A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.

Bacterial genomic islands are often flanked by tRNA genes, which act as sites for the integration of foreign DNA into the host chromosome. For example, Bacillus cereus ATCC14579 contains a pathogenicity island flanked by a predicted pseudo-tRNA, tRNA(Other), which does not function in translation. D...

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Autores principales:	Theresa E Rogers, Sandro F Ataide, Kiley Dare, Assaf Katz, Stephanie Seveau, Hervé Roy, Michael Ibba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e59bc75d02f942a286378b2d20d7f204
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spelling	oai:doaj.org-article:e59bc75d02f942a286378b2d20d7f2042021-11-18T07:11:57ZA pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.1932-620310.1371/journal.pone.0041248https://doaj.org/article/e59bc75d02f942a286378b2d20d7f2042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22815980/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial genomic islands are often flanked by tRNA genes, which act as sites for the integration of foreign DNA into the host chromosome. For example, Bacillus cereus ATCC14579 contains a pathogenicity island flanked by a predicted pseudo-tRNA, tRNA(Other), which does not function in translation. Deletion of tRNA(Other) led to significant changes in cell wall morphology and antibiotic resistance and was accompanied by changes in the expression of numerous genes involved in oxidative stress responses, several of which contain significant complementarities to sequences surrounding tRNA(Other). This suggested that tRNA(Other) might be expressed as part of a larger RNA, and RACE analysis subsequently confirmed the existence of several RNA species that significantly extend both the 3' and 5'-ends of tRNA(Other). tRNA(Other) expression levels were found to be responsive to changes in extracellular iron concentration, consistent with the presence of three putative ferric uptake regulator (Fur) binding sites in the 5' leader region of one of these larger RNAs. Taken together with previous data, this study now suggests that tRNA(Other) may function by providing a tRNA-like structural element within a larger regulatory RNA. These findings illustrate that while integration of genomic islands often leaves tRNA genes intact and functional, in other instances inactivation may generate tRNA-like elements that are then recruited to other functions in the cell.Theresa E RogersSandro F AtaideKiley DareAssaf KatzStephanie SeveauHervé RoyMichael IbbaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41248 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Theresa E Rogers Sandro F Ataide Kiley Dare Assaf Katz Stephanie Seveau Hervé Roy Michael Ibba A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
description	Bacterial genomic islands are often flanked by tRNA genes, which act as sites for the integration of foreign DNA into the host chromosome. For example, Bacillus cereus ATCC14579 contains a pathogenicity island flanked by a predicted pseudo-tRNA, tRNA(Other), which does not function in translation. Deletion of tRNA(Other) led to significant changes in cell wall morphology and antibiotic resistance and was accompanied by changes in the expression of numerous genes involved in oxidative stress responses, several of which contain significant complementarities to sequences surrounding tRNA(Other). This suggested that tRNA(Other) might be expressed as part of a larger RNA, and RACE analysis subsequently confirmed the existence of several RNA species that significantly extend both the 3' and 5'-ends of tRNA(Other). tRNA(Other) expression levels were found to be responsive to changes in extracellular iron concentration, consistent with the presence of three putative ferric uptake regulator (Fur) binding sites in the 5' leader region of one of these larger RNAs. Taken together with previous data, this study now suggests that tRNA(Other) may function by providing a tRNA-like structural element within a larger regulatory RNA. These findings illustrate that while integration of genomic islands often leaves tRNA genes intact and functional, in other instances inactivation may generate tRNA-like elements that are then recruited to other functions in the cell.
format	article
author	Theresa E Rogers Sandro F Ataide Kiley Dare Assaf Katz Stephanie Seveau Hervé Roy Michael Ibba
author_facet	Theresa E Rogers Sandro F Ataide Kiley Dare Assaf Katz Stephanie Seveau Hervé Roy Michael Ibba
author_sort	Theresa E Rogers
title	A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
title_short	A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
title_full	A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
title_fullStr	A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
title_full_unstemmed	A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.
title_sort	pseudo-trna modulates antibiotic resistance in bacillus cereus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e59bc75d02f942a286378b2d20d7f204
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A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus.

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