Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model

Abstract To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after tra...

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Autores principales:	Xifu Shang, Bing Shu, Yongjun Wang, Zhengliang Luo, Guangxi Wang, Shane Barton, Massimo Max Morandi, Christopher Kevil, Yufeng Dong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5be3fda54b74943b25b37f751aab3fa
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spelling	oai:doaj.org-article:e5be3fda54b74943b25b37f751aab3fa2021-12-02T15:06:06ZHuman Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model10.1038/s41598-017-08804-22045-2322https://doaj.org/article/e5be3fda54b74943b25b37f751aab3fa2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08804-2https://doaj.org/toc/2045-2322Abstract To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantation of MSC sheets, results showed more bony callus formed between allograft and host bone ends in both young P3 MSC and aged P10 MSC sheet-wrapped groups when compared to allograft alone. At 6 weeks, while both MSC sheet-wrapped allografts showed more bony callus formation when compared to allograft alone groups, the bony callus size in aged P10 MSC sheet groups was significantly less than young P3 MSC sheet groups. Biomechanical testing confirmed that P3 MSC sheet-grafted femurs had the highest biomechanical strength in the three groups. Histology sections showed that the area of the chondriod callus in the aged P10 MSC sheet groups was significantly larger than in P3 MSC sheet groups. Finally, a significant increase of chondro-osteoclast activity was observed in the P3 MSC sheet-grafted femur. Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteogenic ability and subsequent bony callus formation, and could be used to induce cartilaginous callus formation.Xifu ShangBing ShuYongjun WangZhengliang LuoGuangxi WangShane BartonMassimo Max MorandiChristopher KevilYufeng DongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xifu Shang Bing Shu Yongjun Wang Zhengliang Luo Guangxi Wang Shane Barton Massimo Max Morandi Christopher Kevil Yufeng Dong Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
description	Abstract To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantation of MSC sheets, results showed more bony callus formed between allograft and host bone ends in both young P3 MSC and aged P10 MSC sheet-wrapped groups when compared to allograft alone. At 6 weeks, while both MSC sheet-wrapped allografts showed more bony callus formation when compared to allograft alone groups, the bony callus size in aged P10 MSC sheet groups was significantly less than young P3 MSC sheet groups. Biomechanical testing confirmed that P3 MSC sheet-grafted femurs had the highest biomechanical strength in the three groups. Histology sections showed that the area of the chondriod callus in the aged P10 MSC sheet groups was significantly larger than in P3 MSC sheet groups. Finally, a significant increase of chondro-osteoclast activity was observed in the P3 MSC sheet-grafted femur. Our data demonstrates that extensive long-term culture-induced MSC aging impaired their osteogenic ability and subsequent bony callus formation, and could be used to induce cartilaginous callus formation.
format	article
author	Xifu Shang Bing Shu Yongjun Wang Zhengliang Luo Guangxi Wang Shane Barton Massimo Max Morandi Christopher Kevil Yufeng Dong
author_facet	Xifu Shang Bing Shu Yongjun Wang Zhengliang Luo Guangxi Wang Shane Barton Massimo Max Morandi Christopher Kevil Yufeng Dong
author_sort	Xifu Shang
title	Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
title_short	Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
title_full	Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
title_fullStr	Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
title_full_unstemmed	Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model
title_sort	human mesenchymal stromal cell sheet enhances allograft repair in a mouse model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e5be3fda54b74943b25b37f751aab3fa
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Human Mesenchymal Stromal Cell Sheet Enhances Allograft Repair in a Mouse Model

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