First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages

Background Agro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential. Methods Twelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experimen...

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Autores principales:	Jannette Wen Fang Wu Wu, Mauricio Redondo-Solano, Lidieth Uribe, Rodolfo WingChing-Jones, Jessie Usaga, Natalia Barboza
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Agro-industrial residuals Gastrointestinal tract survivor Pathogens Inhibition Antibiotics resistance Medicine R
Acceso en línea:	https://doaj.org/article/e5926cf25f684ecbae0cafe4d6be6435
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spelling	oai:doaj.org-article:e5926cf25f684ecbae0cafe4d6be64352021-12-02T15:05:09ZFirst characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages10.7717/peerj.124372167-8359https://doaj.org/article/e5926cf25f684ecbae0cafe4d6be64352021-11-01T00:00:00Zhttps://peerj.com/articles/12437.pdfhttps://peerj.com/articles/12437/https://doaj.org/toc/2167-8359Background Agro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential. Methods Twelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells. Results Lacticaseibacillus paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and Weissella ghanensis were identified. Survival of one of the isolates was 90% or higher after exposure to acidic conditions (pH: 2), six isolates showed at least 61% survival after exposure to bile salts. The three most promising isolates, based on survivability tests, showed a strong antagonistic effect against Salmonella. However, only L. paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it also showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin.Jannette Wen Fang Wu WuMauricio Redondo-SolanoLidieth UribeRodolfo WingChing-JonesJessie UsagaNatalia BarbozaPeerJ Inc.articleAgro-industrial residualsGastrointestinal tract survivorPathogensInhibitionAntibiotics resistanceMedicineRENPeerJ, Vol 9, p e12437 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Agro-industrial residuals Gastrointestinal tract survivor Pathogens Inhibition Antibiotics resistance Medicine R
spellingShingle	Agro-industrial residuals Gastrointestinal tract survivor Pathogens Inhibition Antibiotics resistance Medicine R Jannette Wen Fang Wu Wu Mauricio Redondo-Solano Lidieth Uribe Rodolfo WingChing-Jones Jessie Usaga Natalia Barboza First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
description	Background Agro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential. Methods Twelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells. Results Lacticaseibacillus paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and Weissella ghanensis were identified. Survival of one of the isolates was 90% or higher after exposure to acidic conditions (pH: 2), six isolates showed at least 61% survival after exposure to bile salts. The three most promising isolates, based on survivability tests, showed a strong antagonistic effect against Salmonella. However, only L. paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it also showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origin.
format	article
author	Jannette Wen Fang Wu Wu Mauricio Redondo-Solano Lidieth Uribe Rodolfo WingChing-Jones Jessie Usaga Natalia Barboza
author_facet	Jannette Wen Fang Wu Wu Mauricio Redondo-Solano Lidieth Uribe Rodolfo WingChing-Jones Jessie Usaga Natalia Barboza
author_sort	Jannette Wen Fang Wu Wu
title	First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
title_short	First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
title_full	First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
title_fullStr	First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
title_full_unstemmed	First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages
title_sort	first characterization of the probiotic potential of lactic acid bacteria isolated from costa rican pineapple silages
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/e5926cf25f684ecbae0cafe4d6be6435
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First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages

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