Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells

Actomyosin contractility regulates various cellular processes including proliferation and differentiation while dysregulation of actomyosin activity contributes to cancer development and progression. Previously, we have reported that actomyosin-generated tension at adherens junctions is required for...

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Autores principales:	Oleg Dobrokhotov, Masaki Sunagawa, Takeru Torii, Shinji Mii, Keiko Kawauchi, Atsushi Enomoto, Masahiro Sokabe, Hiroaki Hirata
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	actomyosin adherens junction contact inhibition mechanosensing mechanotransduction epidermoid carcinoma Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e5a1bf86e04c4c7e80dd0a75ec3d5234
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spelling	oai:doaj.org-article:e5a1bf86e04c4c7e80dd0a75ec3d52342021-11-11T08:04:10ZAnti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells2296-634X10.3389/fcell.2021.728383https://doaj.org/article/e5a1bf86e04c4c7e80dd0a75ec3d52342021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.728383/fullhttps://doaj.org/toc/2296-634XActomyosin contractility regulates various cellular processes including proliferation and differentiation while dysregulation of actomyosin activity contributes to cancer development and progression. Previously, we have reported that actomyosin-generated tension at adherens junctions is required for cell density-dependent inhibition of proliferation of normal skin keratinocytes. However, it remains unclear how actomyosin contractility affects the hyperproliferation ability of cutaneous squamous cell carcinoma (cSCC) cells. In this study, we find that actomyosin activity is impaired in cSCC cells both in vitro and in vivo. External application of tensile loads to adherens junctions by sustained mechanical stretch attenuates the proliferation of cSCC cells, which depends on intact adherens junctions. Forced activation of actomyosin of cSCC cells also inhibits their proliferation in a cell-cell contact-dependent manner. Furthermore, the cell cycle arrest induced by tensile loading to adherens junctions is accompanied by epidermal differentiation in cSCC cells. Our results show that the degree of malignant properties of cSCC cells can be reduced by applying tensile loads to adherens junctions, which implies that the mechanical status of adherens junctions may serve as a novel therapeutic target for cSCC.Oleg DobrokhotovMasaki SunagawaMasaki SunagawaTakeru ToriiShinji MiiKeiko KawauchiAtsushi EnomotoMasahiro SokabeHiroaki HirataFrontiers Media S.A.articleactomyosinadherens junctioncontact inhibitionmechanosensingmechanotransductionepidermoid carcinomaBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	actomyosin adherens junction contact inhibition mechanosensing mechanotransduction epidermoid carcinoma Biology (General) QH301-705.5
spellingShingle	actomyosin adherens junction contact inhibition mechanosensing mechanotransduction epidermoid carcinoma Biology (General) QH301-705.5 Oleg Dobrokhotov Masaki Sunagawa Masaki Sunagawa Takeru Torii Shinji Mii Keiko Kawauchi Atsushi Enomoto Masahiro Sokabe Hiroaki Hirata Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
description	Actomyosin contractility regulates various cellular processes including proliferation and differentiation while dysregulation of actomyosin activity contributes to cancer development and progression. Previously, we have reported that actomyosin-generated tension at adherens junctions is required for cell density-dependent inhibition of proliferation of normal skin keratinocytes. However, it remains unclear how actomyosin contractility affects the hyperproliferation ability of cutaneous squamous cell carcinoma (cSCC) cells. In this study, we find that actomyosin activity is impaired in cSCC cells both in vitro and in vivo. External application of tensile loads to adherens junctions by sustained mechanical stretch attenuates the proliferation of cSCC cells, which depends on intact adherens junctions. Forced activation of actomyosin of cSCC cells also inhibits their proliferation in a cell-cell contact-dependent manner. Furthermore, the cell cycle arrest induced by tensile loading to adherens junctions is accompanied by epidermal differentiation in cSCC cells. Our results show that the degree of malignant properties of cSCC cells can be reduced by applying tensile loads to adherens junctions, which implies that the mechanical status of adherens junctions may serve as a novel therapeutic target for cSCC.
format	article
author	Oleg Dobrokhotov Masaki Sunagawa Masaki Sunagawa Takeru Torii Shinji Mii Keiko Kawauchi Atsushi Enomoto Masahiro Sokabe Hiroaki Hirata
author_facet	Oleg Dobrokhotov Masaki Sunagawa Masaki Sunagawa Takeru Torii Shinji Mii Keiko Kawauchi Atsushi Enomoto Masahiro Sokabe Hiroaki Hirata
author_sort	Oleg Dobrokhotov
title	Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
title_short	Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
title_full	Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
title_fullStr	Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
title_full_unstemmed	Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells
title_sort	anti-malignant effect of tensile loading to adherens junctions in cutaneous squamous cell carcinoma cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e5a1bf86e04c4c7e80dd0a75ec3d5234
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Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells

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