Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum

Abstract Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish disease caused by Flavobacterium psychrophilum. In spite of its importance, an effective vaccine is not still available. Manipulation of the microbiome of skin, which is a prim...

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Autores principales:	Mio Takeuchi, Erina Fujiwara-Nagata, Taiki Katayama, Hiroaki Suetake
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e597e616e6e242599d9ea18a7001082c
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spelling	oai:doaj.org-article:e597e616e6e242599d9ea18a7001082c2021-12-02T14:17:27ZSkin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum10.1038/s41598-021-87167-12045-2322https://doaj.org/article/e597e616e6e242599d9ea18a7001082c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87167-1https://doaj.org/toc/2045-2322Abstract Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish disease caused by Flavobacterium psychrophilum. In spite of its importance, an effective vaccine is not still available. Manipulation of the microbiome of skin, which is a primary infection gate for pathogens, could be a novel countermeasure. For example, increasing the abundance of specific antagonistic bacteria against pathogens in fish skin might be effective to prevent fish disease. Here, we combined cultivation with 16S rRNA gene amplicon sequencing to obtain insight into the skin microbiome of the rainbow trout (Oncorhynchus mykiss) and searched for skin bacteria antagonistic to F. psychrophilum. By using multiple culture media, we obtained 174 isolates spanning 18 genera. Among them, Bosea sp. OX14 and Flavobacterium sp. GL7 respectively inhibited the growth of F. psychrophilum KU190628-78 and NCIMB 1947T, and produced antagonistic compounds of < 3 kDa in size. Sequences related to our isolates comprised 4.95% of skin microbial communities, and those related to strains OX14 and GL7 respectively comprised 1.60% and 0.17% of the skin microbiome. Comparisons with previously published microbiome data detected sequences related to strains OX14 and GL7 in skin of other rainbow trout and Atlantic salmon.Mio TakeuchiErina Fujiwara-NagataTaiki KatayamaHiroaki SuetakeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mio Takeuchi Erina Fujiwara-Nagata Taiki Katayama Hiroaki Suetake Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
description	Abstract Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish disease caused by Flavobacterium psychrophilum. In spite of its importance, an effective vaccine is not still available. Manipulation of the microbiome of skin, which is a primary infection gate for pathogens, could be a novel countermeasure. For example, increasing the abundance of specific antagonistic bacteria against pathogens in fish skin might be effective to prevent fish disease. Here, we combined cultivation with 16S rRNA gene amplicon sequencing to obtain insight into the skin microbiome of the rainbow trout (Oncorhynchus mykiss) and searched for skin bacteria antagonistic to F. psychrophilum. By using multiple culture media, we obtained 174 isolates spanning 18 genera. Among them, Bosea sp. OX14 and Flavobacterium sp. GL7 respectively inhibited the growth of F. psychrophilum KU190628-78 and NCIMB 1947T, and produced antagonistic compounds of < 3 kDa in size. Sequences related to our isolates comprised 4.95% of skin microbial communities, and those related to strains OX14 and GL7 respectively comprised 1.60% and 0.17% of the skin microbiome. Comparisons with previously published microbiome data detected sequences related to strains OX14 and GL7 in skin of other rainbow trout and Atlantic salmon.
format	article
author	Mio Takeuchi Erina Fujiwara-Nagata Taiki Katayama Hiroaki Suetake
author_facet	Mio Takeuchi Erina Fujiwara-Nagata Taiki Katayama Hiroaki Suetake
author_sort	Mio Takeuchi
title	Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
title_short	Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
title_full	Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
title_fullStr	Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
title_full_unstemmed	Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum
title_sort	skin bacteria of rainbow trout antagonistic to the fish pathogen flavobacterium psychrophilum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e597e616e6e242599d9ea18a7001082c
work_keys_str_mv	AT miotakeuchi skinbacteriaofrainbowtroutantagonistictothefishpathogenflavobacteriumpsychrophilum AT erinafujiwaranagata skinbacteriaofrainbowtroutantagonistictothefishpathogenflavobacteriumpsychrophilum AT taikikatayama skinbacteriaofrainbowtroutantagonistictothefishpathogenflavobacteriumpsychrophilum AT hiroakisuetake skinbacteriaofrainbowtroutantagonistictothefishpathogenflavobacteriumpsychrophilum
_version_	1718391639655841792

Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum

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