Increased activity of procoagulant factors in patients with small cell lung cancer.

Small cell lung cancer (SCLC) patients have augmented risk of developing venous thromboembolism, but the mechanisms triggering this burden on the coagulation system remain to be understood. Recently, cell-derived microparticles carrying procoagulant phospholipids (PPL) and tissue factor (TF) in thei...

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Autores principales:	Shona Pedersen, Anne Flou Kristensen, Ursula Falkmer, Gunna Christiansen, Søren Risom Kristensen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5944bdfb83b4c8b9bb3e2e9e0d318e8
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spelling	oai:doaj.org-article:e5944bdfb83b4c8b9bb3e2e9e0d318e82021-12-02T20:06:45ZIncreased activity of procoagulant factors in patients with small cell lung cancer.1932-620310.1371/journal.pone.0253613https://doaj.org/article/e5944bdfb83b4c8b9bb3e2e9e0d318e82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253613https://doaj.org/toc/1932-6203Small cell lung cancer (SCLC) patients have augmented risk of developing venous thromboembolism, but the mechanisms triggering this burden on the coagulation system remain to be understood. Recently, cell-derived microparticles carrying procoagulant phospholipids (PPL) and tissue factor (TF) in their membrane have attracted attention as possible contributors to the thrombogenic processes in cancers. The aims of this study were to assess the coagulation activity of platelet-poor plasma from 38 SCLC patients and to provide a detailed procoagulant profiling of small and large extracellular vesicles (EVs) isolated from these patients at the time of diagnosis, during and after treatment compared to 20 healthy controls. Hypercoagulability testing was performed by thrombin generation (TG), procoagulant phospholipid (PPL), TF activity, Protein C, FVIII activity and cell-free deoxyribonucleic acid (cfDNA), a surrogate measure for neutrophil extracellular traps (NETs). Our results revealed a coagulation activity that is significantly increased in the plasma of SCLC patients when compared to age-related healthy controls, but no substantial changes in coagulation activity during treatment and at follow-up. Although EVs in the patients revealed an increased PPL and TF activity compared with the controls, the TG profiles of EVs added to a standard plasma were similar for patients and controls. Finally, we found no differences in the coagulation profile of patients who developed VTE to those who did not, i.e. the tests could not predict VTE. In conclusion, we found that SCLC patients display an overall increased coagulation activity at time of diagnosis and during the disease, which may contribute to their higher risk of VTE.Shona PedersenAnne Flou KristensenUrsula FalkmerGunna ChristiansenSøren Risom KristensenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253613 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shona Pedersen Anne Flou Kristensen Ursula Falkmer Gunna Christiansen Søren Risom Kristensen Increased activity of procoagulant factors in patients with small cell lung cancer.
description	Small cell lung cancer (SCLC) patients have augmented risk of developing venous thromboembolism, but the mechanisms triggering this burden on the coagulation system remain to be understood. Recently, cell-derived microparticles carrying procoagulant phospholipids (PPL) and tissue factor (TF) in their membrane have attracted attention as possible contributors to the thrombogenic processes in cancers. The aims of this study were to assess the coagulation activity of platelet-poor plasma from 38 SCLC patients and to provide a detailed procoagulant profiling of small and large extracellular vesicles (EVs) isolated from these patients at the time of diagnosis, during and after treatment compared to 20 healthy controls. Hypercoagulability testing was performed by thrombin generation (TG), procoagulant phospholipid (PPL), TF activity, Protein C, FVIII activity and cell-free deoxyribonucleic acid (cfDNA), a surrogate measure for neutrophil extracellular traps (NETs). Our results revealed a coagulation activity that is significantly increased in the plasma of SCLC patients when compared to age-related healthy controls, but no substantial changes in coagulation activity during treatment and at follow-up. Although EVs in the patients revealed an increased PPL and TF activity compared with the controls, the TG profiles of EVs added to a standard plasma were similar for patients and controls. Finally, we found no differences in the coagulation profile of patients who developed VTE to those who did not, i.e. the tests could not predict VTE. In conclusion, we found that SCLC patients display an overall increased coagulation activity at time of diagnosis and during the disease, which may contribute to their higher risk of VTE.
format	article
author	Shona Pedersen Anne Flou Kristensen Ursula Falkmer Gunna Christiansen Søren Risom Kristensen
author_facet	Shona Pedersen Anne Flou Kristensen Ursula Falkmer Gunna Christiansen Søren Risom Kristensen
author_sort	Shona Pedersen
title	Increased activity of procoagulant factors in patients with small cell lung cancer.
title_short	Increased activity of procoagulant factors in patients with small cell lung cancer.
title_full	Increased activity of procoagulant factors in patients with small cell lung cancer.
title_fullStr	Increased activity of procoagulant factors in patients with small cell lung cancer.
title_full_unstemmed	Increased activity of procoagulant factors in patients with small cell lung cancer.
title_sort	increased activity of procoagulant factors in patients with small cell lung cancer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e5944bdfb83b4c8b9bb3e2e9e0d318e8
work_keys_str_mv	AT shonapedersen increasedactivityofprocoagulantfactorsinpatientswithsmallcelllungcancer AT annefloukristensen increasedactivityofprocoagulantfactorsinpatientswithsmallcelllungcancer AT ursulafalkmer increasedactivityofprocoagulantfactorsinpatientswithsmallcelllungcancer AT gunnachristiansen increasedactivityofprocoagulantfactorsinpatientswithsmallcelllungcancer AT sørenrisomkristensen increasedactivityofprocoagulantfactorsinpatientswithsmallcelllungcancer
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Increased activity of procoagulant factors in patients with small cell lung cancer.

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