell-matrix adhesion, colony formation, spheroid formation as well as CD133 and CD44 expression. PAH inhibited bone metastasis and osteoclastogenesis via repressing the activation of NF-κB pathway as well as (RANKL)- and cancer cell- induced osteoclastogenesis in PCa cells. These findings suggested the potential therapeutic effects of PAH on the metastasis of patients with PCa.
format	article
author	Zhuoyuan Lin Sheng Huang Xitao LingHu Yixiao Wang Bin Wang Shaowen Zhong Shangyan Xie Xiaohong Xu Aorigele yu Atsushi Nagai Yuta Kobayashi Qingde Wa Shuai Huang
author_facet	Zhuoyuan Lin Sheng Huang Xitao LingHu Yixiao Wang Bin Wang Shaowen Zhong Shangyan Xie Xiaohong Xu Aorigele yu Atsushi Nagai Yuta Kobayashi Qingde Wa Shuai Huang
author_sort	Zhuoyuan Lin
title	Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
title_short	Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
title_full	Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
title_fullStr	Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
title_full_unstemmed	Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines
title_sort	perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κb ligand (rankl) signaling-induced osteoclastogenesis in prostate cancer cell lines
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/e590dde4ebed440da1c7ae962890e549
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Perillaldehyde inhibits bone metastasis and receptor activator of nuclear factor-κB ligand (RANKL) signaling-induced osteoclastogenesis in prostate cancer cell lines

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