High shear resistance of insect cells: the basis for substantial improvements in cell culture process design

Abstract Multicellular organisms cultivated in continuous stirred tank reactors (CSTRs) are more sensitive to environmental conditions in the suspension culture than microbial cells. The hypothesis, that stirring induced shear stress is the main problem, persists, although it has been shown that the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Florian Strobl, Mark Duerkop, Dieter Palmberger, Gerald Striedner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e59eb6a90fce4ebab6df21893a73bc19
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e59eb6a90fce4ebab6df21893a73bc19
record_format	dspace
spelling	oai:doaj.org-article:e59eb6a90fce4ebab6df21893a73bc192021-12-02T14:41:52ZHigh shear resistance of insect cells: the basis for substantial improvements in cell culture process design10.1038/s41598-021-88813-42045-2322https://doaj.org/article/e59eb6a90fce4ebab6df21893a73bc192021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88813-4https://doaj.org/toc/2045-2322Abstract Multicellular organisms cultivated in continuous stirred tank reactors (CSTRs) are more sensitive to environmental conditions in the suspension culture than microbial cells. The hypothesis, that stirring induced shear stress is the main problem, persists, although it has been shown that these cells are not so sensitive to shear. As these results are largely based on Chinese Hamster Ovary (CHO) cell experiments the question remains if similar behavior is valid for insect cells with a higher specific oxygen demand. The requirement of higher oxygen transfer rates is associated with higher shear forces in the process. Consequently, we focused on the shear resistance of insect cells, using CHO cells as reference system. We applied a microfluidic device that allowed defined variations in shear rates. Both cell lines displayed high resistance to shear rates up to 8.73 × 105 s−1. Based on these results we used microbial CSTRs, operated at high revolution speeds and low aeration rates and found no negative impact on cell viability. Further, this cultivation approach led to substantially reduced gas flow rates, gas bubble and foam formation, while addition of pure oxygen was no longer necessary. Therefore, this study contributes to the development of more robust insect cell culture processes.Florian StroblMark DuerkopDieter PalmbergerGerald StriednerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florian Strobl Mark Duerkop Dieter Palmberger Gerald Striedner High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
description	Abstract Multicellular organisms cultivated in continuous stirred tank reactors (CSTRs) are more sensitive to environmental conditions in the suspension culture than microbial cells. The hypothesis, that stirring induced shear stress is the main problem, persists, although it has been shown that these cells are not so sensitive to shear. As these results are largely based on Chinese Hamster Ovary (CHO) cell experiments the question remains if similar behavior is valid for insect cells with a higher specific oxygen demand. The requirement of higher oxygen transfer rates is associated with higher shear forces in the process. Consequently, we focused on the shear resistance of insect cells, using CHO cells as reference system. We applied a microfluidic device that allowed defined variations in shear rates. Both cell lines displayed high resistance to shear rates up to 8.73 × 105 s−1. Based on these results we used microbial CSTRs, operated at high revolution speeds and low aeration rates and found no negative impact on cell viability. Further, this cultivation approach led to substantially reduced gas flow rates, gas bubble and foam formation, while addition of pure oxygen was no longer necessary. Therefore, this study contributes to the development of more robust insect cell culture processes.
format	article
author	Florian Strobl Mark Duerkop Dieter Palmberger Gerald Striedner
author_facet	Florian Strobl Mark Duerkop Dieter Palmberger Gerald Striedner
author_sort	Florian Strobl
title	High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
title_short	High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
title_full	High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
title_fullStr	High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
title_full_unstemmed	High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
title_sort	high shear resistance of insect cells: the basis for substantial improvements in cell culture process design
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e59eb6a90fce4ebab6df21893a73bc19
work_keys_str_mv	AT florianstrobl highshearresistanceofinsectcellsthebasisforsubstantialimprovementsincellcultureprocessdesign AT markduerkop highshearresistanceofinsectcellsthebasisforsubstantialimprovementsincellcultureprocessdesign AT dieterpalmberger highshearresistanceofinsectcellsthebasisforsubstantialimprovementsincellcultureprocessdesign AT geraldstriedner highshearresistanceofinsectcellsthebasisforsubstantialimprovementsincellcultureprocessdesign
_version_	1718389904507928576

High shear resistance of insect cells: the basis for substantial improvements in cell culture process design

Ejemplares similares