The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides

Abstract The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and...

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Autores principales:	Filip Bartnicki, Piotr Bonarek, Ewa Kowalska, Wojciech Strzalka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5a08da97ae845e686c793c2e5807789
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spelling	oai:doaj.org-article:e5a08da97ae845e686c793c2e58077892021-12-02T16:05:59ZThe Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides10.1038/s41598-017-02432-62045-2322https://doaj.org/article/e5a08da97ae845e686c793c2e58077892017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02432-6https://doaj.org/toc/2045-2322Abstract The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.Filip BartnickiPiotr BonarekEwa KowalskaWojciech StrzalkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Filip Bartnicki Piotr Bonarek Ewa Kowalska Wojciech Strzalka The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
description	Abstract The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.
format	article
author	Filip Bartnicki Piotr Bonarek Ewa Kowalska Wojciech Strzalka
author_facet	Filip Bartnicki Piotr Bonarek Ewa Kowalska Wojciech Strzalka
author_sort	Filip Bartnicki
title	The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_short	The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_full	The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_fullStr	The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_full_unstemmed	The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_sort	argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e5a08da97ae845e686c793c2e5807789
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The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides

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