Zeptomole electrochemical detection of metallothioneins.

<h4>Background</h4>Thiol-rich peptides and proteins possess a large number of biological activities and may serve as markers for numerous health problems including cancer. Metallothionein (MT), a small molecular mass protein rich in cysteine, may be considered as one of the promising tum...

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Autores principales:	Vojtech Adam, Jitka Petrlova, Joseph Wang, Tomas Eckschlager, Libuse Trnkova, Rene Kizek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5ab660a411243648ae28be1c96a6763
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spelling	oai:doaj.org-article:e5ab660a411243648ae28be1c96a67632021-12-02T20:20:18ZZeptomole electrochemical detection of metallothioneins.1932-620310.1371/journal.pone.0011441https://doaj.org/article/e5ab660a411243648ae28be1c96a67632010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20625429/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Thiol-rich peptides and proteins possess a large number of biological activities and may serve as markers for numerous health problems including cancer. Metallothionein (MT), a small molecular mass protein rich in cysteine, may be considered as one of the promising tumour markers. The aim of this paper was to employ chronopotentiometric stripping analysis (CPSA) for highly sensitive detection of MT.<h4>Methodology/principal findings</h4>In this study, we used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein. Under the optimal conditions, we were able to estimate detection limits down to tens of fg per ml. Further, this method was applied to detect metallothioneins in blood serum obtained from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin in order to show the possible role of metallothioneins in carcinogenesis. It was found that MT level in blood serum was almost twice higher as compared to the level determined in healthy individuals.<h4>Conclusions/significance</h4>This paper brings unique results on the application of ultra-sensitive electroanalytical method for metallothionein detection. The detection limit and other analytical parameters are the best among the parameters of other techniques. In spite of the fact that the paper is mainly focused on metallothionein, it is worth mentioning that successful detection of other biologically important molecules is possible by this method. Coupling of this method with simple isolation methods such as antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument.Vojtech AdamJitka PetrlovaJoseph WangTomas EckschlagerLibuse TrnkovaRene KizekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11441 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vojtech Adam Jitka Petrlova Joseph Wang Tomas Eckschlager Libuse Trnkova Rene Kizek Zeptomole electrochemical detection of metallothioneins.
description	<h4>Background</h4>Thiol-rich peptides and proteins possess a large number of biological activities and may serve as markers for numerous health problems including cancer. Metallothionein (MT), a small molecular mass protein rich in cysteine, may be considered as one of the promising tumour markers. The aim of this paper was to employ chronopotentiometric stripping analysis (CPSA) for highly sensitive detection of MT.<h4>Methodology/principal findings</h4>In this study, we used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein. Under the optimal conditions, we were able to estimate detection limits down to tens of fg per ml. Further, this method was applied to detect metallothioneins in blood serum obtained from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin in order to show the possible role of metallothioneins in carcinogenesis. It was found that MT level in blood serum was almost twice higher as compared to the level determined in healthy individuals.<h4>Conclusions/significance</h4>This paper brings unique results on the application of ultra-sensitive electroanalytical method for metallothionein detection. The detection limit and other analytical parameters are the best among the parameters of other techniques. In spite of the fact that the paper is mainly focused on metallothionein, it is worth mentioning that successful detection of other biologically important molecules is possible by this method. Coupling of this method with simple isolation methods such as antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument.
format	article
author	Vojtech Adam Jitka Petrlova Joseph Wang Tomas Eckschlager Libuse Trnkova Rene Kizek
author_facet	Vojtech Adam Jitka Petrlova Joseph Wang Tomas Eckschlager Libuse Trnkova Rene Kizek
author_sort	Vojtech Adam
title	Zeptomole electrochemical detection of metallothioneins.
title_short	Zeptomole electrochemical detection of metallothioneins.
title_full	Zeptomole electrochemical detection of metallothioneins.
title_fullStr	Zeptomole electrochemical detection of metallothioneins.
title_full_unstemmed	Zeptomole electrochemical detection of metallothioneins.
title_sort	zeptomole electrochemical detection of metallothioneins.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e5ab660a411243648ae28be1c96a6763
work_keys_str_mv	AT vojtechadam zeptomoleelectrochemicaldetectionofmetallothioneins AT jitkapetrlova zeptomoleelectrochemicaldetectionofmetallothioneins AT josephwang zeptomoleelectrochemicaldetectionofmetallothioneins AT tomaseckschlager zeptomoleelectrochemicaldetectionofmetallothioneins AT libusetrnkova zeptomoleelectrochemicaldetectionofmetallothioneins AT renekizek zeptomoleelectrochemicaldetectionofmetallothioneins
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Zeptomole electrochemical detection of metallothioneins.

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