Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.

<h4>Background</h4>Under the threat of global climatic change and food shortages, it is essential to take the initiative to obtain a comprehensive understanding of common and specific defence mechanisms existing in plant systems for protection against different types of biotic invaders....

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Autores principales:	Pankaj Barah, Per Winge, Anna Kusnierczyk, Diem Hong Tran, Atle M Bones
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e59ca05073984e8e9722d489a30a7e3d
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spelling	oai:doaj.org-article:e59ca05073984e8e9722d489a30a7e3d2021-11-18T07:52:10ZMolecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.1932-620310.1371/journal.pone.0058987https://doaj.org/article/e59ca05073984e8e9722d489a30a7e3d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23536844/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Under the threat of global climatic change and food shortages, it is essential to take the initiative to obtain a comprehensive understanding of common and specific defence mechanisms existing in plant systems for protection against different types of biotic invaders. We have implemented an integrated approach to analyse the overall transcriptomic reprogramming and systems-level defence responses in the model plant species Arabidopsis thaliana (A. thaliana henceforth) during insect Brevicoryne brassicae (B. brassicae henceforth) and bacterial Pseudomonas syringae pv. tomato strain DC3000 (P. syringae henceforth) attacks. The main aim of this study was to identify the attacker-specific and general defence response signatures in A. thaliana when attacked by phloem-feeding aphids or pathogenic bacteria.<h4>Results</h4>The obtained annotated networks of differentially expressed transcripts indicated that members of transcription factor families, such as WRKY, MYB, ERF, BHLH and bZIP, could be crucial for stress-specific defence regulation in Arabidopsis during aphid and P. syringae attack. The defence response pathways, signalling pathways and metabolic processes associated with aphid attack and P. syringae infection partially overlapped. Components of several important biosynthesis and signalling pathways, such as salicylic acid (SA), jasmonic acid (JA), ethylene (ET) and glucosinolates, were differentially affected during the two the treatments. Several stress-regulated transcription factors were known to be associated with stress-inducible microRNAs. The differentially regulated gene sets included many signature transcription factors, and our co-expression analysis showed that they were also strongly co-expressed during 69 other biotic stress experiments.<h4>Conclusions</h4>Defence responses and functional networks that were unique and specific to aphid or P. syringae stresses were identified. Furthermore, our analysis revealed a probable link between biotic stress and microRNAs in Arabidopsis and, thus gives indicates a new direction for conducting large-scale targeted experiments to explore the detailed regulatory links between them. The presented results provide a comparative understanding of Arabidopsis - B. brassicae and Arabidopsis - P. syringae interactions at the transcriptomic level.Pankaj BarahPer WingeAnna KusnierczykDiem Hong TranAtle M BonesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58987 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pankaj Barah Per Winge Anna Kusnierczyk Diem Hong Tran Atle M Bones Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
description	<h4>Background</h4>Under the threat of global climatic change and food shortages, it is essential to take the initiative to obtain a comprehensive understanding of common and specific defence mechanisms existing in plant systems for protection against different types of biotic invaders. We have implemented an integrated approach to analyse the overall transcriptomic reprogramming and systems-level defence responses in the model plant species Arabidopsis thaliana (A. thaliana henceforth) during insect Brevicoryne brassicae (B. brassicae henceforth) and bacterial Pseudomonas syringae pv. tomato strain DC3000 (P. syringae henceforth) attacks. The main aim of this study was to identify the attacker-specific and general defence response signatures in A. thaliana when attacked by phloem-feeding aphids or pathogenic bacteria.<h4>Results</h4>The obtained annotated networks of differentially expressed transcripts indicated that members of transcription factor families, such as WRKY, MYB, ERF, BHLH and bZIP, could be crucial for stress-specific defence regulation in Arabidopsis during aphid and P. syringae attack. The defence response pathways, signalling pathways and metabolic processes associated with aphid attack and P. syringae infection partially overlapped. Components of several important biosynthesis and signalling pathways, such as salicylic acid (SA), jasmonic acid (JA), ethylene (ET) and glucosinolates, were differentially affected during the two the treatments. Several stress-regulated transcription factors were known to be associated with stress-inducible microRNAs. The differentially regulated gene sets included many signature transcription factors, and our co-expression analysis showed that they were also strongly co-expressed during 69 other biotic stress experiments.<h4>Conclusions</h4>Defence responses and functional networks that were unique and specific to aphid or P. syringae stresses were identified. Furthermore, our analysis revealed a probable link between biotic stress and microRNAs in Arabidopsis and, thus gives indicates a new direction for conducting large-scale targeted experiments to explore the detailed regulatory links between them. The presented results provide a comparative understanding of Arabidopsis - B. brassicae and Arabidopsis - P. syringae interactions at the transcriptomic level.
format	article
author	Pankaj Barah Per Winge Anna Kusnierczyk Diem Hong Tran Atle M Bones
author_facet	Pankaj Barah Per Winge Anna Kusnierczyk Diem Hong Tran Atle M Bones
author_sort	Pankaj Barah
title	Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
title_short	Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
title_full	Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
title_fullStr	Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
title_full_unstemmed	Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.
title_sort	molecular signatures in arabidopsis thaliana in response to insect attack and bacterial infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e59ca05073984e8e9722d489a30a7e3d
work_keys_str_mv	AT pankajbarah molecularsignaturesinarabidopsisthalianainresponsetoinsectattackandbacterialinfection AT perwinge molecularsignaturesinarabidopsisthalianainresponsetoinsectattackandbacterialinfection AT annakusnierczyk molecularsignaturesinarabidopsisthalianainresponsetoinsectattackandbacterialinfection AT diemhongtran molecularsignaturesinarabidopsisthalianainresponsetoinsectattackandbacterialinfection AT atlembones molecularsignaturesinarabidopsisthalianainresponsetoinsectattackandbacterialinfection
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Molecular signatures in Arabidopsis thaliana in response to insect attack and bacterial infection.

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